Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt vacJ and yfdC are separated by 293 nucleotides yfdC and argW are separated by 75 nucleotides argW and intS are separated by 161 nucleotides
b2346: vacJ - predicted lipoprotein (NCBI), at 2,462,274 to 2,463,029
vacJ
b2347: yfdC - predicted inner membrane protein (NCBI), at 2,463,323 to 2,464,255
yfdC
b2348: argW - tRNA-Arg (NCBI), at 2,464,331 to 2,464,405
argW
b2349: intS - CPS-53 (KpLE1) prophage; predicted prophage CPS-53 integrase (NCBI), at 2,464,567 to 2,465,724
intS
Position (kb)
2463
2464
2465 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2462.333 kb on - strand at 2462.336 kb on - strand at 2462.344 kb on - strand at 2462.344 kb on - strand at 2462.394 kb on - strand, within vacJ at 2462.399 kb on - strand, within vacJ at 2462.403 kb on - strand, within vacJ at 2462.405 kb on - strand, within vacJ at 2462.406 kb on - strand, within vacJ at 2462.421 kb on - strand, within vacJ at 2462.438 kb on - strand, within vacJ at 2462.447 kb on + strand, within vacJ at 2462.465 kb on + strand, within vacJ at 2462.471 kb on - strand, within vacJ at 2462.491 kb on - strand, within vacJ at 2462.507 kb on - strand, within vacJ at 2462.619 kb on + strand, within vacJ at 2462.619 kb on + strand, within vacJ at 2462.623 kb on + strand, within vacJ at 2462.708 kb on - strand, within vacJ at 2462.799 kb on - strand, within vacJ at 2462.878 kb on + strand, within vacJ at 2462.909 kb on + strand, within vacJ at 2462.913 kb on - strand, within vacJ at 2463.052 kb on + strand at 2463.052 kb on + strand at 2463.126 kb on + strand at 2463.170 kb on + strand at 2463.186 kb on + strand at 2463.194 kb on - strand at 2463.365 kb on - strand at 2463.415 kb on - strand at 2463.431 kb on - strand, within yfdC at 2463.473 kb on + strand, within yfdC at 2463.568 kb on - strand, within yfdC at 2463.568 kb on - strand, within yfdC at 2463.648 kb on - strand, within yfdC at 2463.683 kb on + strand, within yfdC at 2463.728 kb on + strand, within yfdC at 2463.736 kb on + strand, within yfdC at 2463.736 kb on + strand, within yfdC at 2463.777 kb on + strand, within yfdC at 2463.807 kb on - strand, within yfdC at 2463.807 kb on - strand, within yfdC at 2463.811 kb on + strand, within yfdC at 2463.855 kb on - strand, within yfdC at 2463.858 kb on + strand, within yfdC at 2463.858 kb on + strand, within yfdC at 2463.861 kb on - strand, within yfdC at 2463.861 kb on - strand, within yfdC at 2463.868 kb on - strand, within yfdC at 2463.926 kb on - strand, within yfdC at 2463.980 kb on + strand, within yfdC at 2463.984 kb on + strand, within yfdC at 2463.997 kb on + strand, within yfdC at 2464.005 kb on - strand, within yfdC at 2464.005 kb on - strand, within yfdC at 2464.007 kb on + strand, within yfdC at 2464.059 kb on + strand, within yfdC at 2464.059 kb on - strand, within yfdC at 2464.094 kb on - strand, within yfdC at 2464.127 kb on + strand, within yfdC at 2464.149 kb on + strand, within yfdC at 2464.177 kb on - strand at 2464.312 kb on + strand at 2464.312 kb on + strand at 2464.320 kb on - strand at 2464.427 kb on + strand at 2464.515 kb on + strand at 2464.592 kb on - strand at 2464.644 kb on - strand at 2464.644 kb on - strand at 2464.646 kb on + strand at 2464.764 kb on + strand, within intS at 2464.772 kb on - strand, within intS at 2464.813 kb on - strand, within intS at 2464.875 kb on + strand, within intS at 2464.875 kb on + strand, within intS at 2465.006 kb on + strand, within intS at 2465.083 kb on - strand, within intS at 2465.083 kb on - strand, within intS at 2465.085 kb on + strand, within intS at 2465.085 kb on + strand, within intS
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 2,462,333 - -0.2 2,462,336 - -1.1 2,462,344 - -0.0 2,462,344 - +0.2 2,462,394 - vacJ b2346 0.16 -0.1 2,462,399 - vacJ b2346 0.17 -2.0 2,462,403 - vacJ b2346 0.17 -1.3 2,462,405 - vacJ b2346 0.17 -0.9 2,462,406 - vacJ b2346 0.17 -1.3 2,462,421 - vacJ b2346 0.19 -0.8 2,462,438 - vacJ b2346 0.22 +0.3 2,462,447 + vacJ b2346 0.23 +0.8 2,462,465 + vacJ b2346 0.25 +0.8 2,462,471 - vacJ b2346 0.26 +0.6 2,462,491 - vacJ b2346 0.29 -0.1 2,462,507 - vacJ b2346 0.31 -0.3 2,462,619 + vacJ b2346 0.46 -1.5 2,462,619 + vacJ b2346 0.46 -1.2 2,462,623 + vacJ b2346 0.46 +0.2 2,462,708 - vacJ b2346 0.57 -0.4 2,462,799 - vacJ b2346 0.69 +0.3 2,462,878 + vacJ b2346 0.80 +0.0 2,462,909 + vacJ b2346 0.84 +0.8 2,462,913 - vacJ b2346 0.85 -0.7 2,463,052 + -1.4 2,463,052 + -0.5 2,463,126 + -0.0 2,463,170 + -0.9 2,463,186 + -0.0 2,463,194 - -2.2 2,463,365 - +0.1 2,463,415 - -0.8 2,463,431 - yfdC b2347 0.12 -1.7 2,463,473 + yfdC b2347 0.16 -0.6 2,463,568 - yfdC b2347 0.26 +1.5 2,463,568 - yfdC b2347 0.26 -1.6 2,463,648 - yfdC b2347 0.35 -0.4 2,463,683 + yfdC b2347 0.39 +1.8 2,463,728 + yfdC b2347 0.43 +0.0 2,463,736 + yfdC b2347 0.44 -0.8 2,463,736 + yfdC b2347 0.44 -0.8 2,463,777 + yfdC b2347 0.49 -0.2 2,463,807 - yfdC b2347 0.52 -0.8 2,463,807 - yfdC b2347 0.52 -1.5 2,463,811 + yfdC b2347 0.52 -0.1 2,463,855 - yfdC b2347 0.57 -0.3 2,463,858 + yfdC b2347 0.57 -2.0 2,463,858 + yfdC b2347 0.57 -0.4 2,463,861 - yfdC b2347 0.58 +2.4 2,463,861 - yfdC b2347 0.58 -1.5 2,463,868 - yfdC b2347 0.58 +0.2 2,463,926 - yfdC b2347 0.65 -1.1 2,463,980 + yfdC b2347 0.70 -0.8 2,463,984 + yfdC b2347 0.71 +0.8 2,463,997 + yfdC b2347 0.72 -0.0 2,464,005 - yfdC b2347 0.73 +0.4 2,464,005 - yfdC b2347 0.73 +0.8 2,464,007 + yfdC b2347 0.73 -0.1 2,464,059 + yfdC b2347 0.79 -0.5 2,464,059 - yfdC b2347 0.79 +0.8 2,464,094 - yfdC b2347 0.83 +0.2 2,464,127 + yfdC b2347 0.86 -0.5 2,464,149 + yfdC b2347 0.89 -0.0 2,464,177 - -0.1 2,464,312 + +1.8 2,464,312 + +0.4 2,464,320 - -0.5 2,464,427 + -0.8 2,464,515 + +0.2 2,464,592 - -0.2 2,464,644 - -0.2 2,464,644 - -0.2 2,464,646 + +0.3 2,464,764 + intS b2349 0.17 +0.4 2,464,772 - intS b2349 0.18 -0.0 2,464,813 - intS b2349 0.21 -0.4 2,464,875 + intS b2349 0.27 -0.0 2,464,875 + intS b2349 0.27 +0.1 2,465,006 + intS b2349 0.38 +0.1 2,465,083 - intS b2349 0.45 +0.1 2,465,083 - intS b2349 0.45 -1.4 2,465,085 + intS b2349 0.45 -0.3 2,465,085 + intS b2349 0.45 +0.8
Or see this region's nucleotide sequence