Strain Fitness in Escherichia coli BW25113 around b3189

Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntispB and sfsB are separated by 227 nucleotidessfsB and murA are separated by 47 nucleotidesmurA and yrbA are separated by 54 nucleotidesyrbA and yrbB are separated by 159 nucleotidesyrbB and yrbC overlap by 1 nucleotides b3187: ispB - octaprenyl diphosphate synthase (NCBI), at 3,331,732 to 3,332,703 ispB b3188: sfsB - DNA-binding transcriptional activator of maltose metabolism (NCBI), at 3,332,931 to 3,333,209 sfsB b3189: murA - UDP-N-acetylglucosamine 1-carboxyvinyltransferase (NCBI), at 3,333,257 to 3,334,516 murA b3190: yrbA - orf, hypothetical protein (VIMSS), at 3,334,571 to 3,334,825 yrbA b3191: yrbB - orf, hypothetical protein (VIMSS), at 3,334,985 to 3,335,278 yrbB b3192: yrbC - predicted ABC-type organic solvent transporter (NCBI), at 3,335,278 to 3,335,913 yrbC Position (kb) 3333 3334 3335Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3332.691 kb on - strandat 3332.718 kb on - strandat 3332.858 kb on - strandat 3332.858 kb on - strandat 3332.866 kb on + strandat 3332.913 kb on + strandat 3332.919 kb on - strandat 3333.104 kb on - strand, within sfsBat 3333.104 kb on - strand, within sfsBat 3333.144 kb on + strand, within sfsBat 3333.150 kb on + strand, within sfsBat 3333.151 kb on + strand, within sfsBat 3333.176 kb on + strand, within sfsBat 3333.190 kb on - strandat 3334.619 kb on - strand, within yrbAat 3334.630 kb on + strand, within yrbAat 3334.638 kb on - strand, within yrbAat 3334.638 kb on - strand, within yrbAat 3334.639 kb on + strand, within yrbAat 3334.639 kb on + strand, within yrbAat 3334.710 kb on - strand, within yrbAat 3334.718 kb on + strand, within yrbAat 3334.724 kb on + strand, within yrbAat 3334.780 kb on - strand, within yrbAat 3334.780 kb on - strand, within yrbAat 3334.793 kb on + strand, within yrbAat 3334.801 kb on - strandat 3334.801 kb on - strandat 3334.843 kb on + strandat 3334.883 kb on + strandat 3334.918 kb on + strandat 3334.918 kb on + strandat 3335.030 kb on + strand, within yrbBat 3335.030 kb on + strand, within yrbBat 3335.030 kb on + strand, within yrbBat 3335.030 kb on + strand, within yrbBat 3335.030 kb on + strand, within yrbBat 3335.030 kb on + strand, within yrbBat 3335.031 kb on + strand, within yrbBat 3335.031 kb on + strand, within yrbBat 3335.036 kb on + strand, within yrbBat 3335.036 kb on + strand, within yrbBat 3335.038 kb on - strand, within yrbBat 3335.038 kb on - strand, within yrbBat 3335.038 kb on - strand, within yrbBat 3335.038 kb on - strand, within yrbBat 3335.038 kb on - strand, within yrbBat 3335.059 kb on - strand, within yrbBat 3335.060 kb on - strand, within yrbBat 3335.098 kb on + strand, within yrbBat 3335.098 kb on + strand, within yrbBat 3335.102 kb on + strand, within yrbBat 3335.102 kb on + strand, within yrbBat 3335.120 kb on + strand, within yrbBat 3335.128 kb on - strand, within yrbBat 3335.137 kb on + strand, within yrbBat 3335.142 kb on + strand, within yrbBat 3335.142 kb on + strand, within yrbBat 3335.162 kb on - strand, within yrbBat 3335.165 kb on + strand, within yrbBat 3335.198 kb on + strand, within yrbBat 3335.198 kb on + strand, within yrbBat 3335.205 kb on + strand, within yrbBat 3335.236 kb on - strand, within yrbBat 3335.340 kb on + strandat 3335.362 kb on - strand, within yrbCat 3335.362 kb on - strand, within yrbCat 3335.404 kb on + strand, within yrbCat 3335.428 kb on - strand, within yrbCat 3335.428 kb on - strand, within yrbCat 3335.461 kb on + strand, within yrbCat 3335.481 kb on + strand, within yrbCat 3335.500 kb on + strand, within yrbCat 3335.500 kb on + strand, within yrbC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM
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3,332,691 - +3.5
3,332,718 - +0.8
3,332,858 - -1.1
3,332,858 - -0.5
3,332,866 + +0.2
3,332,913 + -1.9
3,332,919 - -0.1
3,333,104 - sfsB b3188 0.62 +0.5
3,333,104 - sfsB b3188 0.62 +0.2
3,333,144 + sfsB b3188 0.76 -0.9
3,333,150 + sfsB b3188 0.78 -0.7
3,333,151 + sfsB b3188 0.79 -0.8
3,333,176 + sfsB b3188 0.88 -0.5
3,333,190 - -1.6
3,334,619 - yrbA b3190 0.19 +0.4
3,334,630 + yrbA b3190 0.23 -0.5
3,334,638 - yrbA b3190 0.26 -0.4
3,334,638 - yrbA b3190 0.26 +0.6
3,334,639 + yrbA b3190 0.27 +0.1
3,334,639 + yrbA b3190 0.27 -0.3
3,334,710 - yrbA b3190 0.55 +0.5
3,334,718 + yrbA b3190 0.58 -0.8
3,334,724 + yrbA b3190 0.60 -0.6
3,334,780 - yrbA b3190 0.82 +1.5
3,334,780 - yrbA b3190 0.82 +0.1
3,334,793 + yrbA b3190 0.87 +0.4
3,334,801 - +0.7
3,334,801 - -1.4
3,334,843 + -2.6
3,334,883 + -0.2
3,334,918 + -1.7
3,334,918 + -0.3
3,335,030 + yrbB b3191 0.15 -1.4
3,335,030 + yrbB b3191 0.15 -0.5
3,335,030 + yrbB b3191 0.15 +0.3
3,335,030 + yrbB b3191 0.15 +1.7
3,335,030 + yrbB b3191 0.15 +0.6
3,335,030 + yrbB b3191 0.15 +0.4
3,335,031 + yrbB b3191 0.16 -0.3
3,335,031 + yrbB b3191 0.16 -0.1
3,335,036 + yrbB b3191 0.17 -1.4
3,335,036 + yrbB b3191 0.17 +0.5
3,335,038 - yrbB b3191 0.18 -0.9
3,335,038 - yrbB b3191 0.18 +0.3
3,335,038 - yrbB b3191 0.18 -0.6
3,335,038 - yrbB b3191 0.18 -0.2
3,335,038 - yrbB b3191 0.18 -0.4
3,335,059 - yrbB b3191 0.25 +0.3
3,335,060 - yrbB b3191 0.26 -1.1
3,335,098 + yrbB b3191 0.38 -1.9
3,335,098 + yrbB b3191 0.38 +0.7
3,335,102 + yrbB b3191 0.40 -1.2
3,335,102 + yrbB b3191 0.40 -0.9
3,335,120 + yrbB b3191 0.46 -0.6
3,335,128 - yrbB b3191 0.49 -1.2
3,335,137 + yrbB b3191 0.52 -1.9
3,335,142 + yrbB b3191 0.53 -0.1
3,335,142 + yrbB b3191 0.53 -1.3
3,335,162 - yrbB b3191 0.60 -0.9
3,335,165 + yrbB b3191 0.61 -2.3
3,335,198 + yrbB b3191 0.72 -0.9
3,335,198 + yrbB b3191 0.72 -0.3
3,335,205 + yrbB b3191 0.75 +1.7
3,335,236 - yrbB b3191 0.85 +0.1
3,335,340 + -1.5
3,335,362 - yrbC b3192 0.13 -1.5
3,335,362 - yrbC b3192 0.13 +0.4
3,335,404 + yrbC b3192 0.20 -0.3
3,335,428 - yrbC b3192 0.24 -0.9
3,335,428 - yrbC b3192 0.24 -0.7
3,335,461 + yrbC b3192 0.29 +0.7
3,335,481 + yrbC b3192 0.32 -2.2
3,335,500 + yrbC b3192 0.35 +0.1
3,335,500 + yrbC b3192 0.35 -0.2

Or see this region's nucleotide sequence