Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ybhK and moaA are separated by 396 nucleotides moaA and moaB are separated by 21 nucleotides moaB and moaC are separated by 2 nucleotides
b0780: ybhK - predicted transferase with NAD(P)-binding Rossmann-fold domain (NCBI), at 814,962 to 815,870
ybhK
b0781: moaA - molybdenum cofactor biosynthesis protein A (NCBI), at 816,267 to 817,256
moaA
b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790
moaB
b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278
moaC
Position (kb) 816
817
818 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1
2 at 815.311 kb on + strand, within ybhK at 815.311 kb on + strand, within ybhK at 815.311 kb on - strand, within ybhK at 815.311 kb on - strand, within ybhK at 815.358 kb on - strand, within ybhK at 815.413 kb on - strand, within ybhK at 815.552 kb on + strand, within ybhK at 815.554 kb on - strand, within ybhK at 815.557 kb on + strand, within ybhK at 815.675 kb on - strand, within ybhK at 815.675 kb on - strand, within ybhK at 815.688 kb on + strand, within ybhK at 815.905 kb on - strand at 816.260 kb on + strand at 816.333 kb on - strand at 816.363 kb on + strand at 816.363 kb on + strand at 816.378 kb on - strand, within moaA at 816.609 kb on - strand, within moaA at 816.624 kb on + strand, within moaA at 816.680 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.746 kb on + strand, within moaA at 816.782 kb on - strand, within moaA at 816.817 kb on + strand, within moaA at 816.912 kb on + strand, within moaA at 817.009 kb on - strand, within moaA at 817.009 kb on - strand, within moaA at 817.049 kb on - strand, within moaA at 817.085 kb on - strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.105 kb on + strand, within moaA at 817.280 kb on - strand at 817.280 kb on - strand at 817.355 kb on - strand, within moaB at 817.485 kb on + strand, within moaB at 817.485 kb on + strand, within moaB at 817.818 kb on - strand at 817.818 kb on - strand at 817.852 kb on - strand, within moaC at 817.852 kb on - strand, within moaC at 817.872 kb on - strand, within moaC at 817.912 kb on + strand, within moaC at 817.912 kb on + strand, within moaC at 818.006 kb on - strand, within moaC at 818.012 kb on - strand, within moaC at 818.167 kb on - strand, within moaC at 818.211 kb on + strand, within moaC at 818.219 kb on - strand, within moaC at 818.219 kb on - strand, within moaC at 818.252 kb on + strand at 818.252 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 815,311 + ybhK b0780 0.38 -0.2 815,311 + ybhK b0780 0.38 +0.6 815,311 - ybhK b0780 0.38 +0.4 815,311 - ybhK b0780 0.38 -0.2 815,358 - ybhK b0780 0.44 -0.2 815,413 - ybhK b0780 0.50 +1.3 815,552 + ybhK b0780 0.65 -0.5 815,554 - ybhK b0780 0.65 -2.2 815,557 + ybhK b0780 0.65 -1.1 815,675 - ybhK b0780 0.78 +0.2 815,675 - ybhK b0780 0.78 +0.5 815,688 + ybhK b0780 0.80 +0.3 815,905 - +0.5 816,260 + -0.4 816,333 - -1.8 816,363 + -0.5 816,363 + +0.1 816,378 - moaA b0781 0.11 -3.0 816,609 - moaA b0781 0.35 +2.4 816,624 + moaA b0781 0.36 -1.9 816,680 + moaA b0781 0.42 -1.1 816,746 + moaA b0781 0.48 -2.8 816,746 + moaA b0781 0.48 -3.4 816,782 - moaA b0781 0.52 -2.2 816,817 + moaA b0781 0.56 -1.2 816,912 + moaA b0781 0.65 -2.5 817,009 - moaA b0781 0.75 -2.5 817,009 - moaA b0781 0.75 +0.3 817,049 - moaA b0781 0.79 -1.5 817,085 - moaA b0781 0.83 -2.8 817,105 + moaA b0781 0.85 -0.8 817,105 + moaA b0781 0.85 -1.7 817,105 + moaA b0781 0.85 -0.9 817,280 - -0.7 817,280 - +1.6 817,355 - moaB b0782 0.15 -2.1 817,485 + moaB b0782 0.40 +1.7 817,485 + moaB b0782 0.40 +0.2 817,818 - -4.8 817,818 - -2.4 817,852 - moaC b0783 0.12 -2.2 817,852 - moaC b0783 0.12 -1.0 817,872 - moaC b0783 0.16 -2.4 817,912 + moaC b0783 0.24 +0.2 817,912 + moaC b0783 0.24 -1.2 818,006 - moaC b0783 0.44 -1.9 818,012 - moaC b0783 0.45 -0.8 818,167 - moaC b0783 0.77 -1.9 818,211 + moaC b0783 0.86 -2.2 818,219 - moaC b0783 0.88 -1.9 818,219 - moaC b0783 0.88 -2.7 818,252 + -0.4 818,252 + -0.5
Or see this region's nucleotide sequence