Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt moaB and moaC are separated by 2 nucleotides moaC and moaD overlap by 8 nucleotides moaD and moaE are separated by 1 nucleotides moaE and ybhL are separated by 136 nucleotides
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516
moaD
b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970
moaE
b0786: ybhL - predicted inner membrane protein (NCBI), at 819,107 to 819,811
ybhL
Position (kb) 818
819 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand at 818.369 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.455 kb on + strand, within moaD at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.566 kb on - strand, within moaE at 818.677 kb on - strand, within moaE at 818.687 kb on - strand, within moaE at 818.764 kb on - strand, within moaE at 818.892 kb on - strand, within moaE at 818.926 kb on + strand at 818.940 kb on - strand at 819.022 kb on + strand at 819.025 kb on - strand at 819.154 kb on + strand at 819.210 kb on + strand, within ybhL at 819.259 kb on + strand, within ybhL at 819.314 kb on + strand, within ybhL at 819.314 kb on + strand, within ybhL at 819.354 kb on + strand, within ybhL at 819.408 kb on + strand, within ybhL at 819.490 kb on + strand, within ybhL at 819.490 kb on + strand, within ybhL at 819.493 kb on + strand, within ybhL at 819.500 kb on + strand, within ybhL at 819.500 kb on + strand, within ybhL at 819.553 kb on - strand, within ybhL at 819.568 kb on - strand, within ybhL at 819.568 kb on - strand, within ybhL at 819.599 kb on + strand, within ybhL at 819.606 kb on + strand, within ybhL at 819.612 kb on - strand, within ybhL at 819.614 kb on + strand, within ybhL at 819.614 kb on + strand, within ybhL at 819.614 kb on + strand, within ybhL at 819.616 kb on + strand, within ybhL at 819.617 kb on + strand, within ybhL at 819.625 kb on - strand, within ybhL at 819.647 kb on - strand, within ybhL at 819.657 kb on + strand, within ybhL at 819.691 kb on + strand, within ybhL at 819.691 kb on + strand, within ybhL at 819.691 kb on + strand, within ybhL at 819.762 kb on + strand at 819.770 kb on - strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.825 kb on + strand at 819.830 kb on + strand at 819.830 kb on + strand at 819.833 kb on - strand at 819.833 kb on - strand at 819.887 kb on + strand at 819.888 kb on + strand at 819.888 kb on + strand at 819.961 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 817,818 - -4.8 817,818 - -2.4 817,852 - moaC b0783 0.12 -2.2 817,852 - moaC b0783 0.12 -1.0 817,872 - moaC b0783 0.16 -2.4 817,912 + moaC b0783 0.24 +0.2 817,912 + moaC b0783 0.24 -1.2 818,006 - moaC b0783 0.44 -1.9 818,012 - moaC b0783 0.45 -0.8 818,167 - moaC b0783 0.77 -1.9 818,211 + moaC b0783 0.86 -2.2 818,219 - moaC b0783 0.88 -1.9 818,219 - moaC b0783 0.88 -2.7 818,252 + -0.4 818,252 + -0.5 818,369 + moaD b0784 0.40 -2.4 818,455 + moaD b0784 0.75 -0.2 818,455 + moaD b0784 0.75 -0.8 818,566 - moaE b0785 0.11 -2.5 818,566 - moaE b0785 0.11 -4.3 818,566 - moaE b0785 0.11 -1.6 818,677 - moaE b0785 0.35 -2.6 818,687 - moaE b0785 0.37 -0.5 818,764 - moaE b0785 0.54 -1.5 818,892 - moaE b0785 0.83 -3.2 818,926 + -0.1 818,940 - -3.3 819,022 + -0.5 819,025 - +0.2 819,154 + +0.3 819,210 + ybhL b0786 0.15 +0.8 819,259 + ybhL b0786 0.22 -1.0 819,314 + ybhL b0786 0.29 +0.9 819,314 + ybhL b0786 0.29 -0.0 819,354 + ybhL b0786 0.35 -1.4 819,408 + ybhL b0786 0.43 +0.2 819,490 + ybhL b0786 0.54 +0.2 819,490 + ybhL b0786 0.54 -3.1 819,493 + ybhL b0786 0.55 -0.2 819,500 + ybhL b0786 0.56 -1.0 819,500 + ybhL b0786 0.56 -0.7 819,553 - ybhL b0786 0.63 +0.5 819,568 - ybhL b0786 0.65 -1.2 819,568 - ybhL b0786 0.65 +0.9 819,599 + ybhL b0786 0.70 -0.5 819,606 + ybhL b0786 0.71 +0.2 819,612 - ybhL b0786 0.72 -0.4 819,614 + ybhL b0786 0.72 +0.4 819,614 + ybhL b0786 0.72 +0.6 819,614 + ybhL b0786 0.72 +0.8 819,616 + ybhL b0786 0.72 +1.0 819,617 + ybhL b0786 0.72 -0.2 819,625 - ybhL b0786 0.73 -0.7 819,647 - ybhL b0786 0.77 -0.2 819,657 + ybhL b0786 0.78 -0.2 819,691 + ybhL b0786 0.83 -1.0 819,691 + ybhL b0786 0.83 +0.2 819,691 + ybhL b0786 0.83 -0.1 819,762 + -1.1 819,770 - +0.8 819,825 + -1.0 819,825 + +0.8 819,825 + -2.0 819,825 + +0.3 819,825 + -0.5 819,825 + -0.3 819,830 + -0.1 819,830 + +0.3 819,833 - +0.8 819,833 - +0.8 819,887 + -0.4 819,888 + +0.8 819,888 + +0.6 819,961 + -0.2
Or see this region's nucleotide sequence