Strain Fitness in Escherichia coli BW25113 around b2264

Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmenB and yfbB are separated by 14 nucleotidesyfbB and menD overlap by 4 nucleotidesmenD and menF are separated by 88 nucleotides b2262: menB - naphthoate synthase (NCBI), at 2,373,984 to 2,374,841 menB b2263: yfbB - predicted peptidase (NCBI), at 2,374,856 to 2,375,614 yfbB b2264: menD - 2-succinyl-6-hydroxy-2, 4-cyclohexadiene-1-carboxylate synthase (NCBI), at 2,375,611 to 2,377,281 menD b2265: menF - isochorismate hydroxymutase 2, menaquinone biosynthesis (VIMSS), at 2,377,370 to 2,378,665 menF Position (kb) 2375 2376 2377 2378Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 2374.673 kb on - strand, within menBat 2374.831 kb on + strandat 2374.831 kb on + strandat 2374.885 kb on + strandat 2374.893 kb on - strandat 2375.223 kb on - strand, within yfbBat 2375.307 kb on - strand, within yfbBat 2375.363 kb on - strand, within yfbBat 2375.570 kb on + strandat 2375.570 kb on + strandat 2375.616 kb on - strandat 2375.616 kb on - strandat 2375.795 kb on - strand, within menDat 2376.112 kb on - strand, within menDat 2376.143 kb on - strand, within menDat 2376.143 kb on - strand, within menDat 2376.359 kb on + strand, within menDat 2376.359 kb on + strand, within menDat 2376.515 kb on + strand, within menDat 2376.554 kb on - strand, within menDat 2376.585 kb on + strand, within menDat 2376.585 kb on + strand, within menDat 2376.605 kb on + strand, within menDat 2376.790 kb on + strand, within menDat 2376.798 kb on - strand, within menDat 2376.798 kb on - strand, within menDat 2377.105 kb on + strand, within menDat 2377.105 kb on + strand, within menDat 2377.151 kb on - strandat 2377.217 kb on - strandat 2377.217 kb on - strandat 2377.232 kb on - strandat 2377.294 kb on + strandat 2377.312 kb on + strandat 2377.320 kb on - strandat 2377.320 kb on - strandat 2377.320 kb on - strandat 2377.398 kb on + strandat 2377.451 kb on + strandat 2377.531 kb on + strand, within menFat 2377.590 kb on - strand, within menFat 2377.590 kb on - strand, within menFat 2377.662 kb on - strand, within menFat 2377.692 kb on + strand, within menFat 2377.692 kb on + strand, within menFat 2377.692 kb on + strand, within menFat 2377.814 kb on - strand, within menFat 2377.851 kb on + strand, within menFat 2377.901 kb on - strand, within menFat 2378.222 kb on + strand, within menFat 2378.224 kb on + strand, within menFat 2378.261 kb on + strand, within menFat 2378.269 kb on - strand, within menFat 2378.269 kb on - strand, within menFat 2378.269 kb on - strand, within menFat 2378.269 kb on - strand, within menFat 2378.269 kb on - strand, within menF

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM
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2,374,673 - menB b2262 0.80 -1.4
2,374,831 + -1.1
2,374,831 + -4.1
2,374,885 + -0.5
2,374,893 - -0.4
2,375,223 - yfbB b2263 0.48 -0.6
2,375,307 - yfbB b2263 0.59 -0.6
2,375,363 - yfbB b2263 0.67 -1.3
2,375,570 + -1.4
2,375,570 + +0.6
2,375,616 - +0.8
2,375,616 - -0.2
2,375,795 - menD b2264 0.11 -0.5
2,376,112 - menD b2264 0.30 -0.1
2,376,143 - menD b2264 0.32 -1.2
2,376,143 - menD b2264 0.32 -1.2
2,376,359 + menD b2264 0.45 +0.1
2,376,359 + menD b2264 0.45 -0.2
2,376,515 + menD b2264 0.54 -0.8
2,376,554 - menD b2264 0.56 +2.4
2,376,585 + menD b2264 0.58 -1.4
2,376,585 + menD b2264 0.58 -1.7
2,376,605 + menD b2264 0.59 -1.4
2,376,790 + menD b2264 0.71 -2.3
2,376,798 - menD b2264 0.71 -2.1
2,376,798 - menD b2264 0.71 -0.4
2,377,105 + menD b2264 0.89 -0.4
2,377,105 + menD b2264 0.89 -0.6
2,377,151 - -1.0
2,377,217 - -1.9
2,377,217 - -3.0
2,377,232 - +0.4
2,377,294 + +0.8
2,377,312 + +0.1
2,377,320 - +0.9
2,377,320 - +0.9
2,377,320 - -0.2
2,377,398 + +0.2
2,377,451 + -1.1
2,377,531 + menF b2265 0.12 +0.2
2,377,590 - menF b2265 0.17 -0.1
2,377,590 - menF b2265 0.17 -0.9
2,377,662 - menF b2265 0.23 +0.5
2,377,692 + menF b2265 0.25 -0.8
2,377,692 + menF b2265 0.25 -0.4
2,377,692 + menF b2265 0.25 +0.4
2,377,814 - menF b2265 0.34 +0.8
2,377,851 + menF b2265 0.37 -0.8
2,377,901 - menF b2265 0.41 +1.9
2,378,222 + menF b2265 0.66 +0.5
2,378,224 + menF b2265 0.66 -1.0
2,378,261 + menF b2265 0.69 -0.7
2,378,269 - menF b2265 0.69 +0.2
2,378,269 - menF b2265 0.69 +0.5
2,378,269 - menF b2265 0.69 -0.4
2,378,269 - menF b2265 0.69 +1.4
2,378,269 - menF b2265 0.69 +1.5

Or see this region's nucleotide sequence