Experiment: LB Anaerobic with Tetracycline 0.0000003125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rrlH and rrfH are separated by 93 nucleotides rrfH and aspU are separated by 52 nucleotides aspU and dkgB are separated by 162 nucleotides dkgB and yafC overlap by 4 nucleotides
b0204: rrlH - 23S ribosomal RNA (NCBI), at 225,759 to 228,662
rrlH
b0205: rrfH - 5S ribosomal RNA (NCBI), at 228,756 to 228,875
rrfH
b0206: aspU - tRNA-Asp (NCBI), at 228,928 to 229,004
aspU
b0207: dkgB - 2,5-diketo-D-gluconate reductase B (NCBI), at 229,167 to 229,970
dkgB
b0208: yafC - predicted DNA-binding transcriptional regulator (NCBI), at 229,967 to 230,881
yafC
Position (kb) 229
230 Strain fitness (log2 ratio) -1
0
1 at 229.157 kb on - strand at 229.224 kb on - strand at 229.230 kb on - strand at 229.351 kb on - strand, within dkgB at 229.437 kb on - strand, within dkgB at 229.491 kb on - strand, within dkgB at 229.501 kb on - strand, within dkgB at 229.501 kb on - strand, within dkgB at 229.674 kb on - strand, within dkgB at 229.674 kb on - strand, within dkgB at 229.685 kb on + strand, within dkgB at 229.755 kb on + strand, within dkgB at 229.763 kb on - strand, within dkgB at 229.768 kb on - strand, within dkgB at 229.798 kb on + strand, within dkgB at 229.802 kb on + strand, within dkgB at 229.803 kb on + strand, within dkgB at 229.830 kb on + strand, within dkgB at 229.972 kb on - strand at 229.972 kb on - strand at 230.069 kb on - strand, within yafC at 230.208 kb on - strand, within yafC at 230.223 kb on - strand, within yafC at 230.230 kb on + strand, within yafC at 230.244 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.276 kb on - strand, within yafC at 230.282 kb on + strand, within yafC at 230.282 kb on + strand, within yafC at 230.323 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.549 kb on - strand, within yafC at 230.572 kb on + strand, within yafC at 230.600 kb on - strand, within yafC at 230.611 kb on + strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.732 kb on - strand, within yafC at 230.785 kb on - strand, within yafC at 230.785 kb on - strand, within yafC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Tetracycline 0.0000003125 mM remove 229,157 - +0.3 229,224 - +0.2 229,230 - +0.5 229,351 - dkgB b0207 0.23 +0.5 229,437 - dkgB b0207 0.34 +1.4 229,491 - dkgB b0207 0.40 -0.9 229,501 - dkgB b0207 0.42 -0.7 229,501 - dkgB b0207 0.42 -0.4 229,674 - dkgB b0207 0.63 -1.6 229,674 - dkgB b0207 0.63 -1.1 229,685 + dkgB b0207 0.64 +0.2 229,755 + dkgB b0207 0.73 +0.2 229,763 - dkgB b0207 0.74 +0.1 229,768 - dkgB b0207 0.75 -0.3 229,798 + dkgB b0207 0.78 -0.1 229,802 + dkgB b0207 0.79 +0.6 229,803 + dkgB b0207 0.79 +0.3 229,830 + dkgB b0207 0.82 -0.3 229,972 - +0.4 229,972 - +0.8 230,069 - yafC b0208 0.11 -1.1 230,208 - yafC b0208 0.26 +0.1 230,223 - yafC b0208 0.28 -0.2 230,230 + yafC b0208 0.29 +0.1 230,244 - yafC b0208 0.30 +0.1 230,276 - yafC b0208 0.34 +0.5 230,276 - yafC b0208 0.34 -0.9 230,282 + yafC b0208 0.34 -0.1 230,282 + yafC b0208 0.34 +0.7 230,323 - yafC b0208 0.39 +1.1 230,549 - yafC b0208 0.64 -0.8 230,549 - yafC b0208 0.64 -1.1 230,572 + yafC b0208 0.66 -0.4 230,600 - yafC b0208 0.69 +0.2 230,611 + yafC b0208 0.70 -0.7 230,732 - yafC b0208 0.84 -0.9 230,732 - yafC b0208 0.84 -1.4 230,732 - yafC b0208 0.84 +0.1 230,785 - yafC b0208 0.89 +0.5 230,785 - yafC b0208 0.89 -0.5
Or see this region's nucleotide sequence