Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt manY and manZ are separated by 12 nucleotides manZ and yobD are separated by 54 nucleotides yobD and b1821 are separated by 428 nucleotides b1821 and rrmA overlap by 4 nucleotides
b1818: manY - mannose-specific enzyme IIC component of PTS (NCBI), at 1,901,106 to 1,901,906
manY
b1819: manZ - mannose-specific enzyme IID component of PTS (NCBI), at 1,901,919 to 1,902,770
manZ
b1820: yobD - hypothetical protein (NCBI), at 1,902,825 to 1,903,283
yobD
b1821: b1821 - orf, hypothetical protein (VIMSS), at 1,903,712 to 1,904,278
b1821
b1822: rrmA - 23S rRNA m1G745 methyltransferase (NCBI), at 1,904,275 to 1,905,084
rrmA
Position (kb)
1902
1903
1904 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1902.131 kb on + strand, within manZ at 1902.131 kb on + strand, within manZ at 1902.139 kb on - strand, within manZ at 1902.139 kb on - strand, within manZ at 1902.146 kb on + strand, within manZ at 1902.425 kb on - strand, within manZ at 1902.428 kb on + strand, within manZ at 1902.618 kb on + strand, within manZ at 1902.618 kb on + strand, within manZ at 1902.626 kb on - strand, within manZ at 1902.758 kb on + strand at 1902.763 kb on + strand at 1902.765 kb on + strand at 1902.765 kb on - strand at 1902.766 kb on - strand at 1902.773 kb on - strand at 1902.781 kb on - strand at 1902.867 kb on + strand at 1902.867 kb on + strand at 1902.875 kb on - strand, within yobD at 1902.875 kb on - strand at 1902.875 kb on - strand, within yobD at 1902.882 kb on + strand, within yobD at 1902.893 kb on + strand, within yobD at 1902.914 kb on + strand, within yobD at 1902.964 kb on + strand, within yobD at 1902.996 kb on + strand, within yobD at 1903.006 kb on - strand, within yobD at 1903.026 kb on - strand, within yobD at 1903.040 kb on + strand, within yobD at 1903.051 kb on + strand, within yobD at 1903.051 kb on + strand, within yobD at 1903.059 kb on - strand, within yobD at 1903.069 kb on - strand, within yobD at 1903.138 kb on + strand, within yobD at 1903.161 kb on + strand, within yobD at 1903.161 kb on + strand, within yobD at 1903.179 kb on - strand, within yobD at 1903.183 kb on + strand, within yobD at 1903.196 kb on + strand, within yobD at 1903.198 kb on + strand, within yobD at 1903.496 kb on - strand at 1903.498 kb on - strand at 1903.529 kb on + strand at 1903.537 kb on - strand at 1903.548 kb on + strand at 1903.553 kb on + strand at 1903.558 kb on + strand at 1903.558 kb on + strand at 1903.583 kb on + strand at 1903.602 kb on - strand at 1903.602 kb on - strand at 1903.639 kb on - strand at 1903.654 kb on - strand at 1903.702 kb on + strand at 1903.777 kb on - strand, within b1821 at 1903.777 kb on - strand, within b1821 at 1904.065 kb on - strand, within b1821 at 1904.087 kb on - strand, within b1821
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 1,902,131 + manZ b1819 0.25 -0.3 1,902,131 + manZ b1819 0.25 +0.3 1,902,139 - manZ b1819 0.26 +0.7 1,902,139 - manZ b1819 0.26 +0.5 1,902,146 + manZ b1819 0.27 +0.7 1,902,425 - manZ b1819 0.59 +0.7 1,902,428 + manZ b1819 0.60 -0.8 1,902,618 + manZ b1819 0.82 -0.7 1,902,618 + manZ b1819 0.82 -0.6 1,902,626 - manZ b1819 0.83 -1.3 1,902,758 + -0.4 1,902,763 + +0.3 1,902,765 + -0.1 1,902,765 - +0.3 1,902,766 - +0.3 1,902,773 - -2.7 1,902,781 - +1.3 1,902,867 + -0.3 1,902,867 + -1.9 1,902,875 - yobD b1820 0.11 -0.7 1,902,875 - -0.3 1,902,875 - yobD b1820 0.11 -3.1 1,902,882 + yobD b1820 0.12 -0.2 1,902,893 + yobD b1820 0.15 -1.3 1,902,914 + yobD b1820 0.19 -1.0 1,902,964 + yobD b1820 0.30 +0.7 1,902,996 + yobD b1820 0.37 -0.9 1,903,006 - yobD b1820 0.39 -0.4 1,903,026 - yobD b1820 0.44 -0.3 1,903,040 + yobD b1820 0.47 -0.9 1,903,051 + yobD b1820 0.49 -0.3 1,903,051 + yobD b1820 0.49 -0.5 1,903,059 - yobD b1820 0.51 +0.3 1,903,069 - yobD b1820 0.53 +0.7 1,903,138 + yobD b1820 0.68 -0.6 1,903,161 + yobD b1820 0.73 -1.9 1,903,161 + yobD b1820 0.73 +0.6 1,903,179 - yobD b1820 0.77 +1.3 1,903,183 + yobD b1820 0.78 +0.4 1,903,196 + yobD b1820 0.81 -1.0 1,903,198 + yobD b1820 0.81 +0.4 1,903,496 - -0.5 1,903,498 - +0.7 1,903,529 + -1.3 1,903,537 - -0.4 1,903,548 + -1.2 1,903,553 + +0.1 1,903,558 + +2.3 1,903,558 + +1.3 1,903,583 + -1.4 1,903,602 - -1.0 1,903,602 - -0.5 1,903,639 - +1.3 1,903,654 - -0.8 1,903,702 + +0.9 1,903,777 - b1821 b1821 0.11 -0.4 1,903,777 - b1821 b1821 0.11 +0.2 1,904,065 - b1821 b1821 0.62 +0.2 1,904,087 - b1821 b1821 0.66 +0.9
Or see this region's nucleotide sequence