Strain Fitness in Escherichia coli BW25113 around b0783

Experiment: LB Anaerobic with Hygromycin B 0.00025 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmoaA and moaB are separated by 21 nucleotidesmoaB and moaC are separated by 2 nucleotidesmoaC and moaD overlap by 8 nucleotidesmoaD and moaE are separated by 1 nucleotidesmoaE and ybhL are separated by 136 nucleotides b0781: moaA - molybdenum cofactor biosynthesis protein A (NCBI), at 816,267 to 817,256 moaA b0782: moaB - molybdopterin biosynthesis protein B (NCBI), at 817,278 to 817,790 moaB b0783: moaC - molybdenum cofactor biosynthesis protein C (NCBI), at 817,793 to 818,278 moaC b0784: moaD - molybdopterin synthase, small subunit (NCBI), at 818,271 to 818,516 moaD b0785: moaE - molybdopterin synthase, large subunit (NCBI), at 818,518 to 818,970 moaE b0786: ybhL - predicted inner membrane protein (NCBI), at 819,107 to 819,811 ybhL Position (kb) 817 818 819Strain fitness (log2 ratio) -4 -3 -2 -1 0 1at 816.817 kb on + strand, within moaAat 816.912 kb on + strand, within moaAat 817.009 kb on - strand, within moaAat 817.009 kb on - strand, within moaAat 817.049 kb on - strand, within moaAat 817.085 kb on - strand, within moaAat 817.105 kb on + strand, within moaAat 817.105 kb on + strand, within moaAat 817.105 kb on + strand, within moaAat 817.280 kb on - strandat 817.280 kb on - strandat 817.355 kb on - strand, within moaBat 817.485 kb on + strand, within moaBat 817.485 kb on + strand, within moaBat 817.818 kb on - strandat 817.818 kb on - strandat 817.852 kb on - strand, within moaCat 817.852 kb on - strand, within moaCat 817.872 kb on - strand, within moaCat 817.912 kb on + strand, within moaCat 817.912 kb on + strand, within moaCat 818.006 kb on - strand, within moaCat 818.012 kb on - strand, within moaCat 818.167 kb on - strand, within moaCat 818.211 kb on + strand, within moaCat 818.219 kb on - strand, within moaCat 818.219 kb on - strand, within moaCat 818.252 kb on + strandat 818.252 kb on + strandat 818.369 kb on + strand, within moaDat 818.455 kb on + strand, within moaDat 818.455 kb on + strand, within moaDat 818.566 kb on - strand, within moaEat 818.566 kb on - strand, within moaEat 818.566 kb on - strand, within moaEat 818.677 kb on - strand, within moaEat 818.687 kb on - strand, within moaEat 818.764 kb on - strand, within moaEat 818.892 kb on - strand, within moaEat 818.926 kb on + strandat 818.940 kb on - strandat 819.022 kb on + strandat 819.025 kb on - strandat 819.154 kb on + strandat 819.210 kb on + strand, within ybhLat 819.259 kb on + strand, within ybhL

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Hygromycin B 0.00025 mM
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816,817 + moaA b0781 0.56 -1.2
816,912 + moaA b0781 0.65 -1.9
817,009 - moaA b0781 0.75 -0.9
817,009 - moaA b0781 0.75 -2.0
817,049 - moaA b0781 0.79 -1.1
817,085 - moaA b0781 0.83 -3.9
817,105 + moaA b0781 0.85 -0.8
817,105 + moaA b0781 0.85 -2.2
817,105 + moaA b0781 0.85 +0.1
817,280 - -0.1
817,280 - -1.2
817,355 - moaB b0782 0.15 -0.8
817,485 + moaB b0782 0.40 +1.1
817,485 + moaB b0782 0.40 -0.1
817,818 - -1.6
817,818 - +0.6
817,852 - moaC b0783 0.12 -2.2
817,852 - moaC b0783 0.12 -1.0
817,872 - moaC b0783 0.16 -1.4
817,912 + moaC b0783 0.24 +0.2
817,912 + moaC b0783 0.24 -0.6
818,006 - moaC b0783 0.44 -1.3
818,012 - moaC b0783 0.45 -0.8
818,167 - moaC b0783 0.77 -1.2
818,211 + moaC b0783 0.86 -2.6
818,219 - moaC b0783 0.88 -1.2
818,219 - moaC b0783 0.88 -1.7
818,252 + -0.4
818,252 + -1.5
818,369 + moaD b0784 0.40 -1.3
818,455 + moaD b0784 0.75 -0.2
818,455 + moaD b0784 0.75 -0.2
818,566 - moaE b0785 0.11 -0.5
818,566 - moaE b0785 0.11 -2.3
818,566 - moaE b0785 0.11 -1.2
818,677 - moaE b0785 0.35 -1.8
818,687 - moaE b0785 0.37 -2.5
818,764 - moaE b0785 0.54 -1.2
818,892 - moaE b0785 0.83 -1.6
818,926 + -2.3
818,940 - -2.3
819,022 + -0.2
819,025 - -1.3
819,154 + -1.0
819,210 + ybhL b0786 0.15 +1.0
819,259 + ybhL b0786 0.22 +0.1

Or see this region's nucleotide sequence