Experiment: LB Anaerobic with Nitrofurantoin 0.000015625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cusA and pheP are separated by 101 nucleotides pheP and ybdG are separated by 80 nucleotides
b0575: cusA - copper/silver efflux system, membrane component (NCBI), at 597,937 to 601,080
cusA
b0576: pheP - phenylalanine transporter (NCBI), at 601,182 to 602,558
pheP
b0577: ybdG - predicted mechanosensitive channel (NCBI), at 602,639 to 603,886
ybdG
Position (kb) 601
602
603 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 600.220 kb on - strand, within cusA at 600.288 kb on - strand, within cusA at 600.426 kb on + strand, within cusA at 600.426 kb on + strand, within cusA at 600.517 kb on - strand, within cusA at 600.517 kb on - strand, within cusA at 600.743 kb on - strand, within cusA at 600.769 kb on - strand at 600.769 kb on - strand at 600.793 kb on - strand at 600.804 kb on - strand at 600.868 kb on + strand at 600.904 kb on - strand at 600.958 kb on - strand at 600.958 kb on - strand at 601.069 kb on - strand at 601.205 kb on + strand at 601.248 kb on + strand at 601.258 kb on + strand at 601.279 kb on - strand at 601.288 kb on - strand at 601.293 kb on - strand at 601.378 kb on + strand, within pheP at 601.441 kb on - strand, within pheP at 601.462 kb on + strand, within pheP at 601.552 kb on + strand, within pheP at 601.616 kb on + strand, within pheP at 601.624 kb on + strand, within pheP at 601.852 kb on + strand, within pheP at 601.852 kb on + strand, within pheP at 601.876 kb on + strand, within pheP at 601.876 kb on + strand at 601.957 kb on + strand, within pheP at 601.987 kb on - strand, within pheP at 601.988 kb on - strand, within pheP at 602.108 kb on - strand, within pheP at 602.156 kb on + strand, within pheP at 602.181 kb on + strand, within pheP at 602.181 kb on + strand, within pheP at 602.194 kb on + strand, within pheP at 602.194 kb on + strand, within pheP at 602.265 kb on - strand, within pheP at 602.393 kb on + strand, within pheP at 602.393 kb on + strand, within pheP at 602.413 kb on - strand, within pheP at 602.465 kb on + strand at 602.465 kb on + strand at 602.475 kb on + strand at 602.478 kb on - strand at 602.478 kb on - strand at 602.480 kb on - strand at 602.496 kb on + strand at 602.508 kb on + strand at 602.508 kb on + strand at 602.549 kb on - strand at 602.571 kb on - strand at 602.582 kb on + strand at 602.583 kb on + strand at 602.584 kb on + strand at 602.593 kb on + strand at 602.595 kb on + strand at 602.603 kb on - strand at 602.605 kb on + strand at 602.605 kb on + strand at 602.605 kb on + strand at 602.610 kb on + strand at 602.613 kb on - strand at 602.664 kb on + strand at 602.679 kb on - strand at 602.682 kb on + strand at 602.682 kb on + strand at 602.682 kb on + strand at 602.694 kb on - strand at 602.699 kb on + strand at 602.699 kb on + strand at 602.705 kb on + strand at 602.705 kb on - strand at 602.706 kb on - strand at 602.767 kb on - strand, within ybdG at 602.774 kb on + strand, within ybdG at 602.802 kb on - strand, within ybdG at 602.806 kb on - strand, within ybdG at 602.816 kb on + strand, within ybdG at 602.816 kb on + strand, within ybdG at 602.817 kb on - strand, within ybdG at 602.829 kb on - strand, within ybdG at 602.829 kb on - strand, within ybdG at 602.831 kb on + strand, within ybdG at 602.831 kb on - strand, within ybdG at 602.833 kb on + strand, within ybdG at 602.839 kb on - strand, within ybdG at 602.843 kb on + strand, within ybdG at 602.843 kb on + strand, within ybdG at 602.850 kb on - strand, within ybdG at 602.850 kb on - strand, within ybdG at 602.874 kb on + strand, within ybdG at 602.874 kb on + strand, within ybdG at 602.884 kb on - strand, within ybdG at 602.884 kb on - strand, within ybdG at 602.884 kb on - strand, within ybdG at 602.884 kb on - strand, within ybdG at 602.896 kb on + strand, within ybdG at 602.916 kb on + strand, within ybdG at 602.918 kb on - strand, within ybdG at 602.918 kb on - strand, within ybdG at 602.918 kb on - strand, within ybdG at 602.919 kb on - strand, within ybdG at 602.919 kb on - strand, within ybdG at 602.936 kb on + strand, within ybdG at 602.937 kb on + strand, within ybdG at 602.937 kb on + strand, within ybdG at 602.940 kb on + strand, within ybdG at 602.944 kb on - strand, within ybdG at 602.948 kb on - strand, within ybdG at 602.960 kb on - strand, within ybdG at 602.960 kb on - strand, within ybdG at 602.973 kb on - strand, within ybdG at 603.065 kb on + strand, within ybdG at 603.079 kb on + strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.084 kb on - strand, within ybdG at 603.088 kb on - strand, within ybdG at 603.088 kb on - strand, within ybdG at 603.107 kb on - strand, within ybdG at 603.107 kb on - strand, within ybdG at 603.130 kb on - strand, within ybdG at 603.130 kb on - strand, within ybdG at 603.134 kb on + strand, within ybdG at 603.163 kb on + strand, within ybdG at 603.164 kb on + strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.172 kb on - strand, within ybdG at 603.196 kb on - strand, within ybdG at 603.196 kb on - strand, within ybdG at 603.197 kb on - strand, within ybdG at 603.197 kb on - strand, within ybdG at 603.225 kb on - strand, within ybdG at 603.277 kb on + strand, within ybdG at 603.301 kb on + strand, within ybdG at 603.312 kb on - strand, within ybdG at 603.347 kb on + strand, within ybdG at 603.355 kb on - strand, within ybdG at 603.369 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.375 kb on - strand, within ybdG at 603.380 kb on + strand, within ybdG at 603.402 kb on + strand, within ybdG at 603.402 kb on + strand, within ybdG at 603.403 kb on - strand, within ybdG at 603.403 kb on - strand, within ybdG at 603.408 kb on - strand, within ybdG at 603.417 kb on - strand, within ybdG at 603.436 kb on + strand, within ybdG at 603.444 kb on - strand, within ybdG at 603.446 kb on + strand, within ybdG at 603.457 kb on - strand, within ybdG at 603.479 kb on - strand, within ybdG at 603.528 kb on + strand, within ybdG at 603.538 kb on - strand, within ybdG at 603.540 kb on - strand, within ybdG at 603.540 kb on - strand, within ybdG at 603.542 kb on + strand, within ybdG at 603.551 kb on - strand, within ybdG at 603.553 kb on + strand, within ybdG at 603.553 kb on + strand, within ybdG at 603.553 kb on + strand, within ybdG at 603.558 kb on - strand, within ybdG at 603.558 kb on - strand, within ybdG at 603.558 kb on - strand, within ybdG
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Nitrofurantoin 0.000015625 mM remove 600,220 - cusA b0575 0.73 +0.3 600,288 - cusA b0575 0.75 +1.2 600,426 + cusA b0575 0.79 -0.5 600,426 + cusA b0575 0.79 +0.3 600,517 - cusA b0575 0.82 -0.1 600,517 - cusA b0575 0.82 -1.7 600,743 - cusA b0575 0.89 +0.3 600,769 - -0.4 600,769 - +0.1 600,793 - +0.5 600,804 - -2.5 600,868 + -0.7 600,904 - +1.3 600,958 - -2.1 600,958 - +0.3 601,069 - +0.3 601,205 + -0.5 601,248 + -0.4 601,258 + -0.4 601,279 - +0.2 601,288 - -0.7 601,293 - -0.6 601,378 + pheP b0576 0.14 +0.8 601,441 - pheP b0576 0.19 +0.9 601,462 + pheP b0576 0.20 -0.1 601,552 + pheP b0576 0.27 -2.1 601,616 + pheP b0576 0.32 -0.4 601,624 + pheP b0576 0.32 +0.1 601,852 + pheP b0576 0.49 +0.7 601,852 + pheP b0576 0.49 +0.1 601,876 + pheP b0576 0.50 -2.4 601,876 + +1.5 601,957 + pheP b0576 0.56 +0.4 601,987 - pheP b0576 0.58 -1.1 601,988 - pheP b0576 0.59 -0.8 602,108 - pheP b0576 0.67 +0.0 602,156 + pheP b0576 0.71 +0.6 602,181 + pheP b0576 0.73 +0.6 602,181 + pheP b0576 0.73 +0.1 602,194 + pheP b0576 0.73 -0.3 602,194 + pheP b0576 0.73 -0.7 602,265 - pheP b0576 0.79 +2.5 602,393 + pheP b0576 0.88 -0.8 602,393 + pheP b0576 0.88 +1.3 602,413 - pheP b0576 0.89 +0.5 602,465 + +0.2 602,465 + +0.7 602,475 + +0.3 602,478 - -1.1 602,478 - -0.1 602,480 - +0.9 602,496 + -0.4 602,508 + -0.7 602,508 + -1.1 602,549 - -1.7 602,571 - +2.5 602,582 + +1.2 602,583 + -1.1 602,584 + -0.7 602,593 + -0.4 602,595 + -0.7 602,603 - +0.9 602,605 + -0.9 602,605 + +1.8 602,605 + -0.2 602,610 + -0.2 602,613 - -1.4 602,664 + -0.7 602,679 - -0.2 602,682 + -0.2 602,682 + +0.2 602,682 + +1.2 602,694 - +0.9 602,699 + -1.4 602,699 + +0.3 602,705 + +1.7 602,705 - -0.1 602,706 - +0.4 602,767 - ybdG b0577 0.10 +0.0 602,774 + ybdG b0577 0.11 -1.2 602,802 - ybdG b0577 0.13 +0.6 602,806 - ybdG b0577 0.13 +0.4 602,816 + ybdG b0577 0.14 -0.3 602,816 + ybdG b0577 0.14 -0.1 602,817 - ybdG b0577 0.14 -0.8 602,829 - ybdG b0577 0.15 -0.1 602,829 - ybdG b0577 0.15 +0.3 602,831 + ybdG b0577 0.15 +0.6 602,831 - ybdG b0577 0.15 -0.0 602,833 + ybdG b0577 0.16 +0.3 602,839 - ybdG b0577 0.16 +0.5 602,843 + ybdG b0577 0.16 -0.1 602,843 + ybdG b0577 0.16 -0.9 602,850 - ybdG b0577 0.17 -1.9 602,850 - ybdG b0577 0.17 -0.2 602,874 + ybdG b0577 0.19 -0.8 602,874 + ybdG b0577 0.19 -0.9 602,884 - ybdG b0577 0.20 -0.3 602,884 - ybdG b0577 0.20 +0.3 602,884 - ybdG b0577 0.20 -0.8 602,884 - ybdG b0577 0.20 -0.4 602,896 + ybdG b0577 0.21 -0.4 602,916 + ybdG b0577 0.22 +0.3 602,918 - ybdG b0577 0.22 +0.7 602,918 - ybdG b0577 0.22 +2.5 602,918 - ybdG b0577 0.22 +0.4 602,919 - ybdG b0577 0.22 +0.7 602,919 - ybdG b0577 0.22 -1.4 602,936 + ybdG b0577 0.24 +3.2 602,937 + ybdG b0577 0.24 +0.1 602,937 + ybdG b0577 0.24 -1.7 602,940 + ybdG b0577 0.24 +0.3 602,944 - ybdG b0577 0.24 +0.4 602,948 - ybdG b0577 0.25 +0.0 602,960 - ybdG b0577 0.26 +0.7 602,960 - ybdG b0577 0.26 +0.9 602,973 - ybdG b0577 0.27 +1.9 603,065 + ybdG b0577 0.34 +0.9 603,079 + ybdG b0577 0.35 +0.9 603,084 - ybdG b0577 0.36 -0.3 603,084 - ybdG b0577 0.36 +0.7 603,084 - ybdG b0577 0.36 -0.4 603,084 - ybdG b0577 0.36 +0.6 603,084 - ybdG b0577 0.36 +0.5 603,084 - ybdG b0577 0.36 +0.9 603,084 - ybdG b0577 0.36 -0.9 603,088 - ybdG b0577 0.36 +1.0 603,088 - ybdG b0577 0.36 +0.3 603,107 - ybdG b0577 0.38 +0.7 603,107 - ybdG b0577 0.38 -1.0 603,130 - ybdG b0577 0.39 -2.1 603,130 - ybdG b0577 0.39 +0.4 603,134 + ybdG b0577 0.40 +0.3 603,163 + ybdG b0577 0.42 -0.1 603,164 + ybdG b0577 0.42 -1.4 603,172 - ybdG b0577 0.43 +0.8 603,172 - ybdG b0577 0.43 -0.4 603,172 - ybdG b0577 0.43 +0.1 603,172 - ybdG b0577 0.43 -0.1 603,172 - ybdG b0577 0.43 +0.5 603,172 - ybdG b0577 0.43 -0.8 603,196 - ybdG b0577 0.45 -1.5 603,196 - ybdG b0577 0.45 +0.5 603,197 - ybdG b0577 0.45 +0.6 603,197 - ybdG b0577 0.45 +0.6 603,225 - ybdG b0577 0.47 +1.2 603,277 + ybdG b0577 0.51 -1.1 603,301 + ybdG b0577 0.53 -0.1 603,312 - ybdG b0577 0.54 -0.2 603,347 + ybdG b0577 0.57 -0.2 603,355 - ybdG b0577 0.57 -0.4 603,369 - ybdG b0577 0.58 +0.7 603,375 - ybdG b0577 0.59 -0.6 603,375 - ybdG b0577 0.59 +0.5 603,375 - ybdG b0577 0.59 -0.4 603,375 - ybdG b0577 0.59 -1.4 603,375 - ybdG b0577 0.59 -0.0 603,380 + ybdG b0577 0.59 +0.9 603,402 + ybdG b0577 0.61 -0.3 603,402 + ybdG b0577 0.61 +0.2 603,403 - ybdG b0577 0.61 -0.8 603,403 - ybdG b0577 0.61 +0.4 603,408 - ybdG b0577 0.62 -0.3 603,417 - ybdG b0577 0.62 +0.5 603,436 + ybdG b0577 0.64 +0.6 603,444 - ybdG b0577 0.65 -2.8 603,446 + ybdG b0577 0.65 +0.1 603,457 - ybdG b0577 0.66 -2.4 603,479 - ybdG b0577 0.67 -0.7 603,528 + ybdG b0577 0.71 +2.3 603,538 - ybdG b0577 0.72 -0.5 603,540 - ybdG b0577 0.72 +1.2 603,540 - ybdG b0577 0.72 -1.1 603,542 + ybdG b0577 0.72 +0.3 603,551 - ybdG b0577 0.73 -0.1 603,553 + ybdG b0577 0.73 +0.9 603,553 + ybdG b0577 0.73 +0.9 603,553 + ybdG b0577 0.73 +0.3 603,558 - ybdG b0577 0.74 +1.6 603,558 - ybdG b0577 0.74 +0.9 603,558 - ybdG b0577 0.74 +0.2
Or see this region's nucleotide sequence