Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt glpB and glpC overlap by 4 nucleotides glpC and yfaD are separated by 192 nucleotides
b2242: glpB - anaerobic glycerol-3-phosphate dehydrogenase subunit B (NCBI), at 2,352,287 to 2,353,546
glpB
b2243: glpC - sn-glycerol-3-phosphate dehydrogenase (anaerobic), small subunit (NCBI), at 2,353,543 to 2,354,733
glpC
b2244: yfaD - hypothetical protein (NCBI), at 2,354,926 to 2,355,825
yfaD
Position (kb)
2353
2354
2355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2352.547 kb on + strand, within glpB at 2352.631 kb on + strand, within glpB at 2352.650 kb on + strand, within glpB at 2352.650 kb on + strand, within glpB at 2352.787 kb on - strand, within glpB at 2353.281 kb on + strand, within glpB at 2353.291 kb on + strand, within glpB at 2353.291 kb on + strand, within glpB at 2353.326 kb on - strand, within glpB at 2353.326 kb on - strand, within glpB at 2353.379 kb on - strand, within glpB at 2353.400 kb on + strand, within glpB at 2353.400 kb on + strand, within glpB at 2353.436 kb on + strand at 2353.436 kb on + strand at 2353.446 kb on + strand at 2353.575 kb on - strand at 2353.575 kb on - strand at 2353.577 kb on + strand at 2353.577 kb on + strand at 2353.615 kb on - strand at 2353.684 kb on + strand, within glpC at 2353.686 kb on + strand, within glpC at 2353.696 kb on - strand, within glpC at 2353.696 kb on - strand, within glpC at 2353.767 kb on + strand, within glpC at 2353.812 kb on + strand, within glpC at 2353.853 kb on + strand, within glpC at 2353.853 kb on + strand, within glpC at 2353.871 kb on - strand, within glpC at 2354.115 kb on - strand, within glpC at 2354.125 kb on - strand, within glpC at 2354.125 kb on - strand, within glpC at 2354.169 kb on + strand, within glpC at 2354.278 kb on - strand, within glpC at 2354.438 kb on - strand, within glpC at 2354.534 kb on + strand, within glpC at 2354.542 kb on - strand, within glpC at 2354.591 kb on + strand, within glpC at 2354.676 kb on + strand at 2354.676 kb on + strand at 2354.684 kb on - strand at 2354.752 kb on + strand at 2354.793 kb on - strand at 2354.793 kb on - strand at 2354.892 kb on - strand at 2354.926 kb on - strand at 2354.926 kb on - strand at 2354.941 kb on - strand at 2354.941 kb on - strand at 2355.020 kb on + strand, within yfaD at 2355.149 kb on + strand, within yfaD at 2355.322 kb on - strand, within yfaD at 2355.322 kb on - strand, within yfaD at 2355.322 kb on - strand, within yfaD at 2355.369 kb on + strand, within yfaD at 2355.396 kb on + strand, within yfaD at 2355.438 kb on + strand, within yfaD at 2355.438 kb on + strand, within yfaD at 2355.448 kb on + strand, within yfaD at 2355.448 kb on + strand, within yfaD at 2355.448 kb on + strand, within yfaD at 2355.499 kb on + strand, within yfaD at 2355.665 kb on - strand, within yfaD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 2,352,547 + glpB b2242 0.21 -2.0 2,352,631 + glpB b2242 0.27 -0.9 2,352,650 + glpB b2242 0.29 -1.8 2,352,650 + glpB b2242 0.29 -2.2 2,352,787 - glpB b2242 0.40 -0.1 2,353,281 + glpB b2242 0.79 -2.7 2,353,291 + glpB b2242 0.80 -2.8 2,353,291 + glpB b2242 0.80 +0.8 2,353,326 - glpB b2242 0.82 -0.4 2,353,326 - glpB b2242 0.82 +1.8 2,353,379 - glpB b2242 0.87 +0.1 2,353,400 + glpB b2242 0.88 -0.5 2,353,400 + glpB b2242 0.88 -0.2 2,353,436 + -0.8 2,353,436 + -1.0 2,353,446 + -1.8 2,353,575 - +2.8 2,353,575 - +0.1 2,353,577 + +0.7 2,353,577 + -2.4 2,353,615 - +0.4 2,353,684 + glpC b2243 0.12 -0.6 2,353,686 + glpC b2243 0.12 -1.8 2,353,696 - glpC b2243 0.13 -0.8 2,353,696 - glpC b2243 0.13 -2.6 2,353,767 + glpC b2243 0.19 -0.4 2,353,812 + glpC b2243 0.23 -1.2 2,353,853 + glpC b2243 0.26 -1.3 2,353,853 + glpC b2243 0.26 -2.2 2,353,871 - glpC b2243 0.28 -1.2 2,354,115 - glpC b2243 0.48 -1.7 2,354,125 - glpC b2243 0.49 +0.8 2,354,125 - glpC b2243 0.49 -1.5 2,354,169 + glpC b2243 0.53 -0.9 2,354,278 - glpC b2243 0.62 -3.0 2,354,438 - glpC b2243 0.75 -0.3 2,354,534 + glpC b2243 0.83 -1.8 2,354,542 - glpC b2243 0.84 -0.2 2,354,591 + glpC b2243 0.88 -1.2 2,354,676 + -0.1 2,354,676 + -1.0 2,354,684 - -3.3 2,354,752 + +1.7 2,354,793 - +0.5 2,354,793 - +0.4 2,354,892 - +1.2 2,354,926 - -0.5 2,354,926 - -1.0 2,354,941 - -0.2 2,354,941 - -0.8 2,355,020 + yfaD b2244 0.10 -1.2 2,355,149 + yfaD b2244 0.25 +0.6 2,355,322 - yfaD b2244 0.44 -0.8 2,355,322 - yfaD b2244 0.44 -1.3 2,355,322 - yfaD b2244 0.44 +0.3 2,355,369 + yfaD b2244 0.49 -0.1 2,355,396 + yfaD b2244 0.52 -0.3 2,355,438 + yfaD b2244 0.57 +0.1 2,355,438 + yfaD b2244 0.57 -0.8 2,355,448 + yfaD b2244 0.58 +0.2 2,355,448 + yfaD b2244 0.58 -0.1 2,355,448 + yfaD b2244 0.58 +0.5 2,355,499 + yfaD b2244 0.64 +0.8 2,355,665 - yfaD b2244 0.82 -0.9
Or see this region's nucleotide sequence