Strain Fitness in Escherichia coli BW25113 around b2316

Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntfolC and accD are separated by 69 nucleotidesaccD and dedA are separated by 155 nucleotidesdedA and truA are separated by 82 nucleotides b2315: folC - bifunctional folylpolyglutamate synthase/ dihydrofolate synthase (NCBI), at 2,429,696 to 2,430,964 folC b2316: accD - acetyl-CoA carboxylase subunit beta (NCBI), at 2,431,034 to 2,431,948 accD b2317: dedA - conserved inner membrane protein (NCBI), at 2,432,104 to 2,432,763 dedA b2318: truA - tRNA pseudouridine synthase A (NCBI), at 2,432,846 to 2,433,658 truA Position (kb) 2431 2432Strain fitness (log2 ratio) -2 -1 0 1 2at 2430.901 kb on - strandat 2430.958 kb on - strandat 2430.998 kb on - strandat 2430.998 kb on - strandat 2431.014 kb on - strandat 2431.015 kb on - strandat 2431.015 kb on - strandat 2431.016 kb on - strandat 2432.025 kb on - strandat 2432.025 kb on - strandat 2432.196 kb on + strand, within dedAat 2432.269 kb on - strand, within dedAat 2432.269 kb on - strand, within dedAat 2432.290 kb on - strand, within dedAat 2432.290 kb on - strand, within dedAat 2432.494 kb on + strand, within dedAat 2432.650 kb on + strand, within dedAat 2432.650 kb on + strand, within dedAat 2432.800 kb on + strandat 2432.808 kb on - strandat 2432.808 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM
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2,430,901 - +0.8
2,430,958 - +1.0
2,430,998 - -1.6
2,430,998 - -0.6
2,431,014 - +1.2
2,431,015 - +0.2
2,431,015 - +1.5
2,431,016 - +0.1
2,432,025 - -0.2
2,432,025 - -0.4
2,432,196 + dedA b2317 0.14 -0.1
2,432,269 - dedA b2317 0.25 +0.6
2,432,269 - dedA b2317 0.25 -0.5
2,432,290 - dedA b2317 0.28 +0.5
2,432,290 - dedA b2317 0.28 +1.0
2,432,494 + dedA b2317 0.59 -1.3
2,432,650 + dedA b2317 0.83 -2.1
2,432,650 + dedA b2317 0.83 +1.5
2,432,800 + +0.2
2,432,808 - -0.8
2,432,808 - +1.9

Or see this region's nucleotide sequence