Strain Fitness in Escherichia coli BW25113 around b0824

Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntybiW and ybiY are separated by 5 nucleotidesybiY and mipB are separated by 130 nucleotidesmipB and moeB are separated by 75 nucleotides b0823: ybiW - predicted pyruvate formate lyase (NCBI), at 859,397 to 861,829 ybiW b0824: ybiY - predicted pyruvate formate lyase activating enzyme (RefSeq), at 861,835 to 862,734 ybiY b0825: mipB - putative transaldolase (VIMSS), at 862,865 to 863,527 mipB b0826: moeB - molybdopterin biosynthesis protein MoeB (NCBI), at 863,603 to 864,352 moeB Position (kb) 861 862 863Strain fitness (log2 ratio) -1 0 1 2at 860.884 kb on - strand, within ybiWat 861.029 kb on - strand, within ybiWat 861.287 kb on - strand, within ybiWat 861.428 kb on + strand, within ybiWat 861.428 kb on + strand, within ybiWat 861.428 kb on + strand, within ybiWat 861.715 kb on + strandat 861.715 kb on + strandat 861.723 kb on - strandat 861.728 kb on - strandat 861.749 kb on - strandat 861.947 kb on - strand, within ybiYat 862.001 kb on + strand, within ybiYat 862.063 kb on - strand, within ybiYat 862.219 kb on - strand, within ybiYat 862.295 kb on + strand, within ybiYat 862.398 kb on - strand, within ybiYat 862.435 kb on + strand, within ybiYat 862.435 kb on + strand, within ybiYat 862.439 kb on - strand, within ybiYat 862.442 kb on + strand, within ybiYat 862.442 kb on + strand, within ybiYat 862.462 kb on + strand, within ybiYat 862.575 kb on + strand, within ybiYat 862.575 kb on + strand, within ybiYat 862.580 kb on + strand, within ybiYat 862.600 kb on + strand, within ybiYat 862.697 kb on - strandat 862.717 kb on - strandat 862.828 kb on + strandat 863.057 kb on + strand, within mipBat 863.251 kb on + strand, within mipBat 863.433 kb on + strand, within mipBat 863.554 kb on + strandat 863.625 kb on - strandat 863.673 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM
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860,884 - ybiW b0823 0.61 -0.5
861,029 - ybiW b0823 0.67 -0.8
861,287 - ybiW b0823 0.78 -0.5
861,428 + ybiW b0823 0.83 -0.3
861,428 + ybiW b0823 0.83 -1.3
861,428 + ybiW b0823 0.83 +0.5
861,715 + -0.7
861,715 + -0.2
861,723 - +0.0
861,728 - +0.5
861,749 - -1.6
861,947 - ybiY b0824 0.12 -1.3
862,001 + ybiY b0824 0.18 -0.6
862,063 - ybiY b0824 0.25 +0.4
862,219 - ybiY b0824 0.43 -1.0
862,295 + ybiY b0824 0.51 +0.1
862,398 - ybiY b0824 0.63 +1.5
862,435 + ybiY b0824 0.67 -0.4
862,435 + ybiY b0824 0.67 -0.3
862,439 - ybiY b0824 0.67 -0.6
862,442 + ybiY b0824 0.67 -0.2
862,442 + ybiY b0824 0.67 +0.3
862,462 + ybiY b0824 0.70 -1.1
862,575 + ybiY b0824 0.82 +0.2
862,575 + ybiY b0824 0.82 -0.6
862,580 + ybiY b0824 0.83 +0.5
862,600 + ybiY b0824 0.85 +1.8
862,697 - +0.5
862,717 - -0.2
862,828 + +1.5
863,057 + mipB b0825 0.29 +0.3
863,251 + mipB b0825 0.58 -0.6
863,433 + mipB b0825 0.86 -0.5
863,554 + -0.1
863,625 - -0.5
863,673 - +0.2

Or see this region's nucleotide sequence