Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cyoA and ampG are separated by 459 nucleotides ampG and yajG are separated by 43 nucleotides yajG and bolA are separated by 304 nucleotides
b0432: cyoA - cytochrome o ubiquinol oxidase subunit II (NCBI), at 449,887 to 450,834
cyoA
b0433: ampG - muropeptide transporter (NCBI), at 451,294 to 452,769
ampG
b0434: yajG - putative polymerase/proteinase (VIMSS), at 452,813 to 453,391
yajG
b0435: bolA - possible regulator of murein genes (VIMSS), at 453,696 to 454,013
bolA
Position (kb) 451
452
453 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 450.344 kb on + strand, within cyoA at 450.344 kb on + strand, within cyoA at 450.353 kb on + strand, within cyoA at 450.359 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.367 kb on - strand, within cyoA at 450.397 kb on + strand, within cyoA at 450.423 kb on - strand, within cyoA at 450.432 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.452 kb on - strand, within cyoA at 450.463 kb on + strand, within cyoA at 450.497 kb on - strand, within cyoA at 450.514 kb on + strand, within cyoA at 450.522 kb on + strand, within cyoA at 450.546 kb on - strand, within cyoA at 450.553 kb on + strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.562 kb on - strand, within cyoA at 450.575 kb on + strand, within cyoA at 450.581 kb on - strand, within cyoA at 450.597 kb on + strand, within cyoA at 450.601 kb on + strand, within cyoA at 450.601 kb on - strand, within cyoA at 450.673 kb on - strand, within cyoA at 450.687 kb on + strand, within cyoA at 450.702 kb on + strand, within cyoA at 450.707 kb on + strand, within cyoA at 450.710 kb on - strand, within cyoA at 450.715 kb on - strand, within cyoA at 450.718 kb on + strand, within cyoA at 450.725 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.730 kb on - strand, within cyoA at 450.753 kb on - strand at 450.763 kb on - strand at 450.769 kb on + strand at 450.769 kb on + strand at 450.774 kb on + strand at 450.777 kb on - strand at 450.779 kb on - strand at 450.779 kb on - strand at 450.782 kb on - strand at 450.782 kb on - strand at 450.792 kb on + strand at 450.863 kb on - strand at 450.863 kb on - strand at 450.874 kb on + strand at 450.874 kb on - strand at 450.893 kb on - strand at 450.904 kb on - strand at 450.904 kb on - strand at 450.950 kb on + strand at 450.954 kb on + strand at 450.954 kb on + strand at 450.957 kb on - strand at 450.973 kb on - strand at 450.996 kb on + strand at 451.004 kb on - strand at 451.021 kb on - strand at 451.023 kb on + strand at 451.026 kb on + strand at 451.041 kb on - strand at 451.041 kb on - strand at 451.044 kb on - strand at 451.213 kb on + strand at 451.277 kb on - strand at 451.325 kb on - strand at 451.328 kb on - strand at 451.328 kb on - strand at 451.347 kb on - strand at 451.386 kb on - strand at 451.386 kb on - strand at 451.394 kb on - strand at 451.402 kb on + strand at 451.403 kb on - strand at 451.449 kb on + strand, within ampG at 451.449 kb on + strand, within ampG at 451.466 kb on - strand, within ampG at 451.483 kb on - strand, within ampG at 451.590 kb on + strand, within ampG at 451.590 kb on + strand, within ampG at 451.596 kb on - strand, within ampG at 451.596 kb on - strand, within ampG at 451.596 kb on - strand, within ampG at 451.598 kb on - strand, within ampG at 451.630 kb on + strand, within ampG at 451.638 kb on - strand, within ampG at 451.683 kb on - strand, within ampG at 451.700 kb on - strand, within ampG at 451.700 kb on - strand, within ampG at 451.702 kb on - strand, within ampG at 451.742 kb on + strand, within ampG at 451.742 kb on + strand, within ampG at 451.742 kb on + strand, within ampG at 451.776 kb on + strand, within ampG at 451.776 kb on + strand, within ampG at 451.778 kb on - strand, within ampG at 451.809 kb on + strand, within ampG at 451.816 kb on + strand, within ampG at 451.816 kb on + strand, within ampG at 451.817 kb on + strand, within ampG at 451.822 kb on - strand, within ampG at 451.822 kb on - strand, within ampG at 451.843 kb on - strand, within ampG at 451.906 kb on - strand, within ampG at 451.906 kb on - strand, within ampG at 451.906 kb on - strand, within ampG at 451.915 kb on - strand, within ampG at 451.916 kb on - strand, within ampG at 451.916 kb on - strand, within ampG at 451.931 kb on + strand, within ampG at 451.988 kb on - strand, within ampG at 451.994 kb on - strand, within ampG at 451.994 kb on - strand, within ampG at 451.994 kb on - strand, within ampG at 451.995 kb on - strand, within ampG at 451.995 kb on - strand, within ampG at 452.055 kb on - strand, within ampG at 452.071 kb on - strand, within ampG at 452.081 kb on - strand, within ampG at 452.081 kb on - strand, within ampG at 452.099 kb on + strand, within ampG at 452.176 kb on + strand, within ampG at 452.184 kb on - strand, within ampG at 452.215 kb on + strand, within ampG at 452.221 kb on - strand at 452.221 kb on - strand, within ampG at 452.223 kb on - strand, within ampG at 452.237 kb on - strand, within ampG at 452.239 kb on + strand, within ampG at 452.239 kb on + strand, within ampG at 452.239 kb on + strand, within ampG at 452.247 kb on - strand, within ampG at 452.248 kb on + strand, within ampG at 452.248 kb on + strand, within ampG at 452.259 kb on + strand, within ampG at 452.271 kb on - strand, within ampG at 452.288 kb on - strand, within ampG at 452.288 kb on - strand, within ampG at 452.304 kb on + strand, within ampG at 452.304 kb on + strand, within ampG at 452.304 kb on + strand, within ampG at 452.337 kb on + strand, within ampG at 452.337 kb on + strand, within ampG at 452.345 kb on - strand, within ampG at 452.381 kb on + strand, within ampG at 452.388 kb on - strand, within ampG at 452.388 kb on - strand, within ampG at 452.390 kb on + strand, within ampG at 452.390 kb on + strand, within ampG at 452.403 kb on - strand, within ampG at 452.435 kb on - strand, within ampG at 452.435 kb on - strand, within ampG at 452.449 kb on + strand, within ampG at 452.449 kb on + strand, within ampG at 452.449 kb on - strand, within ampG at 452.449 kb on - strand, within ampG at 452.481 kb on + strand, within ampG at 452.552 kb on + strand, within ampG at 452.552 kb on + strand, within ampG at 452.568 kb on - strand, within ampG at 452.568 kb on - strand, within ampG at 452.573 kb on + strand, within ampG at 452.602 kb on + strand, within ampG at 452.602 kb on + strand, within ampG at 452.644 kb on + strand at 452.654 kb on + strand at 452.667 kb on + strand at 452.667 kb on - strand at 452.667 kb on - strand at 452.678 kb on - strand at 452.678 kb on - strand at 452.678 kb on - strand at 452.727 kb on - strand at 452.727 kb on - strand at 452.779 kb on - strand at 452.786 kb on + strand at 452.786 kb on + strand at 452.862 kb on - strand at 452.862 kb on - strand at 452.879 kb on + strand, within yajG at 452.879 kb on + strand, within yajG at 452.897 kb on - strand, within yajG at 452.897 kb on - strand, within yajG at 452.935 kb on + strand, within yajG at 452.935 kb on + strand, within yajG at 453.010 kb on - strand, within yajG at 453.010 kb on - strand, within yajG at 453.024 kb on + strand, within yajG at 453.024 kb on + strand, within yajG at 453.026 kb on + strand, within yajG at 453.026 kb on + strand, within yajG at 453.047 kb on + strand, within yajG at 453.047 kb on + strand, within yajG at 453.052 kb on + strand at 453.060 kb on - strand, within yajG at 453.065 kb on - strand, within yajG at 453.065 kb on - strand, within yajG at 453.138 kb on - strand, within yajG at 453.138 kb on - strand, within yajG at 453.198 kb on - strand, within yajG at 453.198 kb on - strand, within yajG at 453.221 kb on + strand, within yajG at 453.221 kb on + strand, within yajG at 453.228 kb on + strand, within yajG at 453.289 kb on - strand, within yajG at 453.289 kb on - strand, within yajG at 453.303 kb on + strand, within yajG at 453.339 kb on - strand at 453.478 kb on - strand at 453.486 kb on - strand at 453.486 kb on - strand at 453.538 kb on + strand at 453.647 kb on + strand at 453.668 kb on + strand at 453.668 kb on + strand at 453.703 kb on + strand at 453.703 kb on + strand at 453.705 kb on - strand at 453.705 kb on - strand at 453.708 kb on - strand at 453.765 kb on + strand, within bolA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 450,344 + cyoA b0432 0.48 +2.0 450,344 + cyoA b0432 0.48 +0.7 450,353 + cyoA b0432 0.49 +0.2 450,359 - cyoA b0432 0.50 -0.8 450,367 - cyoA b0432 0.51 -0.1 450,367 - cyoA b0432 0.51 +0.7 450,397 + cyoA b0432 0.54 -0.6 450,423 - cyoA b0432 0.57 -1.2 450,432 - cyoA b0432 0.57 -0.6 450,452 - cyoA b0432 0.60 -0.5 450,452 - cyoA b0432 0.60 -0.5 450,463 + cyoA b0432 0.61 +1.2 450,497 - cyoA b0432 0.64 -1.7 450,514 + cyoA b0432 0.66 -1.0 450,522 + cyoA b0432 0.67 -0.9 450,546 - cyoA b0432 0.70 +0.7 450,553 + cyoA b0432 0.70 -0.6 450,562 - cyoA b0432 0.71 +0.1 450,562 - cyoA b0432 0.71 -0.7 450,575 + cyoA b0432 0.73 -0.5 450,581 - cyoA b0432 0.73 -1.4 450,597 + cyoA b0432 0.75 +0.1 450,601 + cyoA b0432 0.75 -0.3 450,601 - cyoA b0432 0.75 -1.3 450,673 - cyoA b0432 0.83 +1.1 450,687 + cyoA b0432 0.84 +0.4 450,702 + cyoA b0432 0.86 -1.3 450,707 + cyoA b0432 0.86 +0.7 450,710 - cyoA b0432 0.87 +0.7 450,715 - cyoA b0432 0.87 -1.9 450,718 + cyoA b0432 0.88 +1.7 450,725 - cyoA b0432 0.88 -0.4 450,730 - cyoA b0432 0.89 +0.4 450,730 - cyoA b0432 0.89 +0.1 450,753 - +0.8 450,763 - -0.8 450,769 + +1.1 450,769 + -0.9 450,774 + -1.0 450,777 - -0.3 450,779 - -0.3 450,779 - -0.9 450,782 - -1.0 450,782 - -0.9 450,792 + +1.7 450,863 - +1.1 450,863 - -0.6 450,874 + -0.0 450,874 - +1.6 450,893 - -0.9 450,904 - -0.3 450,904 - +1.2 450,950 + +1.0 450,954 + -1.1 450,954 + +0.1 450,957 - -1.6 450,973 - +1.1 450,996 + -0.4 451,004 - +0.0 451,021 - +0.3 451,023 + +0.6 451,026 + -0.0 451,041 - -0.3 451,041 - +0.1 451,044 - -0.5 451,213 + -0.4 451,277 - +1.7 451,325 - -2.1 451,328 - -1.3 451,328 - +0.6 451,347 - -2.1 451,386 - -1.7 451,386 - -1.5 451,394 - -3.2 451,402 + +0.1 451,403 - +0.7 451,449 + ampG b0433 0.11 -3.0 451,449 + ampG b0433 0.11 -1.9 451,466 - ampG b0433 0.12 -1.2 451,483 - ampG b0433 0.13 -1.5 451,590 + ampG b0433 0.20 -0.3 451,590 + ampG b0433 0.20 -0.4 451,596 - ampG b0433 0.20 -4.1 451,596 - ampG b0433 0.20 -2.0 451,596 - ampG b0433 0.20 -2.2 451,598 - ampG b0433 0.21 -0.3 451,630 + ampG b0433 0.23 -2.9 451,638 - ampG b0433 0.23 -2.3 451,683 - ampG b0433 0.26 -0.9 451,700 - ampG b0433 0.28 -2.9 451,700 - ampG b0433 0.28 -1.7 451,702 - ampG b0433 0.28 -3.1 451,742 + ampG b0433 0.30 -3.2 451,742 + ampG b0433 0.30 -2.5 451,742 + ampG b0433 0.30 -3.2 451,776 + ampG b0433 0.33 -0.9 451,776 + ampG b0433 0.33 -3.3 451,778 - ampG b0433 0.33 -2.4 451,809 + ampG b0433 0.35 -2.1 451,816 + ampG b0433 0.35 -2.3 451,816 + ampG b0433 0.35 -1.6 451,817 + ampG b0433 0.35 -3.3 451,822 - ampG b0433 0.36 -1.0 451,822 - ampG b0433 0.36 +0.3 451,843 - ampG b0433 0.37 -1.9 451,906 - ampG b0433 0.41 -0.5 451,906 - ampG b0433 0.41 -1.6 451,906 - ampG b0433 0.41 -2.4 451,915 - ampG b0433 0.42 -0.5 451,916 - ampG b0433 0.42 -3.4 451,916 - ampG b0433 0.42 -3.4 451,931 + ampG b0433 0.43 -1.1 451,988 - ampG b0433 0.47 -2.1 451,994 - ampG b0433 0.47 -1.9 451,994 - ampG b0433 0.47 -2.0 451,994 - ampG b0433 0.47 -1.9 451,995 - ampG b0433 0.47 +0.1 451,995 - ampG b0433 0.47 -2.9 452,055 - ampG b0433 0.52 -1.9 452,071 - ampG b0433 0.53 -2.6 452,081 - ampG b0433 0.53 -2.4 452,081 - ampG b0433 0.53 -0.9 452,099 + ampG b0433 0.55 -0.3 452,176 + ampG b0433 0.60 -1.2 452,184 - ampG b0433 0.60 -1.7 452,215 + ampG b0433 0.62 -2.5 452,221 - +0.4 452,221 - ampG b0433 0.63 -2.4 452,223 - ampG b0433 0.63 -2.9 452,237 - ampG b0433 0.64 -0.3 452,239 + ampG b0433 0.64 -2.1 452,239 + ampG b0433 0.64 -1.1 452,239 + ampG b0433 0.64 -2.3 452,247 - ampG b0433 0.65 -1.5 452,248 + ampG b0433 0.65 -1.9 452,248 + ampG b0433 0.65 +0.4 452,259 + ampG b0433 0.65 -1.8 452,271 - ampG b0433 0.66 -2.0 452,288 - ampG b0433 0.67 -1.5 452,288 - ampG b0433 0.67 -1.6 452,304 + ampG b0433 0.68 +0.1 452,304 + ampG b0433 0.68 -1.1 452,304 + ampG b0433 0.68 -2.0 452,337 + ampG b0433 0.71 -2.9 452,337 + ampG b0433 0.71 -3.0 452,345 - ampG b0433 0.71 -1.5 452,381 + ampG b0433 0.74 -1.7 452,388 - ampG b0433 0.74 -0.3 452,388 - ampG b0433 0.74 -0.9 452,390 + ampG b0433 0.74 -3.0 452,390 + ampG b0433 0.74 -2.9 452,403 - ampG b0433 0.75 -1.9 452,435 - ampG b0433 0.77 +1.7 452,435 - ampG b0433 0.77 -1.9 452,449 + ampG b0433 0.78 -1.9 452,449 + ampG b0433 0.78 -0.6 452,449 - ampG b0433 0.78 -0.9 452,449 - ampG b0433 0.78 -0.6 452,481 + ampG b0433 0.80 -1.7 452,552 + ampG b0433 0.85 -3.1 452,552 + ampG b0433 0.85 -3.5 452,568 - ampG b0433 0.86 -1.6 452,568 - ampG b0433 0.86 -0.3 452,573 + ampG b0433 0.87 -0.5 452,602 + ampG b0433 0.89 -1.1 452,602 + ampG b0433 0.89 -3.0 452,644 + -4.4 452,654 + -2.2 452,667 + -1.5 452,667 - -0.9 452,667 - -0.3 452,678 - -1.3 452,678 - -4.5 452,678 - +1.7 452,727 - -0.7 452,727 - -2.1 452,779 - -1.6 452,786 + -2.9 452,786 + -1.9 452,862 - +0.5 452,862 - +0.4 452,879 + yajG b0434 0.11 -2.0 452,879 + yajG b0434 0.11 -2.2 452,897 - yajG b0434 0.15 -0.6 452,897 - yajG b0434 0.15 +0.6 452,935 + yajG b0434 0.21 -0.6 452,935 + yajG b0434 0.21 -1.7 453,010 - yajG b0434 0.34 -1.6 453,010 - yajG b0434 0.34 +0.5 453,024 + yajG b0434 0.36 -2.7 453,024 + yajG b0434 0.36 -3.1 453,026 + yajG b0434 0.37 -1.3 453,026 + yajG b0434 0.37 -1.6 453,047 + yajG b0434 0.40 -1.3 453,047 + yajG b0434 0.40 -3.2 453,052 + +1.7 453,060 - yajG b0434 0.43 -2.3 453,065 - yajG b0434 0.44 -1.9 453,065 - yajG b0434 0.44 +0.1 453,138 - yajG b0434 0.56 +0.5 453,138 - yajG b0434 0.56 -0.3 453,198 - yajG b0434 0.66 -2.2 453,198 - yajG b0434 0.66 -2.2 453,221 + yajG b0434 0.70 -2.2 453,221 + yajG b0434 0.70 -1.6 453,228 + yajG b0434 0.72 -2.7 453,289 - yajG b0434 0.82 -0.9 453,289 - yajG b0434 0.82 -2.7 453,303 + yajG b0434 0.85 -0.9 453,339 - +1.7 453,478 - -1.0 453,486 - +0.9 453,486 - -1.2 453,538 + +1.5 453,647 + +0.1 453,668 + +0.7 453,668 + +0.1 453,703 + +0.1 453,703 + -1.1 453,705 - +0.4 453,705 - -0.5 453,708 - -0.5 453,765 + bolA b0435 0.22 +0.8
Or see this region's nucleotide sequence