Experiment: LB Anaerobic with Amoxicillin 0.00000390625 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nadC and ampD are separated by 87 nucleotides ampD and ampE overlap by 4 nucleotides ampE and aroP are separated by 42 nucleotides
b0109: nadC - nicotinate-nucleotide pyrophosphorylase (NCBI), at 117,752 to 118,645
nadC
b0110: ampD - N-acetyl-anhydromuranmyl-L-alanine amidase (NCBI), at 118,733 to 119,284
ampD
b0111: ampE - predicted inner membrane protein (NCBI), at 119,281 to 120,135
ampE
b0112: aroP - aromatic amino acid transporter (NCBI), at 120,178 to 121,551
aroP
Position (kb)
118
119
120 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 117.839 kb on + strand at 117.856 kb on - strand, within nadC at 117.856 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.879 kb on - strand, within nadC at 117.881 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 117.922 kb on - strand, within nadC at 118.107 kb on - strand, within nadC at 118.201 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.230 kb on + strand, within nadC at 118.296 kb on + strand, within nadC at 118.355 kb on - strand, within nadC at 118.355 kb on - strand, within nadC at 118.364 kb on + strand, within nadC at 118.382 kb on - strand, within nadC at 118.538 kb on + strand, within nadC at 118.623 kb on + strand at 118.631 kb on - strand at 118.737 kb on + strand at 118.737 kb on + strand at 118.781 kb on + strand at 118.836 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.868 kb on + strand, within ampD at 118.904 kb on - strand, within ampD at 119.033 kb on - strand, within ampD at 119.101 kb on + strand, within ampD at 119.109 kb on - strand, within ampD at 119.109 kb on - strand, within ampD at 119.117 kb on - strand, within ampD at 119.119 kb on + strand, within ampD at 119.150 kb on - strand, within ampD at 119.150 kb on - strand, within ampD at 119.163 kb on + strand, within ampD at 119.168 kb on - strand, within ampD at 119.191 kb on + strand, within ampD at 119.191 kb on + strand, within ampD at 119.274 kb on + strand at 119.339 kb on + strand at 119.339 kb on + strand at 119.499 kb on - strand, within ampE at 119.499 kb on - strand, within ampE at 119.522 kb on + strand, within ampE at 119.528 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.555 kb on - strand, within ampE at 119.558 kb on - strand, within ampE at 119.567 kb on + strand, within ampE at 119.567 kb on + strand, within ampE at 119.753 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.755 kb on - strand, within ampE at 119.756 kb on + strand, within ampE at 119.772 kb on - strand, within ampE at 119.786 kb on + strand, within ampE at 119.786 kb on + strand, within ampE at 119.799 kb on - strand, within ampE at 119.799 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.836 kb on - strand, within ampE at 119.915 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.961 kb on + strand, within ampE at 119.983 kb on + strand, within ampE at 119.985 kb on - strand, within ampE at 120.006 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.013 kb on - strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.014 kb on + strand, within ampE at 120.037 kb on - strand, within ampE at 120.138 kb on - strand at 120.139 kb on + strand at 120.153 kb on - strand at 120.155 kb on + strand at 120.237 kb on - strand at 120.251 kb on - strand at 120.254 kb on + strand at 120.266 kb on - strand at 120.266 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.00000390625 mM remove 117,839 + -0.4 117,856 - nadC b0109 0.12 -0.1 117,856 - nadC b0109 0.12 -0.2 117,879 - nadC b0109 0.14 -0.8 117,879 - nadC b0109 0.14 +0.5 117,881 - nadC b0109 0.14 -1.1 117,922 - nadC b0109 0.19 +1.2 117,922 - nadC b0109 0.19 -0.4 118,107 - nadC b0109 0.40 -0.0 118,201 + nadC b0109 0.50 +0.7 118,230 + nadC b0109 0.53 +0.4 118,230 + nadC b0109 0.53 -0.1 118,296 + nadC b0109 0.61 +0.2 118,355 - nadC b0109 0.67 -0.2 118,355 - nadC b0109 0.67 -0.1 118,364 + nadC b0109 0.68 -0.1 118,382 - nadC b0109 0.70 -1.1 118,538 + nadC b0109 0.88 +0.8 118,623 + +0.5 118,631 - -0.5 118,737 + +0.1 118,737 + +0.5 118,781 + -0.8 118,836 + ampD b0110 0.19 -0.4 118,868 + ampD b0110 0.24 -1.4 118,868 + ampD b0110 0.24 +0.8 118,904 - ampD b0110 0.31 -0.4 119,033 - ampD b0110 0.54 -0.9 119,101 + ampD b0110 0.67 -0.5 119,109 - ampD b0110 0.68 -1.4 119,109 - ampD b0110 0.68 -0.5 119,117 - ampD b0110 0.70 -1.5 119,119 + ampD b0110 0.70 -2.0 119,150 - ampD b0110 0.76 -1.0 119,150 - ampD b0110 0.76 +0.3 119,163 + ampD b0110 0.78 -1.3 119,168 - ampD b0110 0.79 -0.5 119,191 + ampD b0110 0.83 -1.3 119,191 + ampD b0110 0.83 -2.2 119,274 + +0.7 119,339 + +1.0 119,339 + +2.0 119,499 - ampE b0111 0.25 -1.0 119,499 - ampE b0111 0.25 +0.4 119,522 + ampE b0111 0.28 +1.0 119,528 - ampE b0111 0.29 -0.3 119,555 - ampE b0111 0.32 -0.9 119,555 - ampE b0111 0.32 -0.2 119,558 - ampE b0111 0.32 -0.4 119,567 + ampE b0111 0.33 +0.1 119,567 + ampE b0111 0.33 +1.0 119,753 - ampE b0111 0.55 +0.0 119,755 - ampE b0111 0.55 -0.7 119,755 - ampE b0111 0.55 -0.2 119,756 + ampE b0111 0.56 -0.2 119,772 - ampE b0111 0.57 -0.3 119,786 + ampE b0111 0.59 +1.0 119,786 + ampE b0111 0.59 +0.8 119,799 - ampE b0111 0.61 -0.2 119,799 - ampE b0111 0.61 -1.3 119,836 - ampE b0111 0.65 -2.8 119,836 - ampE b0111 0.65 -0.5 119,915 + ampE b0111 0.74 -0.0 119,961 + ampE b0111 0.80 -0.8 119,961 + ampE b0111 0.80 +0.8 119,983 + ampE b0111 0.82 -0.2 119,985 - ampE b0111 0.82 +0.7 120,006 - ampE b0111 0.85 +0.5 120,013 - ampE b0111 0.86 -0.5 120,013 - ampE b0111 0.86 +1.0 120,014 + ampE b0111 0.86 +0.1 120,014 + ampE b0111 0.86 +0.3 120,014 + ampE b0111 0.86 +0.5 120,014 + ampE b0111 0.86 -1.8 120,037 - ampE b0111 0.88 -0.2 120,138 - -0.4 120,139 + -0.1 120,153 - +0.6 120,155 + -0.4 120,237 - -0.6 120,251 - -0.1 120,254 + -0.7 120,266 - +0.0 120,266 - -0.1
Or see this region's nucleotide sequence