Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybiV and ybiW are separated by 145 nucleotides ybiW and ybiY are separated by 5 nucleotides
b0822: ybiV - predicted hydrolase (NCBI), at 858,436 to 859,251
ybiV
b0823: ybiW - predicted pyruvate formate lyase (NCBI), at 859,397 to 861,829
ybiW
b0824: ybiY - predicted pyruvate formate lyase activating enzyme (RefSeq), at 861,835 to 862,734
ybiY
Position (kb) 859
860
861
862 Strain fitness (log2 ratio) -2
-1
0
1
2 at 858.400 kb on - strand at 858.400 kb on - strand at 858.424 kb on + strand at 858.439 kb on + strand at 858.457 kb on + strand at 858.460 kb on + strand at 858.556 kb on - strand, within ybiV at 858.604 kb on - strand, within ybiV at 858.759 kb on + strand, within ybiV at 858.796 kb on - strand, within ybiV at 858.920 kb on - strand, within ybiV at 859.134 kb on + strand, within ybiV at 859.134 kb on + strand, within ybiV at 859.446 kb on - strand at 859.446 kb on - strand at 859.472 kb on + strand at 859.486 kb on + strand at 859.569 kb on + strand at 859.569 kb on + strand at 859.651 kb on + strand, within ybiW at 859.661 kb on - strand, within ybiW at 859.740 kb on + strand, within ybiW at 859.775 kb on + strand, within ybiW at 859.783 kb on - strand, within ybiW at 859.783 kb on - strand, within ybiW at 859.876 kb on + strand, within ybiW at 859.884 kb on - strand, within ybiW at 859.884 kb on - strand, within ybiW at 859.967 kb on - strand, within ybiW at 860.047 kb on + strand, within ybiW at 860.081 kb on - strand, within ybiW at 860.081 kb on - strand, within ybiW at 860.146 kb on + strand, within ybiW at 860.210 kb on + strand, within ybiW at 860.228 kb on + strand, within ybiW at 860.228 kb on + strand, within ybiW at 860.245 kb on - strand, within ybiW at 860.295 kb on + strand, within ybiW at 860.295 kb on + strand, within ybiW at 860.306 kb on + strand, within ybiW at 860.307 kb on - strand, within ybiW at 860.310 kb on + strand, within ybiW at 860.469 kb on - strand, within ybiW at 860.510 kb on + strand, within ybiW at 860.623 kb on + strand, within ybiW at 860.631 kb on - strand, within ybiW at 860.634 kb on - strand, within ybiW at 860.687 kb on - strand, within ybiW at 860.764 kb on + strand, within ybiW at 860.884 kb on - strand, within ybiW at 861.029 kb on - strand, within ybiW at 861.287 kb on - strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.428 kb on + strand, within ybiW at 861.715 kb on + strand at 861.715 kb on + strand at 861.723 kb on - strand at 861.728 kb on - strand at 861.749 kb on - strand at 861.947 kb on - strand, within ybiY at 862.001 kb on + strand, within ybiY at 862.063 kb on - strand, within ybiY at 862.219 kb on - strand, within ybiY at 862.295 kb on + strand, within ybiY at 862.398 kb on - strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.435 kb on + strand, within ybiY at 862.439 kb on - strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.442 kb on + strand, within ybiY at 862.462 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.575 kb on + strand, within ybiY at 862.580 kb on + strand, within ybiY at 862.600 kb on + strand, within ybiY at 862.697 kb on - strand at 862.717 kb on - strand at 862.828 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 858,400 - -0.3 858,400 - -0.7 858,424 + +0.1 858,439 + -0.2 858,457 + -0.1 858,460 + -0.7 858,556 - ybiV b0822 0.15 -0.5 858,604 - ybiV b0822 0.21 -0.3 858,759 + ybiV b0822 0.40 -1.3 858,796 - ybiV b0822 0.44 -1.0 858,920 - ybiV b0822 0.59 +0.0 859,134 + ybiV b0822 0.86 -2.0 859,134 + ybiV b0822 0.86 +1.0 859,446 - -0.2 859,446 - -1.0 859,472 + +1.0 859,486 + -1.6 859,569 + -0.6 859,569 + -1.2 859,651 + ybiW b0823 0.10 -0.4 859,661 - ybiW b0823 0.11 +1.0 859,740 + ybiW b0823 0.14 +0.3 859,775 + ybiW b0823 0.16 -0.2 859,783 - ybiW b0823 0.16 +0.0 859,783 - ybiW b0823 0.16 -0.9 859,876 + ybiW b0823 0.20 +0.3 859,884 - ybiW b0823 0.20 +0.4 859,884 - ybiW b0823 0.20 +2.1 859,967 - ybiW b0823 0.23 +1.2 860,047 + ybiW b0823 0.27 +0.9 860,081 - ybiW b0823 0.28 -0.0 860,081 - ybiW b0823 0.28 -1.0 860,146 + ybiW b0823 0.31 -0.1 860,210 + ybiW b0823 0.33 -0.6 860,228 + ybiW b0823 0.34 +0.3 860,228 + ybiW b0823 0.34 +1.3 860,245 - ybiW b0823 0.35 +0.2 860,295 + ybiW b0823 0.37 +0.0 860,295 + ybiW b0823 0.37 +0.6 860,306 + ybiW b0823 0.37 +0.5 860,307 - ybiW b0823 0.37 -2.2 860,310 + ybiW b0823 0.38 +0.9 860,469 - ybiW b0823 0.44 +0.4 860,510 + ybiW b0823 0.46 -0.3 860,623 + ybiW b0823 0.50 +1.2 860,631 - ybiW b0823 0.51 +0.2 860,634 - ybiW b0823 0.51 +0.8 860,687 - ybiW b0823 0.53 +0.5 860,764 + ybiW b0823 0.56 +0.5 860,884 - ybiW b0823 0.61 -0.5 861,029 - ybiW b0823 0.67 -0.2 861,287 - ybiW b0823 0.78 -1.2 861,428 + ybiW b0823 0.83 -1.0 861,428 + ybiW b0823 0.83 -1.0 861,428 + ybiW b0823 0.83 +1.1 861,715 + -0.4 861,715 + +0.0 861,723 - +0.9 861,728 - +1.3 861,749 - -0.5 861,947 - ybiY b0824 0.12 -0.5 862,001 + ybiY b0824 0.18 -0.5 862,063 - ybiY b0824 0.25 +1.6 862,219 - ybiY b0824 0.43 +0.4 862,295 + ybiY b0824 0.51 -0.2 862,398 - ybiY b0824 0.63 +0.8 862,435 + ybiY b0824 0.67 -0.9 862,435 + ybiY b0824 0.67 +1.5 862,439 - ybiY b0824 0.67 -0.8 862,442 + ybiY b0824 0.67 -0.8 862,442 + ybiY b0824 0.67 +0.5 862,462 + ybiY b0824 0.70 -0.8 862,575 + ybiY b0824 0.82 +1.0 862,575 + ybiY b0824 0.82 -0.4 862,580 + ybiY b0824 0.83 +0.5 862,600 + ybiY b0824 0.85 +0.5 862,697 - -0.7 862,717 - -0.3 862,828 + +0.8
Or see this region's nucleotide sequence