Strain Fitness in Escherichia coli BW25113 around b3371

Experiment: LB Anaerobic with Amoxicillin 0.00000390625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyhfM and yhfN are separated by 20 nucleotidesyhfN and frlC are separated by 49 nucleotidesfrlC and frlD overlap by 4 nucleotides b3370: yhfM - putative amino acid/amine transport protein (VIMSS), at 3,497,932 to 3,499,269 yhfM b3371: yhfN - putative transport protein (VIMSS), at 3,499,290 to 3,500,312 yhfN b4474: frlC - predicted isomerase (NCBI), at 3,500,362 to 3,501,192 frlC b3374: frlD - fructoselysine 6-kinase (NCBI), at 3,501,189 to 3,501,974 frlD Position (kb) 3499 3500 3501Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3498.349 kb on + strand, within yhfMat 3498.351 kb on + strand, within yhfMat 3498.351 kb on + strand, within yhfMat 3498.365 kb on - strand, within yhfMat 3498.365 kb on - strand, within yhfMat 3498.365 kb on - strand, within yhfMat 3498.375 kb on - strand, within yhfMat 3498.385 kb on + strand, within yhfMat 3498.393 kb on - strand, within yhfMat 3498.446 kb on - strand, within yhfMat 3498.446 kb on - strand, within yhfMat 3498.517 kb on - strand, within yhfMat 3498.535 kb on + strand, within yhfMat 3498.543 kb on - strand, within yhfMat 3498.543 kb on - strand, within yhfMat 3498.549 kb on + strand, within yhfMat 3498.549 kb on + strand, within yhfMat 3498.575 kb on + strand, within yhfMat 3498.620 kb on - strand, within yhfMat 3498.620 kb on - strand, within yhfMat 3498.640 kb on - strand, within yhfMat 3498.655 kb on + strand, within yhfMat 3498.655 kb on + strand, within yhfMat 3498.655 kb on + strand, within yhfMat 3498.664 kb on + strand, within yhfMat 3498.664 kb on + strand, within yhfMat 3498.667 kb on - strand, within yhfMat 3498.667 kb on - strand, within yhfMat 3498.670 kb on - strand, within yhfMat 3498.749 kb on - strand, within yhfMat 3498.827 kb on - strand, within yhfMat 3498.827 kb on - strand, within yhfMat 3498.837 kb on - strand, within yhfMat 3498.840 kb on - strand, within yhfMat 3498.850 kb on + strand, within yhfMat 3498.850 kb on + strand, within yhfMat 3498.892 kb on + strand, within yhfMat 3498.892 kb on + strand, within yhfMat 3498.900 kb on - strand, within yhfMat 3498.941 kb on + strand, within yhfMat 3498.952 kb on + strand, within yhfMat 3498.966 kb on - strand, within yhfMat 3498.981 kb on + strand, within yhfMat 3498.988 kb on - strand, within yhfMat 3498.998 kb on + strand, within yhfMat 3498.998 kb on + strand, within yhfMat 3499.048 kb on + strand, within yhfMat 3499.048 kb on + strand, within yhfMat 3499.057 kb on + strand, within yhfMat 3499.085 kb on + strand, within yhfMat 3499.180 kb on - strandat 3499.213 kb on - strandat 3499.213 kb on - strandat 3499.213 kb on - strandat 3499.263 kb on + strandat 3499.263 kb on + strandat 3499.395 kb on + strand, within yhfNat 3499.398 kb on + strand, within yhfNat 3499.398 kb on + strand, within yhfNat 3499.401 kb on - strand, within yhfNat 3499.462 kb on - strand, within yhfNat 3499.508 kb on + strand, within yhfNat 3499.508 kb on + strand, within yhfNat 3499.532 kb on + strand, within yhfNat 3499.645 kb on - strand, within yhfNat 3499.664 kb on + strand, within yhfNat 3499.675 kb on + strand, within yhfNat 3499.762 kb on - strand, within yhfNat 3499.762 kb on - strand, within yhfNat 3499.767 kb on + strand, within yhfNat 3499.767 kb on + strand, within yhfNat 3499.775 kb on - strand, within yhfNat 3499.899 kb on - strand, within yhfNat 3499.914 kb on - strand, within yhfNat 3499.929 kb on - strand, within yhfNat 3499.935 kb on - strand, within yhfNat 3499.950 kb on + strand, within yhfNat 3499.982 kb on + strand, within yhfNat 3500.052 kb on + strand, within yhfNat 3500.136 kb on - strand, within yhfNat 3500.192 kb on + strand, within yhfNat 3500.237 kb on - strandat 3500.237 kb on - strandat 3500.239 kb on + strandat 3500.245 kb on - strandat 3500.318 kb on + strandat 3500.318 kb on + strandat 3500.364 kb on + strandat 3500.400 kb on + strandat 3500.437 kb on + strandat 3500.445 kb on - strand, within frlCat 3500.476 kb on - strand, within frlCat 3500.548 kb on + strand, within frlCat 3500.548 kb on + strand, within frlCat 3500.569 kb on + strand, within frlCat 3500.588 kb on + strand, within frlCat 3500.591 kb on - strand, within frlCat 3500.595 kb on - strand, within frlCat 3500.605 kb on + strand, within frlCat 3500.605 kb on + strand, within frlCat 3500.625 kb on - strand, within frlCat 3500.625 kb on - strand, within frlCat 3500.649 kb on + strand, within frlCat 3500.680 kb on - strand, within frlCat 3500.711 kb on - strand, within frlCat 3500.718 kb on + strand, within frlCat 3500.718 kb on + strand, within frlCat 3500.726 kb on - strand, within frlCat 3500.739 kb on + strand, within frlCat 3500.763 kb on + strand, within frlCat 3500.763 kb on + strand, within frlCat 3500.763 kb on + strand, within frlCat 3500.763 kb on + strand, within frlCat 3500.767 kb on - strand, within frlCat 3500.789 kb on + strand, within frlCat 3500.815 kb on - strand, within frlCat 3500.830 kb on + strand, within frlCat 3500.833 kb on + strand, within frlCat 3500.861 kb on + strand, within frlCat 3500.861 kb on - strand, within frlCat 3500.861 kb on - strand, within frlCat 3500.899 kb on + strand, within frlCat 3501.008 kb on - strand, within frlCat 3501.020 kb on - strand, within frlCat 3501.028 kb on - strand, within frlCat 3501.035 kb on + strand, within frlCat 3501.035 kb on + strand, within frlCat 3501.062 kb on + strand, within frlCat 3501.062 kb on + strand, within frlCat 3501.090 kb on + strand, within frlCat 3501.098 kb on - strand, within frlCat 3501.100 kb on - strand, within frlCat 3501.105 kb on + strand, within frlCat 3501.114 kb on + strandat 3501.114 kb on + strandat 3501.114 kb on + strandat 3501.123 kb on - strandat 3501.123 kb on - strandat 3501.125 kb on + strandat 3501.169 kb on + strandat 3501.169 kb on + strandat 3501.178 kb on + strandat 3501.239 kb on + strandat 3501.240 kb on - strandat 3501.284 kb on + strand, within frlDat 3501.292 kb on - strand, within frlD

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.00000390625 mM
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3,498,349 + yhfM b3370 0.31 +0.6
3,498,351 + yhfM b3370 0.31 +1.6
3,498,351 + yhfM b3370 0.31 -1.0
3,498,365 - yhfM b3370 0.32 -0.2
3,498,365 - yhfM b3370 0.32 +0.8
3,498,365 - yhfM b3370 0.32 +0.2
3,498,375 - yhfM b3370 0.33 +0.1
3,498,385 + yhfM b3370 0.34 +0.9
3,498,393 - yhfM b3370 0.34 +0.6
3,498,446 - yhfM b3370 0.38 -1.4
3,498,446 - yhfM b3370 0.38 -0.3
3,498,517 - yhfM b3370 0.44 +0.1
3,498,535 + yhfM b3370 0.45 +0.0
3,498,543 - yhfM b3370 0.46 -1.0
3,498,543 - yhfM b3370 0.46 -0.3
3,498,549 + yhfM b3370 0.46 +0.7
3,498,549 + yhfM b3370 0.46 -0.6
3,498,575 + yhfM b3370 0.48 -0.6
3,498,620 - yhfM b3370 0.51 -0.0
3,498,620 - yhfM b3370 0.51 -0.3
3,498,640 - yhfM b3370 0.53 +0.3
3,498,655 + yhfM b3370 0.54 +0.4
3,498,655 + yhfM b3370 0.54 +0.4
3,498,655 + yhfM b3370 0.54 +0.7
3,498,664 + yhfM b3370 0.55 -0.7
3,498,664 + yhfM b3370 0.55 -0.1
3,498,667 - yhfM b3370 0.55 -1.1
3,498,667 - yhfM b3370 0.55 -0.1
3,498,670 - yhfM b3370 0.55 -1.0
3,498,749 - yhfM b3370 0.61 -1.1
3,498,827 - yhfM b3370 0.67 +0.1
3,498,827 - yhfM b3370 0.67 +0.1
3,498,837 - yhfM b3370 0.68 +0.4
3,498,840 - yhfM b3370 0.68 +0.0
3,498,850 + yhfM b3370 0.69 -1.6
3,498,850 + yhfM b3370 0.69 -0.6
3,498,892 + yhfM b3370 0.72 -0.6
3,498,892 + yhfM b3370 0.72 -0.1
3,498,900 - yhfM b3370 0.72 -0.1
3,498,941 + yhfM b3370 0.75 -0.3
3,498,952 + yhfM b3370 0.76 +0.5
3,498,966 - yhfM b3370 0.77 -0.2
3,498,981 + yhfM b3370 0.78 -0.1
3,498,988 - yhfM b3370 0.79 +1.7
3,498,998 + yhfM b3370 0.80 +0.7
3,498,998 + yhfM b3370 0.80 +0.4
3,499,048 + yhfM b3370 0.83 -0.3
3,499,048 + yhfM b3370 0.83 -0.3
3,499,057 + yhfM b3370 0.84 +0.4
3,499,085 + yhfM b3370 0.86 +0.5
3,499,180 - +0.2
3,499,213 - -0.7
3,499,213 - -0.4
3,499,213 - -1.4
3,499,263 + -0.0
3,499,263 + +0.3
3,499,395 + yhfN b3371 0.10 +0.9
3,499,398 + yhfN b3371 0.11 +3.0
3,499,398 + yhfN b3371 0.11 -0.8
3,499,401 - yhfN b3371 0.11 -0.4
3,499,462 - yhfN b3371 0.17 -0.2
3,499,508 + yhfN b3371 0.21 +0.3
3,499,508 + yhfN b3371 0.21 +1.0
3,499,532 + yhfN b3371 0.24 +1.0
3,499,645 - yhfN b3371 0.35 -0.2
3,499,664 + yhfN b3371 0.37 +0.4
3,499,675 + yhfN b3371 0.38 -0.3
3,499,762 - yhfN b3371 0.46 -2.3
3,499,762 - yhfN b3371 0.46 +0.1
3,499,767 + yhfN b3371 0.47 +1.2
3,499,767 + yhfN b3371 0.47 -0.1
3,499,775 - yhfN b3371 0.47 +0.0
3,499,899 - yhfN b3371 0.60 +0.3
3,499,914 - yhfN b3371 0.61 +0.7
3,499,929 - yhfN b3371 0.62 +0.9
3,499,935 - yhfN b3371 0.63 +0.0
3,499,950 + yhfN b3371 0.65 -1.0
3,499,982 + yhfN b3371 0.68 +0.0
3,500,052 + yhfN b3371 0.74 +0.4
3,500,136 - yhfN b3371 0.83 +0.3
3,500,192 + yhfN b3371 0.88 +0.1
3,500,237 - -0.8
3,500,237 - +1.3
3,500,239 + -0.1
3,500,245 - -1.8
3,500,318 + -0.9
3,500,318 + +0.4
3,500,364 + -0.1
3,500,400 + -0.0
3,500,437 + -1.0
3,500,445 - frlC b4474 0.10 -0.6
3,500,476 - frlC b4474 0.14 -0.0
3,500,548 + frlC b4474 0.22 +0.7
3,500,548 + frlC b4474 0.22 +0.1
3,500,569 + frlC b4474 0.25 -0.1
3,500,588 + frlC b4474 0.27 -0.6
3,500,591 - frlC b4474 0.28 +0.6
3,500,595 - frlC b4474 0.28 -1.2
3,500,605 + frlC b4474 0.29 +0.7
3,500,605 + frlC b4474 0.29 -0.6
3,500,625 - frlC b4474 0.32 +2.0
3,500,625 - frlC b4474 0.32 +0.2
3,500,649 + frlC b4474 0.35 -0.4
3,500,680 - frlC b4474 0.38 -0.1
3,500,711 - frlC b4474 0.42 +0.9
3,500,718 + frlC b4474 0.43 +0.0
3,500,718 + frlC b4474 0.43 +0.1
3,500,726 - frlC b4474 0.44 -1.5
3,500,739 + frlC b4474 0.45 +0.3
3,500,763 + frlC b4474 0.48 -0.4
3,500,763 + frlC b4474 0.48 +1.0
3,500,763 + frlC b4474 0.48 -0.2
3,500,763 + frlC b4474 0.48 +0.3
3,500,767 - frlC b4474 0.49 +0.0
3,500,789 + frlC b4474 0.51 +0.6
3,500,815 - frlC b4474 0.55 +0.2
3,500,830 + frlC b4474 0.56 -0.2
3,500,833 + frlC b4474 0.57 +0.3
3,500,861 + frlC b4474 0.60 -0.0
3,500,861 - frlC b4474 0.60 -0.9
3,500,861 - frlC b4474 0.60 +0.2
3,500,899 + frlC b4474 0.65 +0.4
3,501,008 - frlC b4474 0.78 -0.5
3,501,020 - frlC b4474 0.79 +0.2
3,501,028 - frlC b4474 0.80 +0.4
3,501,035 + frlC b4474 0.81 +1.1
3,501,035 + frlC b4474 0.81 -0.0
3,501,062 + frlC b4474 0.84 -0.2
3,501,062 + frlC b4474 0.84 +0.8
3,501,090 + frlC b4474 0.88 +0.0
3,501,098 - frlC b4474 0.89 +0.7
3,501,100 - frlC b4474 0.89 +0.5
3,501,105 + frlC b4474 0.89 -0.5
3,501,114 + +0.1
3,501,114 + +0.6
3,501,114 + +0.3
3,501,123 - +1.0
3,501,123 - +0.0
3,501,125 + +0.5
3,501,169 + +0.2
3,501,169 + -0.2
3,501,178 + +1.6
3,501,239 + -0.4
3,501,240 - -0.6
3,501,284 + frlD b3374 0.12 -0.4
3,501,292 - frlD b3374 0.13 +0.5

Or see this region's nucleotide sequence