Strain Fitness in Escherichia coli BW25113 around b2491

Experiment: LB Anaerobic with Tetracycline 0.000000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthyfI and b2490 overlap by 8 nucleotidesb2490 and hyfR are separated by 29 nucleotideshyfR and focB are separated by 21 nucleotidesfocB and yfgO are separated by 37 nucleotides b2489: hyfI - hydrogenase 4, Fe-S subunit (NCBI), at 2,608,728 to 2,609,486 hyfI b2490: b2490 - putative protein processing element (VIMSS), at 2,609,479 to 2,609,892 b2490 b2491: hyfR - putative 2-component regulator, interaction with sigma 54 (VIMSS), at 2,609,922 to 2,611,934 hyfR b2492: focB - predicted formate transporter (NCBI), at 2,611,956 to 2,612,804 focB b2493: yfgO - predicted inner membrane protein (NCBI), at 2,612,842 to 2,613,903 yfgO Position (kb) 2609 2610 2611 2612Strain fitness (log2 ratio) -1 0 1at 2609.013 kb on + strand, within hyfIat 2609.013 kb on + strand, within hyfIat 2609.095 kb on - strand, within hyfIat 2609.097 kb on + strand, within hyfIat 2609.290 kb on - strand, within hyfIat 2609.329 kb on + strand, within hyfIat 2609.329 kb on + strand, within hyfIat 2609.393 kb on - strand, within hyfIat 2609.393 kb on - strand, within hyfIat 2609.480 kb on + strandat 2609.482 kb on + strandat 2609.490 kb on - strandat 2609.490 kb on - strandat 2609.576 kb on - strand, within b2490at 2609.576 kb on - strand, within b2490at 2609.595 kb on - strand, within b2490at 2609.671 kb on - strand, within b2490at 2610.075 kb on - strandat 2610.080 kb on - strandat 2610.149 kb on - strand, within hyfRat 2610.261 kb on + strand, within hyfRat 2610.284 kb on - strand, within hyfRat 2610.286 kb on - strand, within hyfRat 2610.406 kb on + strand, within hyfRat 2610.411 kb on + strand, within hyfRat 2610.555 kb on + strand, within hyfRat 2610.555 kb on + strand, within hyfRat 2610.733 kb on - strand, within hyfRat 2610.768 kb on - strand, within hyfRat 2610.768 kb on - strand, within hyfRat 2610.852 kb on - strand, within hyfRat 2610.852 kb on - strand, within hyfRat 2611.065 kb on + strand, within hyfRat 2611.090 kb on + strand, within hyfRat 2611.139 kb on - strand, within hyfRat 2611.139 kb on - strand, within hyfRat 2611.289 kb on + strand, within hyfRat 2611.392 kb on - strand, within hyfRat 2611.599 kb on - strand, within hyfRat 2611.599 kb on - strand, within hyfRat 2611.829 kb on - strandat 2611.950 kb on + strandat 2612.067 kb on - strand, within focBat 2612.067 kb on - strand, within focBat 2612.133 kb on - strand, within focBat 2612.145 kb on + strand, within focBat 2612.214 kb on + strand, within focBat 2612.214 kb on + strand, within focBat 2612.217 kb on - strand, within focBat 2612.217 kb on - strand, within focBat 2612.397 kb on + strand, within focBat 2612.425 kb on + strand, within focBat 2612.497 kb on - strand, within focBat 2612.579 kb on + strand, within focBat 2612.579 kb on + strand, within focBat 2612.589 kb on - strand, within focBat 2612.690 kb on - strand, within focBat 2612.690 kb on - strand, within focBat 2612.720 kb on - strandat 2612.720 kb on - strandat 2612.749 kb on + strandat 2612.749 kb on + strandat 2612.764 kb on + strandat 2612.764 kb on + strandat 2612.845 kb on - strandat 2612.845 kb on - strandat 2612.875 kb on - strandat 2612.875 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Tetracycline 0.000000078125 mM
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2,609,013 + hyfI b2489 0.38 +0.5
2,609,013 + hyfI b2489 0.38 -0.8
2,609,095 - hyfI b2489 0.48 -0.6
2,609,097 + hyfI b2489 0.49 +0.7
2,609,290 - hyfI b2489 0.74 +0.0
2,609,329 + hyfI b2489 0.79 -0.3
2,609,329 + hyfI b2489 0.79 -0.3
2,609,393 - hyfI b2489 0.88 -0.8
2,609,393 - hyfI b2489 0.88 -0.7
2,609,480 + -0.3
2,609,482 + +0.4
2,609,490 - -0.3
2,609,490 - +0.3
2,609,576 - b2490 b2490 0.23 -0.6
2,609,576 - b2490 b2490 0.23 +0.1
2,609,595 - b2490 b2490 0.28 +0.6
2,609,671 - b2490 b2490 0.46 -0.0
2,610,075 - +0.4
2,610,080 - -0.2
2,610,149 - hyfR b2491 0.11 +0.1
2,610,261 + hyfR b2491 0.17 +1.2
2,610,284 - hyfR b2491 0.18 +0.3
2,610,286 - hyfR b2491 0.18 +0.1
2,610,406 + hyfR b2491 0.24 +0.0
2,610,411 + hyfR b2491 0.24 +0.7
2,610,555 + hyfR b2491 0.31 -1.2
2,610,555 + hyfR b2491 0.31 +0.3
2,610,733 - hyfR b2491 0.40 +0.5
2,610,768 - hyfR b2491 0.42 -0.8
2,610,768 - hyfR b2491 0.42 +0.4
2,610,852 - hyfR b2491 0.46 +0.4
2,610,852 - hyfR b2491 0.46 -0.1
2,611,065 + hyfR b2491 0.57 +0.3
2,611,090 + hyfR b2491 0.58 +0.2
2,611,139 - hyfR b2491 0.60 +0.9
2,611,139 - hyfR b2491 0.60 +0.2
2,611,289 + hyfR b2491 0.68 +0.7
2,611,392 - hyfR b2491 0.73 +0.4
2,611,599 - hyfR b2491 0.83 -0.9
2,611,599 - hyfR b2491 0.83 +0.3
2,611,829 - +0.2
2,611,950 + +0.2
2,612,067 - focB b2492 0.13 +0.3
2,612,067 - focB b2492 0.13 -0.3
2,612,133 - focB b2492 0.21 -0.4
2,612,145 + focB b2492 0.22 +0.3
2,612,214 + focB b2492 0.30 -0.3
2,612,214 + focB b2492 0.30 -1.3
2,612,217 - focB b2492 0.31 -1.3
2,612,217 - focB b2492 0.31 +0.7
2,612,397 + focB b2492 0.52 -0.9
2,612,425 + focB b2492 0.55 +0.7
2,612,497 - focB b2492 0.64 +0.5
2,612,579 + focB b2492 0.73 +0.5
2,612,579 + focB b2492 0.73 -0.2
2,612,589 - focB b2492 0.75 -0.0
2,612,690 - focB b2492 0.86 -0.1
2,612,690 - focB b2492 0.86 -0.1
2,612,720 - -0.2
2,612,720 - +1.7
2,612,749 + -0.5
2,612,749 + -0.3
2,612,764 + +0.2
2,612,764 + +0.3
2,612,845 - +0.1
2,612,845 - -0.4
2,612,875 - -0.6
2,612,875 - +0.4

Or see this region's nucleotide sequence