Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt eutE and cchB are separated by 11 nucleotides cchB and cchA are separated by 106 nucleotides cchA and eutI are separated by 38 nucleotides eutI and eutT overlap by 4 nucleotides eutT and eutQ overlap by 4 nucleotides
b2455: eutE - predicted aldehyde dehydrogenase, ethanolamine utilization protein (NCBI), at 2,568,370 to 2,569,773
eutE
b2456: cchB - predicted carboxysome structural protein, ethanolamine utilization protein (NCBI), at 2,569,785 to 2,570,072
cchB
b2457: cchA - detox protein (VIMSS), at 2,570,179 to 2,570,472
cchA
b2458: eutI - predicted phosphotransacetylase subunit (NCBI), at 2,570,511 to 2,571,527
eutI
b2459: eutT - predicted cobalamin adenosyltransferase in ethanolamine utilization (NCBI), at 2,571,524 to 2,572,327
eutT
b2460: eutQ - hypothetical protein (NCBI), at 2,572,324 to 2,573,025
eutQ
Position (kb)
2570
2571
2572 Strain fitness (log2 ratio)
-2
-1
0
1 at 2569.734 kb on + strand at 2569.820 kb on + strand, within cchB at 2569.837 kb on + strand, within cchB at 2569.849 kb on - strand, within cchB at 2569.849 kb on - strand, within cchB at 2570.117 kb on - strand at 2570.168 kb on + strand at 2570.168 kb on + strand at 2570.261 kb on - strand, within cchA at 2570.318 kb on - strand, within cchA at 2570.350 kb on + strand, within cchA at 2570.353 kb on - strand, within cchA at 2570.353 kb on - strand, within cchA at 2570.368 kb on - strand, within cchA at 2570.435 kb on + strand, within cchA at 2570.435 kb on + strand, within cchA at 2570.490 kb on - strand at 2570.502 kb on - strand at 2570.654 kb on - strand, within eutI at 2570.989 kb on + strand, within eutI at 2570.989 kb on + strand, within eutI at 2571.039 kb on + strand, within eutI at 2571.065 kb on - strand, within eutI at 2571.148 kb on + strand, within eutI at 2571.148 kb on + strand, within eutI at 2571.330 kb on - strand, within eutI at 2571.337 kb on - strand, within eutI at 2571.337 kb on - strand, within eutI at 2571.441 kb on + strand at 2571.561 kb on + strand at 2571.561 kb on + strand at 2571.564 kb on - strand at 2571.599 kb on + strand at 2571.603 kb on - strand at 2571.603 kb on - strand at 2571.655 kb on + strand, within eutT at 2571.812 kb on + strand, within eutT at 2571.924 kb on + strand, within eutT at 2571.924 kb on + strand, within eutT at 2571.985 kb on - strand, within eutT at 2571.985 kb on - strand, within eutT at 2572.113 kb on + strand, within eutT at 2572.134 kb on + strand, within eutT at 2572.134 kb on + strand, within eutT at 2572.153 kb on - strand, within eutT at 2572.405 kb on + strand, within eutQ at 2572.471 kb on - strand, within eutQ at 2572.471 kb on - strand, within eutQ at 2572.477 kb on + strand, within eutQ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 2,569,734 + -1.8 2,569,820 + cchB b2456 0.12 -2.2 2,569,837 + cchB b2456 0.18 -1.0 2,569,849 - cchB b2456 0.22 -0.8 2,569,849 - cchB b2456 0.22 -0.8 2,570,117 - +0.2 2,570,168 + +0.9 2,570,168 + +0.3 2,570,261 - cchA b2457 0.28 -0.6 2,570,318 - cchA b2457 0.47 -0.4 2,570,350 + cchA b2457 0.58 -2.0 2,570,353 - cchA b2457 0.59 -0.7 2,570,353 - cchA b2457 0.59 +0.3 2,570,368 - cchA b2457 0.64 -0.2 2,570,435 + cchA b2457 0.87 +0.8 2,570,435 + cchA b2457 0.87 -0.8 2,570,490 - -0.4 2,570,502 - +1.2 2,570,654 - eutI b2458 0.14 +1.3 2,570,989 + eutI b2458 0.47 +1.4 2,570,989 + eutI b2458 0.47 +0.9 2,571,039 + eutI b2458 0.52 +1.4 2,571,065 - eutI b2458 0.54 -1.0 2,571,148 + eutI b2458 0.63 -0.6 2,571,148 + eutI b2458 0.63 +0.5 2,571,330 - eutI b2458 0.81 +0.8 2,571,337 - eutI b2458 0.81 -0.5 2,571,337 - eutI b2458 0.81 -0.1 2,571,441 + -1.4 2,571,561 + -0.6 2,571,561 + -1.0 2,571,564 - +0.3 2,571,599 + +0.8 2,571,603 - +0.4 2,571,603 - +0.6 2,571,655 + eutT b2459 0.16 -0.3 2,571,812 + eutT b2459 0.36 -0.3 2,571,924 + eutT b2459 0.50 -1.5 2,571,924 + eutT b2459 0.50 +0.1 2,571,985 - eutT b2459 0.57 +1.1 2,571,985 - eutT b2459 0.57 -1.2 2,572,113 + eutT b2459 0.73 +0.4 2,572,134 + eutT b2459 0.76 -0.1 2,572,134 + eutT b2459 0.76 -1.0 2,572,153 - eutT b2459 0.78 +0.6 2,572,405 + eutQ b2460 0.12 -0.3 2,572,471 - eutQ b2460 0.21 +0.7 2,572,471 - eutQ b2460 0.21 -1.0 2,572,477 + eutQ b2460 0.22 -0.4
Or see this region's nucleotide sequence