Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ycaN and ycaK are separated by 99 nucleotides ycaK and pflA are separated by 81 nucleotides pflA and pflB are separated by 191 nucleotides
b0900: ycaN - predicted DNA-binding transcriptional regulator (NCBI), at 947,883 to 948,791
ycaN
b0901: ycaK - hypothetical protein (NCBI), at 948,891 to 949,481
ycaK
b0902: pflA - pyruvate formate lyase activating enzyme 1 (NCBI), at 949,563 to 950,303
pflA
b0903: pflB - pyruvate formate lyase I (NCBI), at 950,495 to 952,777
pflB
Position (kb) 949
950
951 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2
3 at 948.565 kb on - strand, within ycaN at 948.616 kb on + strand, within ycaN at 948.616 kb on + strand, within ycaN at 948.616 kb on + strand, within ycaN at 948.632 kb on - strand, within ycaN at 948.673 kb on + strand, within ycaN at 948.702 kb on + strand at 948.706 kb on + strand at 948.707 kb on + strand at 948.707 kb on + strand at 948.707 kb on + strand at 948.707 kb on + strand at 948.708 kb on + strand at 948.708 kb on + strand at 948.708 kb on + strand at 948.708 kb on + strand at 948.714 kb on + strand at 948.715 kb on - strand at 948.715 kb on - strand at 948.715 kb on - strand at 948.715 kb on - strand at 948.716 kb on - strand at 948.716 kb on - strand at 948.716 kb on - strand at 948.716 kb on - strand at 948.722 kb on - strand at 948.722 kb on - strand at 948.725 kb on + strand at 948.752 kb on + strand at 948.752 kb on + strand at 948.773 kb on + strand at 948.775 kb on + strand at 948.837 kb on + strand at 948.837 kb on + strand at 948.837 kb on + strand at 948.838 kb on - strand at 948.839 kb on + strand at 948.839 kb on + strand at 948.845 kb on - strand at 948.845 kb on - strand at 948.850 kb on + strand at 948.850 kb on + strand at 948.851 kb on + strand at 948.851 kb on + strand at 948.852 kb on + strand at 948.858 kb on - strand at 948.863 kb on + strand at 948.863 kb on + strand at 948.863 kb on + strand at 948.863 kb on + strand at 948.863 kb on + strand at 948.863 kb on + strand at 948.863 kb on + strand at 948.871 kb on - strand at 948.920 kb on + strand at 948.920 kb on + strand at 948.920 kb on + strand at 948.920 kb on + strand at 948.920 kb on + strand at 948.920 kb on + strand at 948.920 kb on + strand at 948.921 kb on + strand at 948.921 kb on + strand at 948.923 kb on - strand at 948.928 kb on - strand at 948.928 kb on - strand at 948.928 kb on - strand at 948.928 kb on - strand at 948.930 kb on + strand at 948.930 kb on + strand at 948.930 kb on + strand at 948.931 kb on + strand at 948.938 kb on + strand at 948.938 kb on - strand at 948.938 kb on - strand at 948.946 kb on + strand at 948.949 kb on + strand at 948.949 kb on + strand at 948.951 kb on + strand, within ycaK at 948.951 kb on + strand, within ycaK at 948.962 kb on + strand, within ycaK at 948.964 kb on + strand, within ycaK at 948.966 kb on + strand, within ycaK at 948.966 kb on + strand, within ycaK at 948.976 kb on + strand, within ycaK at 948.988 kb on + strand, within ycaK at 949.012 kb on + strand, within ycaK at 949.030 kb on - strand, within ycaK at 949.045 kb on + strand, within ycaK at 949.046 kb on - strand, within ycaK at 949.050 kb on + strand, within ycaK at 949.052 kb on + strand, within ycaK at 949.052 kb on - strand, within ycaK at 949.060 kb on - strand, within ycaK at 949.107 kb on + strand, within ycaK at 949.107 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.111 kb on + strand, within ycaK at 949.119 kb on - strand, within ycaK at 949.121 kb on + strand, within ycaK at 949.128 kb on - strand, within ycaK at 949.143 kb on - strand, within ycaK at 949.143 kb on - strand, within ycaK at 949.157 kb on + strand, within ycaK at 949.161 kb on - strand, within ycaK at 949.161 kb on - strand, within ycaK at 949.168 kb on + strand, within ycaK at 949.169 kb on + strand, within ycaK at 949.179 kb on + strand, within ycaK at 949.179 kb on + strand, within ycaK at 949.187 kb on - strand, within ycaK at 949.203 kb on + strand, within ycaK at 949.203 kb on + strand, within ycaK at 949.220 kb on + strand, within ycaK at 949.220 kb on + strand, within ycaK at 949.221 kb on + strand, within ycaK at 949.221 kb on + strand, within ycaK at 949.222 kb on + strand, within ycaK at 949.222 kb on + strand, within ycaK at 949.228 kb on - strand, within ycaK at 949.255 kb on - strand, within ycaK at 949.269 kb on + strand, within ycaK at 949.269 kb on + strand, within ycaK at 949.269 kb on + strand, within ycaK at 949.274 kb on + strand, within ycaK at 949.283 kb on + strand, within ycaK at 949.283 kb on + strand, within ycaK at 949.299 kb on + strand, within ycaK at 949.388 kb on + strand, within ycaK at 949.388 kb on + strand, within ycaK at 949.388 kb on + strand, within ycaK at 949.393 kb on + strand, within ycaK at 949.393 kb on + strand, within ycaK at 949.396 kb on + strand, within ycaK at 949.396 kb on + strand, within ycaK at 949.396 kb on + strand, within ycaK at 949.396 kb on - strand, within ycaK at 949.447 kb on - strand at 949.460 kb on + strand at 949.460 kb on + strand at 949.460 kb on + strand at 949.466 kb on + strand at 949.468 kb on - strand at 949.468 kb on - strand at 949.473 kb on + strand at 949.476 kb on + strand at 949.525 kb on - strand at 949.587 kb on - strand at 949.587 kb on - strand at 949.597 kb on - strand at 949.610 kb on - strand at 949.611 kb on + strand at 949.615 kb on - strand at 949.642 kb on - strand, within pflA at 949.642 kb on - strand, within pflA at 949.648 kb on + strand, within pflA at 949.664 kb on - strand, within pflA at 949.675 kb on - strand, within pflA at 949.685 kb on - strand, within pflA at 949.799 kb on - strand, within pflA at 949.860 kb on - strand, within pflA at 949.863 kb on - strand, within pflA at 949.870 kb on - strand, within pflA at 949.997 kb on - strand, within pflA at 949.998 kb on + strand, within pflA at 950.126 kb on - strand, within pflA at 950.126 kb on - strand, within pflA at 950.270 kb on + strand at 950.270 kb on + strand at 950.511 kb on + strand at 950.520 kb on - strand at 950.571 kb on - strand at 950.686 kb on + strand at 950.686 kb on + strand at 950.707 kb on + strand at 950.707 kb on + strand at 950.722 kb on + strand at 950.722 kb on + strand at 950.772 kb on - strand, within pflB at 950.772 kb on - strand, within pflB at 950.777 kb on + strand, within pflB at 950.836 kb on - strand, within pflB at 950.836 kb on - strand, within pflB at 950.854 kb on + strand, within pflB at 950.944 kb on - strand, within pflB at 950.974 kb on - strand, within pflB at 950.974 kb on - strand, within pflB at 950.975 kb on + strand, within pflB at 951.040 kb on + strand, within pflB at 951.040 kb on + strand, within pflB at 951.120 kb on - strand, within pflB at 951.193 kb on - strand, within pflB at 951.269 kb on + strand, within pflB at 951.269 kb on + strand, within pflB at 951.281 kb on + strand, within pflB at 951.288 kb on + strand, within pflB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 948,565 - ycaN b0900 0.75 -0.3 948,616 + ycaN b0900 0.81 +0.7 948,616 + ycaN b0900 0.81 +0.7 948,616 + ycaN b0900 0.81 -0.3 948,632 - ycaN b0900 0.82 +0.7 948,673 + ycaN b0900 0.87 -1.7 948,702 + +0.7 948,706 + +0.7 948,707 + -0.3 948,707 + +0.7 948,707 + +0.7 948,707 + +0.7 948,708 + +0.7 948,708 + +0.7 948,708 + +0.7 948,708 + +0.7 948,714 + -0.3 948,715 - +0.7 948,715 - +0.7 948,715 - +2.3 948,715 - +0.7 948,716 - +1.5 948,716 - -0.9 948,716 - +2.3 948,716 - -0.3 948,722 - -0.3 948,722 - +3.7 948,725 + +0.1 948,752 + +0.7 948,752 + -0.3 948,773 + +1.0 948,775 + -0.9 948,837 + -2.1 948,837 + -1.5 948,837 + -0.6 948,838 - +0.7 948,839 + +0.8 948,839 + +0.4 948,845 - -0.3 948,845 - +0.7 948,850 + +0.5 948,850 + -0.3 948,851 + -2.6 948,851 + +0.7 948,852 + -0.4 948,858 - +0.1 948,863 + +0.1 948,863 + +1.1 948,863 + -1.5 948,863 + +0.0 948,863 + -0.6 948,863 + -1.3 948,863 + -0.0 948,871 - +0.7 948,920 + +1.2 948,920 + -0.3 948,920 + +0.2 948,920 + -1.5 948,920 + +0.6 948,920 + -1.0 948,920 + +1.3 948,921 + +0.2 948,921 + -0.7 948,923 - -0.3 948,928 - +1.3 948,928 - -0.9 948,928 - -0.9 948,928 - -0.3 948,930 + -0.7 948,930 + +0.9 948,930 + -0.0 948,931 + +0.5 948,938 + +1.1 948,938 - -0.3 948,938 - -0.9 948,946 + -0.6 948,949 + +0.1 948,949 + +0.7 948,951 + ycaK b0901 0.10 +0.4 948,951 + ycaK b0901 0.10 -0.3 948,962 + ycaK b0901 0.12 +0.5 948,964 + ycaK b0901 0.12 -0.3 948,966 + ycaK b0901 0.13 -0.4 948,966 + ycaK b0901 0.13 -0.3 948,976 + ycaK b0901 0.14 +0.7 948,988 + ycaK b0901 0.16 +0.1 949,012 + ycaK b0901 0.20 +1.6 949,030 - ycaK b0901 0.24 +0.7 949,045 + ycaK b0901 0.26 +1.9 949,046 - ycaK b0901 0.26 -0.3 949,050 + ycaK b0901 0.27 -0.9 949,052 + ycaK b0901 0.27 +1.1 949,052 - ycaK b0901 0.27 -0.3 949,060 - ycaK b0901 0.29 +0.7 949,107 + ycaK b0901 0.37 +0.1 949,107 + ycaK b0901 0.37 +0.7 949,111 + ycaK b0901 0.37 +0.9 949,111 + ycaK b0901 0.37 -1.9 949,111 + ycaK b0901 0.37 +0.7 949,111 + ycaK b0901 0.37 -2.4 949,111 + ycaK b0901 0.37 +0.7 949,111 + ycaK b0901 0.37 +0.3 949,111 + ycaK b0901 0.37 -1.6 949,111 + ycaK b0901 0.37 +0.7 949,119 - ycaK b0901 0.39 +1.0 949,121 + ycaK b0901 0.39 -0.6 949,128 - ycaK b0901 0.40 -0.6 949,143 - ycaK b0901 0.43 +0.7 949,143 - ycaK b0901 0.43 -0.9 949,157 + ycaK b0901 0.45 -1.0 949,161 - ycaK b0901 0.46 -0.3 949,161 - ycaK b0901 0.46 +0.7 949,168 + ycaK b0901 0.47 -0.1 949,169 + ycaK b0901 0.47 +0.1 949,179 + ycaK b0901 0.49 +0.6 949,179 + ycaK b0901 0.49 -0.9 949,187 - ycaK b0901 0.50 -0.3 949,203 + ycaK b0901 0.53 -1.6 949,203 + ycaK b0901 0.53 -1.3 949,220 + ycaK b0901 0.56 -1.5 949,220 + ycaK b0901 0.56 +0.1 949,221 + ycaK b0901 0.56 +0.1 949,221 + ycaK b0901 0.56 +0.4 949,222 + ycaK b0901 0.56 +1.5 949,222 + ycaK b0901 0.56 -0.6 949,228 - ycaK b0901 0.57 +0.7 949,255 - ycaK b0901 0.62 +0.7 949,269 + ycaK b0901 0.64 +1.3 949,269 + ycaK b0901 0.64 -1.1 949,269 + ycaK b0901 0.64 -1.7 949,274 + ycaK b0901 0.65 -0.3 949,283 + ycaK b0901 0.66 +0.5 949,283 + ycaK b0901 0.66 -0.6 949,299 + ycaK b0901 0.69 +1.0 949,388 + ycaK b0901 0.84 -2.1 949,388 + ycaK b0901 0.84 +0.7 949,388 + ycaK b0901 0.84 +1.2 949,393 + ycaK b0901 0.85 +0.1 949,393 + ycaK b0901 0.85 -1.3 949,396 + ycaK b0901 0.85 +0.7 949,396 + ycaK b0901 0.85 -1.3 949,396 + ycaK b0901 0.85 -0.5 949,396 - ycaK b0901 0.85 +0.7 949,447 - +0.7 949,460 + -1.0 949,460 + +0.7 949,460 + +1.7 949,466 + +0.7 949,468 - +0.7 949,468 - +0.7 949,473 + +1.7 949,476 + -1.4 949,525 - +0.7 949,587 - -1.9 949,587 - -1.5 949,597 - -0.8 949,610 - -3.2 949,611 + +0.7 949,615 - -1.3 949,642 - pflA b0902 0.11 -4.7 949,642 - pflA b0902 0.11 -3.0 949,648 + pflA b0902 0.11 +0.7 949,664 - pflA b0902 0.14 -1.9 949,675 - pflA b0902 0.15 -3.4 949,685 - pflA b0902 0.16 -3.4 949,799 - pflA b0902 0.32 -4.1 949,860 - pflA b0902 0.40 -3.8 949,863 - pflA b0902 0.40 -5.6 949,870 - pflA b0902 0.41 -3.5 949,997 - pflA b0902 0.59 -2.3 949,998 + pflA b0902 0.59 -2.9 950,126 - pflA b0902 0.76 -3.1 950,126 - pflA b0902 0.76 +0.7 950,270 + -3.2 950,270 + -1.7 950,511 + -1.6 950,520 - -4.0 950,571 - -1.3 950,686 + -2.7 950,686 + -2.9 950,707 + -1.9 950,707 + -1.1 950,722 + -0.3 950,722 + -1.6 950,772 - pflB b0903 0.12 -3.4 950,772 - pflB b0903 0.12 -1.4 950,777 + pflB b0903 0.12 -2.7 950,836 - pflB b0903 0.15 -3.1 950,836 - pflB b0903 0.15 -0.3 950,854 + pflB b0903 0.16 -3.8 950,944 - pflB b0903 0.20 -3.8 950,974 - pflB b0903 0.21 -2.0 950,974 - pflB b0903 0.21 -3.4 950,975 + pflB b0903 0.21 -4.2 951,040 + pflB b0903 0.24 -1.3 951,040 + pflB b0903 0.24 -1.3 951,120 - pflB b0903 0.27 -3.5 951,193 - pflB b0903 0.31 -1.6 951,269 + pflB b0903 0.34 -2.3 951,269 + pflB b0903 0.34 -2.7 951,281 + pflB b0903 0.34 -2.3 951,288 + pflB b0903 0.35 -0.9
Or see this region's nucleotide sequence