Strain Fitness in Escherichia coli BW25113 around b2121

Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyehM and yehP overlap by 8 nucleotidesyehP and yehQ overlap by 4 nucleotides b2120: yehM - hypothetical protein (NCBI), at 2,203,717 to 2,205,996 yehM b2121: yehP - hypothetical protein (NCBI), at 2,205,989 to 2,207,125 yehP b2122: yehQ - orf, hypothetical protein (VIMSS), at 2,207,122 to 2,209,122 yehQ Position (kb) 2205 2206 2207 2208Strain fitness (log2 ratio) -3 -2 -1 0 1at 2205.010 kb on - strand, within yehMat 2205.376 kb on + strand, within yehMat 2205.376 kb on + strand, within yehMat 2205.376 kb on + strand, within yehMat 2205.384 kb on + strand, within yehMat 2205.384 kb on + strand, within yehMat 2205.492 kb on - strand, within yehMat 2205.492 kb on - strand, within yehMat 2205.625 kb on + strand, within yehMat 2205.802 kb on + strandat 2205.919 kb on - strandat 2205.958 kb on + strandat 2206.144 kb on + strand, within yehPat 2206.144 kb on - strand, within yehPat 2206.144 kb on - strand, within yehPat 2206.189 kb on + strand, within yehPat 2206.202 kb on - strand, within yehPat 2206.257 kb on - strand, within yehPat 2206.257 kb on - strand, within yehPat 2206.296 kb on - strand, within yehPat 2206.390 kb on - strand, within yehPat 2206.390 kb on - strand, within yehPat 2206.547 kb on + strand, within yehPat 2206.547 kb on + strand, within yehPat 2206.609 kb on + strand, within yehPat 2206.686 kb on + strand, within yehPat 2206.776 kb on - strand, within yehPat 2206.809 kb on + strand, within yehPat 2206.809 kb on + strand, within yehPat 2206.824 kb on - strand, within yehPat 2206.855 kb on - strand, within yehPat 2207.171 kb on - strandat 2207.171 kb on - strandat 2207.253 kb on - strandat 2207.469 kb on + strand, within yehQat 2207.500 kb on - strand, within yehQat 2207.524 kb on + strand, within yehQat 2207.629 kb on + strand, within yehQat 2207.682 kb on - strand, within yehQat 2207.949 kb on - strand, within yehQat 2208.004 kb on + strand, within yehQat 2208.048 kb on + strand, within yehQat 2208.088 kb on + strand, within yehQat 2208.096 kb on - strand, within yehQ

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM
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2,205,010 - yehM b2120 0.57 -2.1
2,205,376 + yehM b2120 0.73 -1.5
2,205,376 + yehM b2120 0.73 +0.5
2,205,376 + yehM b2120 0.73 -0.0
2,205,384 + yehM b2120 0.73 -0.6
2,205,384 + yehM b2120 0.73 -0.2
2,205,492 - yehM b2120 0.78 +0.2
2,205,492 - yehM b2120 0.78 +0.9
2,205,625 + yehM b2120 0.84 -0.5
2,205,802 + +1.2
2,205,919 - +0.5
2,205,958 + +0.1
2,206,144 + yehP b2121 0.14 -1.4
2,206,144 - yehP b2121 0.14 -1.5
2,206,144 - yehP b2121 0.14 +0.4
2,206,189 + yehP b2121 0.18 -1.3
2,206,202 - yehP b2121 0.19 +0.2
2,206,257 - yehP b2121 0.24 -0.2
2,206,257 - yehP b2121 0.24 +0.1
2,206,296 - yehP b2121 0.27 -0.4
2,206,390 - yehP b2121 0.35 -0.2
2,206,390 - yehP b2121 0.35 -1.8
2,206,547 + yehP b2121 0.49 -0.1
2,206,547 + yehP b2121 0.49 -2.3
2,206,609 + yehP b2121 0.55 -1.8
2,206,686 + yehP b2121 0.61 +0.5
2,206,776 - yehP b2121 0.69 +0.8
2,206,809 + yehP b2121 0.72 -2.9
2,206,809 + yehP b2121 0.72 -2.0
2,206,824 - yehP b2121 0.73 -1.3
2,206,855 - yehP b2121 0.76 -0.1
2,207,171 - +0.6
2,207,171 - +0.7
2,207,253 - -0.6
2,207,469 + yehQ b2122 0.17 +0.6
2,207,500 - yehQ b2122 0.19 -1.1
2,207,524 + yehQ b2122 0.20 +0.1
2,207,629 + yehQ b2122 0.25 +0.2
2,207,682 - yehQ b2122 0.28 -0.7
2,207,949 - yehQ b2122 0.41 -0.6
2,208,004 + yehQ b2122 0.44 +0.2
2,208,048 + yehQ b2122 0.46 +0.1
2,208,088 + yehQ b2122 0.48 -0.1
2,208,096 - yehQ b2122 0.49 +0.1

Or see this region's nucleotide sequence