Strain Fitness in Escherichia coli BW25113 around b1817

Experiment: LB, Anaerobic

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyoaE and manX are separated by 462 nucleotidesmanX and manY are separated by 62 nucleotidesmanY and manZ are separated by 12 nucleotides b1816: yoaE - fused predicted membrane protein/conserved protein (NCBI), at 1,898,053 to 1,899,609 yoaE b1817: manX - fused mannose-specific PTS enzymes: IIA component/IIB component (NCBI), at 1,900,072 to 1,901,043 manX b1818: manY - mannose-specific enzyme IIC component of PTS (NCBI), at 1,901,106 to 1,901,906 manY b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770 manZ Position (kb) 1900 1901 1902Strain fitness (log2 ratio) -1 0 1 2at 1899.103 kb on + strand, within yoaEat 1899.103 kb on + strand, within yoaEat 1899.429 kb on + strand, within yoaEat 1899.689 kb on + strandat 1899.769 kb on + strandat 1899.804 kb on + strandat 1899.991 kb on - strandat 1900.072 kb on - strandat 1900.112 kb on - strandat 1900.117 kb on - strandat 1900.225 kb on + strand, within manXat 1900.253 kb on - strand, within manXat 1900.413 kb on + strand, within manXat 1900.611 kb on + strand, within manXat 1900.651 kb on - strand, within manXat 1900.702 kb on + strand, within manXat 1900.730 kb on + strand, within manXat 1900.787 kb on + strand, within manXat 1900.864 kb on - strand, within manXat 1900.885 kb on - strand, within manXat 1900.885 kb on - strand, within manXat 1900.887 kb on + strand, within manXat 1900.887 kb on + strand, within manXat 1900.964 kb on + strandat 1900.972 kb on - strandat 1901.109 kb on - strandat 1901.109 kb on - strandat 1901.147 kb on + strandat 1901.150 kb on - strandat 1901.150 kb on - strandat 1901.406 kb on + strand, within manYat 1901.560 kb on - strand, within manYat 1901.794 kb on - strand, within manY

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Per-strain Table

Position Strand Gene LocusTag Fraction LB, Anaerobic
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1,899,103 + yoaE b1816 0.67 +0.7
1,899,103 + yoaE b1816 0.67 -1.6
1,899,429 + yoaE b1816 0.88 +0.4
1,899,689 + +0.3
1,899,769 + +0.8
1,899,804 + +0.4
1,899,991 - -0.6
1,900,072 - +0.4
1,900,112 - -1.6
1,900,117 - +0.0
1,900,225 + manX b1817 0.16 +0.0
1,900,253 - manX b1817 0.19 -1.7
1,900,413 + manX b1817 0.35 +0.7
1,900,611 + manX b1817 0.55 -0.2
1,900,651 - manX b1817 0.60 +0.0
1,900,702 + manX b1817 0.65 -0.3
1,900,730 + manX b1817 0.68 -0.2
1,900,787 + manX b1817 0.74 +0.2
1,900,864 - manX b1817 0.81 -0.5
1,900,885 - manX b1817 0.84 +0.7
1,900,885 - manX b1817 0.84 -0.7
1,900,887 + manX b1817 0.84 +1.0
1,900,887 + manX b1817 0.84 +0.4
1,900,964 + +2.4
1,900,972 - -1.2
1,901,109 - +2.4
1,901,109 - -0.3
1,901,147 + -0.6
1,901,150 - +0.2
1,901,150 - +0.0
1,901,406 + manY b1818 0.37 -0.4
1,901,560 - manY b1818 0.57 -0.3
1,901,794 - manY b1818 0.86 +0.1

Or see this region's nucleotide sequence