Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt bioD and uvrB are separated by 578 nucleotides uvrB and ybhK are separated by 191 nucleotides
b0778: bioD - dethiobiotin synthetase (NCBI), at 811,493 to 812,170
bioD
b0779: uvrB - excinuclease ABC subunit B (NCBI), at 812,749 to 814,770
uvrB
b0780: ybhK - predicted transferase with NAD(P)-binding Rossmann-fold domain (NCBI), at 814,962 to 815,870
ybhK
Position (kb) 812
813
814
815 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 811.757 kb on + strand, within bioD at 812.000 kb on + strand, within bioD at 812.005 kb on - strand, within bioD at 812.005 kb on - strand, within bioD at 812.051 kb on + strand, within bioD at 812.051 kb on + strand, within bioD at 812.061 kb on - strand, within bioD at 812.295 kb on - strand at 812.353 kb on - strand at 812.834 kb on + strand at 812.957 kb on + strand, within uvrB at 812.967 kb on - strand, within uvrB at 813.039 kb on + strand, within uvrB at 813.050 kb on + strand, within uvrB at 813.055 kb on - strand, within uvrB at 813.106 kb on - strand, within uvrB at 813.106 kb on - strand, within uvrB at 813.221 kb on - strand, within uvrB at 813.221 kb on - strand, within uvrB at 813.253 kb on - strand, within uvrB at 813.342 kb on + strand, within uvrB at 813.346 kb on + strand, within uvrB at 813.494 kb on + strand, within uvrB at 813.502 kb on - strand, within uvrB at 813.630 kb on + strand, within uvrB at 813.656 kb on + strand, within uvrB at 813.656 kb on + strand, within uvrB at 813.702 kb on + strand, within uvrB at 813.702 kb on + strand, within uvrB at 813.748 kb on - strand, within uvrB at 813.748 kb on - strand, within uvrB at 813.798 kb on + strand, within uvrB at 813.798 kb on + strand, within uvrB at 813.849 kb on + strand, within uvrB at 813.970 kb on + strand, within uvrB at 813.970 kb on + strand, within uvrB at 814.041 kb on + strand, within uvrB at 814.100 kb on + strand, within uvrB at 814.158 kb on - strand, within uvrB at 814.187 kb on - strand, within uvrB at 814.207 kb on + strand, within uvrB at 814.210 kb on - strand, within uvrB at 814.210 kb on - strand, within uvrB at 814.281 kb on - strand, within uvrB at 814.283 kb on + strand, within uvrB at 814.492 kb on + strand, within uvrB at 814.675 kb on + strand at 814.818 kb on - strand at 814.818 kb on - strand at 814.842 kb on - strand at 814.972 kb on - strand at 815.024 kb on + strand at 815.024 kb on + strand at 815.048 kb on - strand at 815.311 kb on + strand, within ybhK at 815.311 kb on + strand, within ybhK at 815.311 kb on - strand, within ybhK at 815.311 kb on - strand, within ybhK at 815.358 kb on - strand, within ybhK at 815.413 kb on - strand, within ybhK at 815.552 kb on + strand, within ybhK at 815.554 kb on - strand, within ybhK at 815.557 kb on + strand, within ybhK at 815.675 kb on - strand, within ybhK at 815.675 kb on - strand, within ybhK at 815.688 kb on + strand, within ybhK
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 811,757 + bioD b0778 0.39 -0.1 812,000 + bioD b0778 0.75 +0.2 812,005 - bioD b0778 0.76 -2.2 812,005 - bioD b0778 0.76 +1.2 812,051 + bioD b0778 0.82 +2.4 812,051 + bioD b0778 0.82 +0.6 812,061 - bioD b0778 0.84 -0.8 812,295 - +0.5 812,353 - -0.7 812,834 + -3.5 812,957 + uvrB b0779 0.10 -1.8 812,967 - uvrB b0779 0.11 -2.0 813,039 + uvrB b0779 0.14 -3.8 813,050 + uvrB b0779 0.15 -2.4 813,055 - uvrB b0779 0.15 -3.8 813,106 - uvrB b0779 0.18 -3.8 813,106 - uvrB b0779 0.18 -0.2 813,221 - uvrB b0779 0.23 -0.8 813,221 - uvrB b0779 0.23 -0.2 813,253 - uvrB b0779 0.25 -4.1 813,342 + uvrB b0779 0.29 -2.4 813,346 + uvrB b0779 0.30 -3.1 813,494 + uvrB b0779 0.37 -2.4 813,502 - uvrB b0779 0.37 -2.4 813,630 + uvrB b0779 0.44 -3.0 813,656 + uvrB b0779 0.45 -3.1 813,656 + uvrB b0779 0.45 -2.0 813,702 + uvrB b0779 0.47 -3.7 813,702 + uvrB b0779 0.47 -1.5 813,748 - uvrB b0779 0.49 -2.4 813,748 - uvrB b0779 0.49 +0.8 813,798 + uvrB b0779 0.52 -2.7 813,798 + uvrB b0779 0.52 -3.0 813,849 + uvrB b0779 0.54 -0.8 813,970 + uvrB b0779 0.60 -3.9 813,970 + uvrB b0779 0.60 -4.4 814,041 + uvrB b0779 0.64 -4.0 814,100 + uvrB b0779 0.67 -2.8 814,158 - uvrB b0779 0.70 -2.2 814,187 - uvrB b0779 0.71 -0.9 814,207 + uvrB b0779 0.72 -3.0 814,210 - uvrB b0779 0.72 -2.5 814,210 - uvrB b0779 0.72 -2.7 814,281 - uvrB b0779 0.76 -1.2 814,283 + uvrB b0779 0.76 -2.0 814,492 + uvrB b0779 0.86 -3.7 814,675 + -1.4 814,818 - -0.9 814,818 - -0.7 814,842 - -1.7 814,972 - -0.6 815,024 + +0.8 815,024 + +1.0 815,048 - +0.7 815,311 + ybhK b0780 0.38 -0.2 815,311 + ybhK b0780 0.38 +0.6 815,311 - ybhK b0780 0.38 +0.4 815,311 - ybhK b0780 0.38 -0.2 815,358 - ybhK b0780 0.44 -0.2 815,413 - ybhK b0780 0.50 +1.3 815,552 + ybhK b0780 0.65 -0.5 815,554 - ybhK b0780 0.65 -2.2 815,557 + ybhK b0780 0.65 -1.1 815,675 - ybhK b0780 0.78 +0.2 815,675 - ybhK b0780 0.78 +0.5 815,688 + ybhK b0780 0.80 +0.3
Or see this region's nucleotide sequence