Experiment: KB with Methyl-2-Hydroxy-Phenylproprionate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1324 and Psyr_1325 are separated by 96 nucleotides Psyr_1325 and Psyr_1326 are separated by 71 nucleotides Psyr_1326 and Psyr_1327 are separated by 204 nucleotides Psyr_1327 and Psyr_1328 are separated by 113 nucleotides
Psyr_1324: Psyr_1324 - conserved hypothetical protein, at 1,501,766 to 1,502,389
_1324
Psyr_1325: Psyr_1325 - Protein of unknown function DUF548, at 1,502,486 to 1,503,295
_1325
Psyr_1326: Psyr_1326 - conserved hypothetical protein, at 1,503,367 to 1,504,056
_1326
Psyr_1327: Psyr_1327 - conserved hypothetical protein, at 1,504,261 to 1,504,611
_1327
Psyr_1328: Psyr_1328 - glycerol-3-phosphate acyltransferase, at 1,504,725 to 1,507,226
_1328
Position (kb)
1503
1504
1505 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1502.389 kb on + strand at 1502.400 kb on - strand at 1502.430 kb on - strand at 1502.479 kb on - strand at 1503.014 kb on + strand, within Psyr_1325 at 1503.014 kb on + strand, within Psyr_1325 at 1503.015 kb on - strand, within Psyr_1325 at 1503.015 kb on - strand, within Psyr_1325 at 1503.015 kb on - strand, within Psyr_1325 at 1503.015 kb on - strand, within Psyr_1325 at 1503.344 kb on - strand at 1503.489 kb on - strand, within Psyr_1326 at 1503.557 kb on + strand, within Psyr_1326 at 1503.557 kb on + strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.558 kb on - strand, within Psyr_1326 at 1503.600 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.721 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.862 kb on + strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1503.863 kb on - strand, within Psyr_1326 at 1504.108 kb on + strand at 1504.108 kb on + strand at 1504.109 kb on - strand at 1504.351 kb on - strand, within Psyr_1327 at 1504.388 kb on + strand, within Psyr_1327 at 1504.388 kb on + strand, within Psyr_1327 at 1504.388 kb on + strand, within Psyr_1327 at 1504.388 kb on + strand, within Psyr_1327 at 1504.388 kb on + strand, within Psyr_1327 at 1504.388 kb on + strand, within Psyr_1327 at 1504.389 kb on - strand, within Psyr_1327 at 1504.389 kb on - strand, within Psyr_1327 at 1504.424 kb on + strand, within Psyr_1327 at 1504.458 kb on + strand, within Psyr_1327 at 1504.568 kb on + strand, within Psyr_1327 at 1504.569 kb on - strand, within Psyr_1327 at 1504.569 kb on - strand, within Psyr_1327 at 1504.571 kb on + strand, within Psyr_1327 at 1504.591 kb on + strand at 1504.591 kb on + strand at 1504.591 kb on + strand at 1504.592 kb on - strand at 1504.595 kb on - strand at 1504.663 kb on + strand at 1504.664 kb on - strand at 1504.664 kb on - strand at 1504.664 kb on - strand at 1504.683 kb on - strand at 1504.695 kb on + strand at 1504.709 kb on + strand at 1504.864 kb on + strand at 1504.864 kb on + strand at 1504.865 kb on - strand at 1504.897 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Methyl-2-Hydroxy-Phenylproprionate 10 mM remove 1,502,389 + +0.8 1,502,400 - +1.2 1,502,430 - +0.2 1,502,479 - -1.7 1,503,014 + Psyr_1325 0.65 -0.1 1,503,014 + Psyr_1325 0.65 +0.7 1,503,015 - Psyr_1325 0.65 +0.7 1,503,015 - Psyr_1325 0.65 +0.1 1,503,015 - Psyr_1325 0.65 -0.8 1,503,015 - Psyr_1325 0.65 +0.5 1,503,344 - -1.1 1,503,489 - Psyr_1326 0.18 -0.3 1,503,557 + Psyr_1326 0.28 +0.8 1,503,557 + Psyr_1326 0.28 +1.2 1,503,558 - Psyr_1326 0.28 -0.4 1,503,558 - Psyr_1326 0.28 +1.7 1,503,558 - Psyr_1326 0.28 -1.6 1,503,558 - Psyr_1326 0.28 +0.3 1,503,600 + Psyr_1326 0.34 +0.6 1,503,721 + Psyr_1326 0.51 +1.5 1,503,721 + Psyr_1326 0.51 +0.0 1,503,721 + Psyr_1326 0.51 -1.1 1,503,721 + Psyr_1326 0.51 +0.5 1,503,721 + Psyr_1326 0.51 -0.4 1,503,721 + Psyr_1326 0.51 +0.0 1,503,721 + Psyr_1326 0.51 +2.3 1,503,862 + Psyr_1326 0.72 -0.1 1,503,862 + Psyr_1326 0.72 +0.0 1,503,862 + Psyr_1326 0.72 -1.0 1,503,862 + Psyr_1326 0.72 -0.9 1,503,862 + Psyr_1326 0.72 +0.7 1,503,863 - Psyr_1326 0.72 +1.2 1,503,863 - Psyr_1326 0.72 -0.7 1,503,863 - Psyr_1326 0.72 +0.6 1,503,863 - Psyr_1326 0.72 +2.2 1,503,863 - Psyr_1326 0.72 +0.7 1,504,108 + +1.2 1,504,108 + +0.2 1,504,109 - -2.3 1,504,351 - Psyr_1327 0.26 -0.2 1,504,388 + Psyr_1327 0.36 -0.1 1,504,388 + Psyr_1327 0.36 +0.4 1,504,388 + Psyr_1327 0.36 +1.0 1,504,388 + Psyr_1327 0.36 +0.1 1,504,388 + Psyr_1327 0.36 +1.4 1,504,388 + Psyr_1327 0.36 +0.6 1,504,389 - Psyr_1327 0.36 +0.4 1,504,389 - Psyr_1327 0.36 +1.0 1,504,424 + Psyr_1327 0.46 -1.6 1,504,458 + Psyr_1327 0.56 +0.3 1,504,568 + Psyr_1327 0.87 +0.3 1,504,569 - Psyr_1327 0.88 -0.3 1,504,569 - Psyr_1327 0.88 -0.6 1,504,571 + Psyr_1327 0.88 +0.9 1,504,591 + -1.3 1,504,591 + -0.8 1,504,591 + +0.8 1,504,592 - +0.4 1,504,595 - -0.0 1,504,663 + +0.6 1,504,664 - -1.4 1,504,664 - -0.5 1,504,664 - -0.4 1,504,683 - +0.6 1,504,695 + -1.6 1,504,709 + -0.5 1,504,864 + +0.4 1,504,864 + -0.5 1,504,865 - -1.0 1,504,897 - -0.6
Or see this region's nucleotide sequence