Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt recB and ptr overlap by 8 nucleotides ptr and recC are separated by 175 nucleotides
b2820: recB - exonuclease V (RecBCD complex), beta subunit (NCBI), at 2,950,483 to 2,954,025
recB
b2821: ptr - protease III (NCBI), at 2,954,018 to 2,956,906
ptr
b2822: recC - exonuclease V (RecBCD complex), gamma chain (NCBI), at 2,957,082 to 2,960,450
recC
Position (kb)
2954
2955
2956
2957 Strain fitness (log2 ratio)
-2
-1
0
1 at 2953.040 kb on - strand, within recB at 2953.978 kb on + strand at 2953.978 kb on + strand at 2954.006 kb on - strand at 2954.072 kb on + strand at 2954.072 kb on + strand at 2954.201 kb on - strand at 2954.359 kb on + strand, within ptr at 2954.508 kb on + strand, within ptr at 2954.516 kb on + strand, within ptr at 2954.587 kb on - strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.617 kb on + strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.626 kb on - strand, within ptr at 2954.713 kb on - strand, within ptr at 2954.724 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.759 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.771 kb on - strand, within ptr at 2954.777 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.782 kb on - strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.857 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.905 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr at 2954.985 kb on + strand, within ptr at 2955.059 kb on + strand, within ptr at 2955.080 kb on - strand, within ptr at 2955.090 kb on + strand, within ptr at 2955.232 kb on - strand, within ptr at 2955.232 kb on - strand, within ptr at 2955.232 kb on - strand, within ptr at 2955.265 kb on - strand, within ptr at 2955.334 kb on + strand, within ptr at 2955.413 kb on - strand, within ptr at 2955.413 kb on - strand, within ptr at 2955.482 kb on - strand, within ptr at 2955.573 kb on + strand, within ptr at 2955.733 kb on + strand, within ptr at 2955.842 kb on - strand, within ptr at 2955.842 kb on - strand, within ptr at 2955.842 kb on - strand, within ptr at 2955.907 kb on + strand, within ptr at 2955.907 kb on + strand, within ptr at 2955.912 kb on + strand, within ptr at 2955.940 kb on + strand, within ptr at 2956.077 kb on - strand, within ptr at 2956.077 kb on - strand, within ptr at 2956.082 kb on - strand, within ptr at 2956.082 kb on - strand, within ptr at 2956.353 kb on - strand, within ptr at 2956.360 kb on + strand, within ptr at 2956.362 kb on + strand, within ptr at 2956.370 kb on - strand, within ptr at 2956.419 kb on - strand, within ptr at 2956.478 kb on - strand, within ptr at 2956.478 kb on - strand, within ptr at 2956.485 kb on + strand, within ptr at 2956.485 kb on + strand, within ptr at 2956.598 kb on - strand, within ptr at 2956.607 kb on - strand, within ptr at 2956.653 kb on + strand at 2956.658 kb on - strand at 2956.658 kb on - strand at 2956.663 kb on + strand at 2956.772 kb on - strand at 2956.772 kb on - strand at 2956.844 kb on + strand at 2956.882 kb on - strand at 2957.218 kb on - strand at 2957.288 kb on + strand at 2957.823 kb on + strand, within recC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 2,953,040 - recB b2820 0.72 +0.6 2,953,978 + +0.6 2,953,978 + -0.4 2,954,006 - -0.4 2,954,072 + -0.6 2,954,072 + -0.2 2,954,201 - -1.4 2,954,359 + ptr b2821 0.12 -0.1 2,954,508 + ptr b2821 0.17 -1.2 2,954,516 + ptr b2821 0.17 -0.7 2,954,587 - ptr b2821 0.20 -1.2 2,954,617 + ptr b2821 0.21 -0.2 2,954,617 + ptr b2821 0.21 -0.1 2,954,626 - ptr b2821 0.21 -2.2 2,954,626 - ptr b2821 0.21 +0.3 2,954,713 - ptr b2821 0.24 -0.8 2,954,724 - ptr b2821 0.24 -0.2 2,954,759 - ptr b2821 0.26 +0.8 2,954,759 - ptr b2821 0.26 -0.4 2,954,771 - ptr b2821 0.26 +1.1 2,954,771 - ptr b2821 0.26 -0.2 2,954,777 - ptr b2821 0.26 -0.9 2,954,782 - ptr b2821 0.26 -0.6 2,954,782 - ptr b2821 0.26 +0.3 2,954,857 + ptr b2821 0.29 -1.3 2,954,857 + ptr b2821 0.29 -0.9 2,954,905 + ptr b2821 0.31 +1.3 2,954,905 + ptr b2821 0.31 -0.4 2,954,985 + ptr b2821 0.33 -1.3 2,954,985 + ptr b2821 0.33 -0.8 2,955,059 + ptr b2821 0.36 -1.1 2,955,080 - ptr b2821 0.37 -1.9 2,955,090 + ptr b2821 0.37 -1.2 2,955,232 - ptr b2821 0.42 -0.2 2,955,232 - ptr b2821 0.42 +0.6 2,955,232 - ptr b2821 0.42 -0.8 2,955,265 - ptr b2821 0.43 -1.0 2,955,334 + ptr b2821 0.46 -0.5 2,955,413 - ptr b2821 0.48 +1.6 2,955,413 - ptr b2821 0.48 -1.1 2,955,482 - ptr b2821 0.51 +0.2 2,955,573 + ptr b2821 0.54 +0.3 2,955,733 + ptr b2821 0.59 -1.6 2,955,842 - ptr b2821 0.63 -0.8 2,955,842 - ptr b2821 0.63 -0.8 2,955,842 - ptr b2821 0.63 -1.5 2,955,907 + ptr b2821 0.65 +0.1 2,955,907 + ptr b2821 0.65 -1.6 2,955,912 + ptr b2821 0.66 -0.2 2,955,940 + ptr b2821 0.67 -1.4 2,956,077 - ptr b2821 0.71 -0.8 2,956,077 - ptr b2821 0.71 +0.2 2,956,082 - ptr b2821 0.71 +0.3 2,956,082 - ptr b2821 0.71 +0.1 2,956,353 - ptr b2821 0.81 +1.1 2,956,360 + ptr b2821 0.81 -0.8 2,956,362 + ptr b2821 0.81 -0.2 2,956,370 - ptr b2821 0.81 -1.3 2,956,419 - ptr b2821 0.83 -0.4 2,956,478 - ptr b2821 0.85 -0.2 2,956,478 - ptr b2821 0.85 -2.3 2,956,485 + ptr b2821 0.85 -1.3 2,956,485 + ptr b2821 0.85 -1.9 2,956,598 - ptr b2821 0.89 -1.0 2,956,607 - ptr b2821 0.90 -0.6 2,956,653 + +0.0 2,956,658 - -0.4 2,956,658 - +0.6 2,956,663 + -0.4 2,956,772 - +0.3 2,956,772 - +0.5 2,956,844 + +0.2 2,956,882 - -0.1 2,957,218 - +1.6 2,957,288 + +1.6 2,957,823 + recC b2822 0.22 +1.6
Or see this region's nucleotide sequence