Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafV and ivy are separated by 153 nucleotides ivy and yafH are separated by 42 nucleotides yafH and lpcA are separated by 239 nucleotides
b0219: yafV - predicted C-N hydrolase family amidase, NAD(P)-binding (NCBI), at 239,419 to 240,189
yafV
b0220: ivy - inhibitor of vertebrate C-lysozyme (NCBI), at 240,343 to 240,816
ivy
b0221: yafH - putative acyl-CoA dehydrogenase (EC 1.3.99.-) (VIMSS), at 240,859 to 243,303
yafH
b0222: lpcA - phosphoheptose isomerase (NCBI), at 243,543 to 244,121
lpcA
Position (kb) 240
241
242
243
244 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4
5
6 at 239.907 kb on + strand, within yafV at 240.066 kb on + strand, within yafV at 240.072 kb on + strand, within yafV at 240.098 kb on - strand, within yafV at 240.145 kb on + strand at 240.148 kb on + strand at 240.175 kb on + strand at 240.197 kb on - strand at 240.197 kb on - strand at 240.199 kb on - strand at 240.199 kb on - strand at 240.224 kb on + strand at 240.261 kb on - strand at 240.325 kb on + strand at 240.325 kb on + strand at 240.333 kb on + strand at 240.350 kb on + strand at 240.350 kb on + strand at 240.358 kb on - strand at 240.406 kb on + strand, within ivy at 240.493 kb on + strand, within ivy at 240.521 kb on - strand, within ivy at 240.544 kb on + strand, within ivy at 240.544 kb on + strand, within ivy at 240.608 kb on + strand, within ivy at 240.717 kb on + strand, within ivy at 240.743 kb on - strand, within ivy at 240.743 kb on - strand, within ivy at 240.843 kb on - strand at 240.939 kb on + strand at 240.939 kb on + strand at 241.035 kb on + strand at 241.035 kb on + strand at 241.158 kb on + strand, within yafH at 241.164 kb on + strand, within yafH at 241.164 kb on + strand, within yafH at 241.168 kb on - strand, within yafH at 241.196 kb on + strand, within yafH at 241.256 kb on - strand, within yafH at 241.258 kb on - strand, within yafH at 241.347 kb on - strand, within yafH at 241.355 kb on - strand, within yafH at 241.355 kb on - strand, within yafH at 241.357 kb on - strand, within yafH at 241.374 kb on - strand, within yafH at 241.374 kb on - strand, within yafH at 241.387 kb on + strand, within yafH at 241.387 kb on + strand, within yafH at 241.397 kb on + strand, within yafH at 241.397 kb on + strand, within yafH at 241.408 kb on + strand, within yafH at 241.408 kb on + strand, within yafH at 241.426 kb on + strand, within yafH at 241.434 kb on + strand, within yafH at 241.453 kb on - strand, within yafH at 241.468 kb on + strand, within yafH at 241.520 kb on + strand, within yafH at 241.520 kb on + strand, within yafH at 241.622 kb on - strand, within yafH at 241.622 kb on - strand at 241.737 kb on + strand, within yafH at 241.837 kb on + strand, within yafH at 241.899 kb on + strand, within yafH at 242.069 kb on - strand, within yafH at 242.069 kb on - strand, within yafH at 242.161 kb on + strand, within yafH at 242.213 kb on + strand, within yafH at 242.257 kb on - strand, within yafH at 242.361 kb on + strand, within yafH at 242.361 kb on + strand, within yafH at 242.440 kb on - strand, within yafH at 242.460 kb on + strand, within yafH at 242.460 kb on + strand, within yafH at 242.599 kb on - strand, within yafH at 242.660 kb on + strand, within yafH at 242.660 kb on + strand, within yafH at 242.670 kb on - strand, within yafH at 242.822 kb on + strand, within yafH at 242.831 kb on - strand, within yafH at 242.831 kb on - strand, within yafH at 242.962 kb on + strand, within yafH at 243.011 kb on + strand, within yafH at 243.011 kb on + strand, within yafH at 243.019 kb on - strand, within yafH at 243.063 kb on + strand at 243.183 kb on - strand at 243.211 kb on - strand at 243.261 kb on + strand at 243.270 kb on + strand at 243.270 kb on + strand at 243.467 kb on + strand at 243.501 kb on - strand at 243.670 kb on - strand, within lpcA at 243.717 kb on + strand, within lpcA at 243.853 kb on + strand, within lpcA at 243.853 kb on + strand, within lpcA at 243.866 kb on - strand, within lpcA at 243.866 kb on - strand, within lpcA at 243.893 kb on - strand, within lpcA at 243.903 kb on + strand, within lpcA at 244.003 kb on + strand, within lpcA at 244.070 kb on - strand at 244.078 kb on + strand at 244.080 kb on - strand at 244.124 kb on - strand at 244.129 kb on - strand at 244.159 kb on + strand at 244.197 kb on + strand at 244.217 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 239,907 + yafV b0219 0.63 -2.5 240,066 + yafV b0219 0.84 +0.4 240,072 + yafV b0219 0.85 +0.1 240,098 - yafV b0219 0.88 -1.3 240,145 + +0.4 240,148 + -0.2 240,175 + -0.9 240,197 - -1.1 240,197 - -0.3 240,199 - +1.1 240,199 - +1.1 240,224 + +0.5 240,261 - +0.6 240,325 + -0.3 240,325 + -2.0 240,333 + +0.9 240,350 + -1.7 240,350 + -3.2 240,358 - +0.5 240,406 + ivy b0220 0.13 -0.4 240,493 + ivy b0220 0.32 +0.9 240,521 - ivy b0220 0.38 -0.1 240,544 + ivy b0220 0.42 -1.9 240,544 + ivy b0220 0.42 +1.5 240,608 + ivy b0220 0.56 -0.7 240,717 + ivy b0220 0.79 -0.7 240,743 - ivy b0220 0.84 +0.7 240,743 - ivy b0220 0.84 +0.1 240,843 - +0.1 240,939 + -1.2 240,939 + -1.5 241,035 + +0.7 241,035 + +0.6 241,158 + yafH b0221 0.12 +1.0 241,164 + yafH b0221 0.12 -2.6 241,164 + yafH b0221 0.12 -0.8 241,168 - yafH b0221 0.13 -0.2 241,196 + yafH b0221 0.14 +0.9 241,256 - yafH b0221 0.16 -1.3 241,258 - yafH b0221 0.16 +0.7 241,347 - yafH b0221 0.20 -0.9 241,355 - yafH b0221 0.20 -0.9 241,355 - yafH b0221 0.20 -0.7 241,357 - yafH b0221 0.20 +1.0 241,374 - yafH b0221 0.21 -0.5 241,374 - yafH b0221 0.21 -0.5 241,387 + yafH b0221 0.22 +0.1 241,387 + yafH b0221 0.22 -0.3 241,397 + yafH b0221 0.22 +0.1 241,397 + yafH b0221 0.22 +1.3 241,408 + yafH b0221 0.22 -0.7 241,408 + yafH b0221 0.22 -0.9 241,426 + yafH b0221 0.23 -0.9 241,434 + yafH b0221 0.24 -0.0 241,453 - yafH b0221 0.24 -0.1 241,468 + yafH b0221 0.25 +0.5 241,520 + yafH b0221 0.27 -0.2 241,520 + yafH b0221 0.27 +0.8 241,622 - yafH b0221 0.31 +0.9 241,622 - +1.7 241,737 + yafH b0221 0.36 +1.9 241,837 + yafH b0221 0.40 -0.1 241,899 + yafH b0221 0.43 -0.1 242,069 - yafH b0221 0.49 -1.8 242,069 - yafH b0221 0.49 -0.0 242,161 + yafH b0221 0.53 -2.0 242,213 + yafH b0221 0.55 +0.3 242,257 - yafH b0221 0.57 +0.3 242,361 + yafH b0221 0.61 -0.4 242,361 + yafH b0221 0.61 +1.0 242,440 - yafH b0221 0.65 -0.2 242,460 + yafH b0221 0.65 +0.8 242,460 + yafH b0221 0.65 +0.1 242,599 - yafH b0221 0.71 -0.3 242,660 + yafH b0221 0.74 +1.8 242,660 + yafH b0221 0.74 +0.6 242,670 - yafH b0221 0.74 -0.6 242,822 + yafH b0221 0.80 -0.4 242,831 - yafH b0221 0.81 -0.1 242,831 - yafH b0221 0.81 +0.8 242,962 + yafH b0221 0.86 -1.7 243,011 + yafH b0221 0.88 +1.7 243,011 + yafH b0221 0.88 +1.3 243,019 - yafH b0221 0.88 +0.9 243,063 + -0.5 243,183 - -1.4 243,211 - +0.1 243,261 + +0.3 243,270 + -0.7 243,270 + +0.5 243,467 + -0.2 243,501 - +0.2 243,670 - lpcA b0222 0.22 +2.5 243,717 + lpcA b0222 0.30 +2.0 243,853 + lpcA b0222 0.54 +2.4 243,853 + lpcA b0222 0.54 +6.2 243,866 - lpcA b0222 0.56 +4.8 243,866 - lpcA b0222 0.56 +3.8 243,893 - lpcA b0222 0.60 +4.0 243,903 + lpcA b0222 0.62 +2.9 244,003 + lpcA b0222 0.79 +3.9 244,070 - +4.9 244,078 + +2.0 244,080 - +5.2 244,124 - +3.9 244,129 - +1.4 244,159 + +0.5 244,197 + -0.2 244,217 - +0.9
Or see this region's nucleotide sequence