Strain Fitness in Escherichia coli BW25113 around b1611

Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttus and fumC overlap by 4 nucleotidesfumC and fumA are separated by 142 nucleotides b1610: tus - DNA replication terminus site-binding protein (NCBI), at 1,682,283 to 1,683,212 tus b1611: fumC - fumarate hydratase (NCBI), at 1,683,209 to 1,684,612 fumC b1612: fumA - fumarate hydratase (fumarase A), aerobic Class I (NCBI), at 1,684,755 to 1,686,401 fumA Position (kb) 1683 1684 1685Strain fitness (log2 ratio) -2 -1 0 1 2at 1682.223 kb on + strandat 1682.223 kb on + strandat 1682.306 kb on - strandat 1682.451 kb on - strand, within tusat 1682.567 kb on - strand, within tusat 1682.620 kb on + strand, within tusat 1682.620 kb on + strand, within tusat 1682.820 kb on + strand, within tusat 1682.838 kb on + strand, within tusat 1682.878 kb on + strand, within tusat 1682.886 kb on - strand, within tusat 1682.941 kb on - strand, within tusat 1682.941 kb on - strand, within tusat 1683.046 kb on + strand, within tusat 1683.046 kb on + strand, within tusat 1683.207 kb on + strandat 1683.241 kb on + strandat 1683.295 kb on + strandat 1683.295 kb on + strandat 1683.439 kb on + strand, within fumCat 1683.595 kb on + strand, within fumCat 1683.612 kb on + strand, within fumCat 1683.612 kb on + strand, within fumCat 1683.665 kb on - strand, within fumCat 1683.665 kb on - strand, within fumCat 1683.709 kb on + strand, within fumCat 1683.709 kb on + strand, within fumCat 1683.859 kb on - strand, within fumCat 1683.859 kb on - strand, within fumCat 1683.981 kb on - strand, within fumCat 1684.016 kb on - strand, within fumCat 1684.016 kb on - strand, within fumCat 1684.247 kb on + strand, within fumCat 1684.247 kb on + strand, within fumCat 1684.600 kb on + strandat 1684.600 kb on + strandat 1685.163 kb on + strand, within fumAat 1685.193 kb on + strand, within fumAat 1685.290 kb on - strand, within fumAat 1685.290 kb on - strand, within fumAat 1685.310 kb on + strand, within fumAat 1685.344 kb on + strand, within fumA

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM
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1,682,223 + -0.9
1,682,223 + +0.7
1,682,306 - -1.3
1,682,451 - tus b1610 0.18 -0.6
1,682,567 - tus b1610 0.31 -0.9
1,682,620 + tus b1610 0.36 +0.7
1,682,620 + tus b1610 0.36 -1.5
1,682,820 + tus b1610 0.58 +0.9
1,682,838 + tus b1610 0.60 -1.7
1,682,878 + tus b1610 0.64 -0.0
1,682,886 - tus b1610 0.65 -0.9
1,682,941 - tus b1610 0.71 +0.3
1,682,941 - tus b1610 0.71 +0.3
1,683,046 + tus b1610 0.82 +1.9
1,683,046 + tus b1610 0.82 +1.0
1,683,207 + -2.0
1,683,241 + +0.7
1,683,295 + +0.3
1,683,295 + +1.4
1,683,439 + fumC b1611 0.16 +0.3
1,683,595 + fumC b1611 0.27 +0.6
1,683,612 + fumC b1611 0.29 -0.0
1,683,612 + fumC b1611 0.29 -0.6
1,683,665 - fumC b1611 0.32 +1.5
1,683,665 - fumC b1611 0.32 -0.7
1,683,709 + fumC b1611 0.36 -1.3
1,683,709 + fumC b1611 0.36 +1.7
1,683,859 - fumC b1611 0.46 +0.5
1,683,859 - fumC b1611 0.46 +0.7
1,683,981 - fumC b1611 0.55 +0.9
1,684,016 - fumC b1611 0.57 +0.2
1,684,016 - fumC b1611 0.57 -0.0
1,684,247 + fumC b1611 0.74 +1.1
1,684,247 + fumC b1611 0.74 -0.3
1,684,600 + +0.1
1,684,600 + -0.8
1,685,163 + fumA b1612 0.25 -0.9
1,685,193 + fumA b1612 0.27 +0.7
1,685,290 - fumA b1612 0.32 +1.5
1,685,290 - fumA b1612 0.32 +1.2
1,685,310 + fumA b1612 0.34 -0.0
1,685,344 + fumA b1612 0.36 -0.3

Or see this region's nucleotide sequence