Strain Fitness in Escherichia coli BW25113 around b1852

Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntedd and zwf are separated by 234 nucleotideszwf and yebK are separated by 337 nucleotides b1851: edd - phosphogluconate dehydratase (NCBI), at 1,930,817 to 1,932,628 edd b1852: zwf - glucose-6-phosphate 1-dehydrogenase (NCBI), at 1,932,863 to 1,934,338 zwf b1853: yebK - predicted DNA-binding transcriptional regulator (NCBI), at 1,934,676 to 1,935,545 yebK Position (kb) 1932 1933 1934 1935Strain fitness (log2 ratio) -1 0 1 2 3 4at 1931.924 kb on + strand, within eddat 1931.982 kb on + strand, within eddat 1931.982 kb on + strand, within eddat 1932.174 kb on + strand, within eddat 1932.174 kb on + strand, within eddat 1932.197 kb on - strand, within eddat 1932.197 kb on - strand, within eddat 1932.288 kb on - strand, within eddat 1932.379 kb on - strand, within eddat 1932.379 kb on - strand, within eddat 1932.383 kb on - strand, within eddat 1932.463 kb on - strandat 1932.468 kb on + strandat 1932.557 kb on + strandat 1932.557 kb on + strandat 1932.712 kb on - strandat 1932.959 kb on + strandat 1932.959 kb on + strandat 1933.633 kb on - strand, within zwfat 1934.272 kb on + strandat 1934.305 kb on + strandat 1934.347 kb on - strandat 1934.347 kb on - strandat 1934.373 kb on + strandat 1934.409 kb on + strandat 1934.587 kb on + strandat 1934.587 kb on + strandat 1934.818 kb on + strand, within yebKat 1934.893 kb on + strand, within yebK

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM
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1,931,924 + edd b1851 0.61 -1.7
1,931,982 + edd b1851 0.64 +0.7
1,931,982 + edd b1851 0.64 -0.3
1,932,174 + edd b1851 0.75 +0.7
1,932,174 + edd b1851 0.75 +1.5
1,932,197 - edd b1851 0.76 -1.7
1,932,197 - edd b1851 0.76 -1.3
1,932,288 - edd b1851 0.81 +0.7
1,932,379 - edd b1851 0.86 -0.0
1,932,379 - edd b1851 0.86 +2.1
1,932,383 - edd b1851 0.86 -0.3
1,932,463 - -0.3
1,932,468 + -0.5
1,932,557 + +4.2
1,932,557 + +1.8
1,932,712 - -0.7
1,932,959 + +0.3
1,932,959 + +1.3
1,933,633 - zwf b1852 0.52 +1.3
1,934,272 + -0.7
1,934,305 + +0.3
1,934,347 - +1.8
1,934,347 - -0.5
1,934,373 + -1.0
1,934,409 + -0.9
1,934,587 + -0.1
1,934,587 + +1.7
1,934,818 + yebK b1853 0.16 -0.7
1,934,893 + yebK b1853 0.25 -0.5

Or see this region's nucleotide sequence