Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gutM and srlR are separated by 66 nucleotides srlR and gutQ overlap by 8 nucleotides gutQ and ygaA overlap by 4 nucleotides
b2706: gutM - DNA-binding transcriptional activator of glucitol operon (NCBI), at 2,826,643 to 2,827,002
gutM
b2707: srlR - DNA-bindng transcriptional repressor (NCBI), at 2,827,069 to 2,827,842
srlR
b2708: gutQ - orf, hypothetical protein (VIMSS), at 2,827,835 to 2,828,800
gutQ
b2709: ygaA - putative 2-component transcriptional regulator (VIMSS), at 2,828,797 to 2,830,311
ygaA
Position (kb)
2827
2828
2829 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2826.876 kb on - strand, within gutM at 2826.932 kb on + strand, within gutM at 2826.963 kb on - strand, within gutM at 2827.166 kb on - strand, within srlR at 2827.166 kb on - strand, within srlR at 2827.191 kb on - strand, within srlR at 2827.191 kb on - strand, within srlR at 2827.193 kb on + strand, within srlR at 2827.193 kb on + strand, within srlR at 2827.201 kb on - strand, within srlR at 2827.298 kb on + strand, within srlR at 2827.368 kb on + strand, within srlR at 2827.425 kb on + strand, within srlR at 2827.431 kb on + strand, within srlR at 2827.481 kb on + strand, within srlR at 2827.483 kb on - strand, within srlR at 2827.549 kb on + strand, within srlR at 2827.619 kb on + strand, within srlR at 2827.619 kb on + strand, within srlR at 2827.893 kb on - strand at 2828.144 kb on + strand, within gutQ at 2828.242 kb on - strand, within gutQ at 2828.313 kb on - strand, within gutQ at 2828.314 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.394 kb on - strand, within gutQ at 2828.405 kb on - strand, within gutQ at 2829.156 kb on - strand, within ygaA at 2829.156 kb on - strand, within ygaA at 2829.382 kb on + strand, within ygaA at 2829.382 kb on + strand, within ygaA at 2829.400 kb on + strand, within ygaA at 2829.417 kb on + strand, within ygaA at 2829.527 kb on + strand, within ygaA at 2829.527 kb on + strand, within ygaA at 2829.544 kb on - strand, within ygaA at 2829.583 kb on + strand, within ygaA at 2829.583 kb on + strand, within ygaA at 2829.687 kb on + strand, within ygaA at 2829.687 kb on + strand, within ygaA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 2,826,876 - gutM b2706 0.65 -0.3 2,826,932 + gutM b2706 0.80 -0.2 2,826,963 - gutM b2706 0.89 -0.8 2,827,166 - srlR b2707 0.13 +1.4 2,827,166 - srlR b2707 0.13 +0.6 2,827,191 - srlR b2707 0.16 +0.1 2,827,191 - srlR b2707 0.16 +2.3 2,827,193 + srlR b2707 0.16 -1.9 2,827,193 + srlR b2707 0.16 -0.7 2,827,201 - srlR b2707 0.17 +0.9 2,827,298 + srlR b2707 0.30 +0.1 2,827,368 + srlR b2707 0.39 -1.2 2,827,425 + srlR b2707 0.46 -0.1 2,827,431 + srlR b2707 0.47 -0.9 2,827,481 + srlR b2707 0.53 +1.8 2,827,483 - srlR b2707 0.53 +0.7 2,827,549 + srlR b2707 0.62 -0.4 2,827,619 + srlR b2707 0.71 -0.2 2,827,619 + srlR b2707 0.71 -0.2 2,827,893 - +3.0 2,828,144 + gutQ b2708 0.32 +1.0 2,828,242 - gutQ b2708 0.42 +2.7 2,828,313 - gutQ b2708 0.49 +1.2 2,828,314 - gutQ b2708 0.50 +1.7 2,828,394 - gutQ b2708 0.58 +2.1 2,828,394 - gutQ b2708 0.58 +2.7 2,828,394 - gutQ b2708 0.58 +1.8 2,828,405 - gutQ b2708 0.59 +2.5 2,829,156 - ygaA b2709 0.24 +1.2 2,829,156 - ygaA b2709 0.24 -1.8 2,829,382 + ygaA b2709 0.39 +0.9 2,829,382 + ygaA b2709 0.39 +1.9 2,829,400 + ygaA b2709 0.40 -0.5 2,829,417 + ygaA b2709 0.41 -1.1 2,829,527 + ygaA b2709 0.48 -1.1 2,829,527 + ygaA b2709 0.48 -0.3 2,829,544 - ygaA b2709 0.49 +0.6 2,829,583 + ygaA b2709 0.52 +0.6 2,829,583 + ygaA b2709 0.52 +0.5 2,829,687 + ygaA b2709 0.59 +0.3 2,829,687 + ygaA b2709 0.59 +0.2
Or see this region's nucleotide sequence