Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yafD and yafE are separated by 3 nucleotides yafE and mltD are separated by 47 nucleotides mltD and gloB are separated by 71 nucleotides gloB and yafS are separated by 33 nucleotides
b0209: yafD - hypothetical protein (NCBI), at 231,122 to 231,922
yafD
b0210: yafE - predicted S-adenosyl-L-methionine-dependent methyltransferase (NCBI), at 231,926 to 232,549
yafE
b0211: mltD - predicted membrane-bound lytic murein transglycosylase D (NCBI), at 232,597 to 233,955
mltD
b0212: gloB - predicted hydroxyacylglutathione hydrolase (NCBI), at 234,027 to 234,782
gloB
b0213: yafS - orf, hypothetical protein (VIMSS), at 234,816 to 235,538
yafS
Position (kb) 232
233
234 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 231.601 kb on + strand at 231.621 kb on + strand, within yafD at 231.627 kb on - strand, within yafD at 231.627 kb on - strand, within yafD at 231.659 kb on + strand, within yafD at 231.659 kb on + strand, within yafD at 231.664 kb on - strand, within yafD at 231.664 kb on - strand, within yafD at 231.667 kb on - strand, within yafD at 231.755 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.828 kb on + strand, within yafD at 231.837 kb on + strand, within yafD at 231.851 kb on + strand at 231.851 kb on + strand at 231.935 kb on - strand at 231.935 kb on - strand at 231.938 kb on + strand at 231.946 kb on - strand at 231.970 kb on + strand at 231.983 kb on + strand at 231.983 kb on + strand at 232.010 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.254 kb on + strand, within yafE at 232.303 kb on + strand, within yafE at 232.469 kb on + strand, within yafE at 232.656 kb on + strand at 232.678 kb on - strand at 232.728 kb on - strand at 232.728 kb on - strand at 232.760 kb on - strand, within mltD at 232.830 kb on + strand, within mltD at 232.830 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.839 kb on + strand, within mltD at 232.923 kb on - strand, within mltD at 232.923 kb on - strand, within mltD at 233.026 kb on - strand, within mltD at 233.047 kb on - strand, within mltD at 233.065 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.075 kb on - strand, within mltD at 233.156 kb on + strand, within mltD at 233.376 kb on - strand, within mltD at 233.376 kb on - strand, within mltD at 233.472 kb on + strand, within mltD at 233.472 kb on + strand, within mltD at 233.498 kb on - strand, within mltD at 233.697 kb on - strand, within mltD at 233.698 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.760 kb on - strand, within mltD at 233.774 kb on + strand, within mltD at 233.774 kb on + strand, within mltD at 233.875 kb on + strand at 233.914 kb on + strand at 234.054 kb on - strand at 234.253 kb on - strand, within gloB at 234.292 kb on + strand, within gloB at 234.292 kb on + strand, within gloB at 234.386 kb on + strand, within gloB at 234.386 kb on - strand, within gloB at 234.556 kb on - strand, within gloB at 234.737 kb on - strand at 234.737 kb on - strand at 234.796 kb on + strand at 234.796 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 231,601 + -0.3 231,621 + yafD b0209 0.62 -0.9 231,627 - yafD b0209 0.63 +0.8 231,627 - yafD b0209 0.63 +0.1 231,659 + yafD b0209 0.67 +0.3 231,659 + yafD b0209 0.67 +0.8 231,664 - yafD b0209 0.68 -1.0 231,664 - yafD b0209 0.68 +0.3 231,667 - yafD b0209 0.68 -0.9 231,755 + yafD b0209 0.79 -0.4 231,828 + yafD b0209 0.88 +1.7 231,828 + yafD b0209 0.88 -0.3 231,837 + yafD b0209 0.89 -3.0 231,851 + +0.3 231,851 + -0.1 231,935 - +0.2 231,935 - -0.3 231,938 + +0.1 231,946 - -1.5 231,970 + -0.9 231,983 + -0.1 231,983 + +0.1 232,010 + yafE b0210 0.13 +0.4 232,254 + yafE b0210 0.53 -0.6 232,254 + yafE b0210 0.53 +0.4 232,254 + yafE b0210 0.53 +1.7 232,303 + yafE b0210 0.60 -0.8 232,469 + yafE b0210 0.87 -2.2 232,656 + +0.2 232,678 - -0.4 232,728 - +0.3 232,728 - +0.7 232,760 - mltD b0211 0.12 +1.1 232,830 + mltD b0211 0.17 +1.7 232,830 + mltD b0211 0.17 +1.0 232,839 + mltD b0211 0.18 +0.0 232,839 + mltD b0211 0.18 -0.3 232,923 - mltD b0211 0.24 -0.7 232,923 - mltD b0211 0.24 +0.1 233,026 - mltD b0211 0.32 +0.5 233,047 - mltD b0211 0.33 -0.2 233,065 - mltD b0211 0.34 +0.9 233,075 - mltD b0211 0.35 -0.8 233,075 - mltD b0211 0.35 -0.9 233,156 + mltD b0211 0.41 +0.6 233,376 - mltD b0211 0.57 +0.1 233,376 - mltD b0211 0.57 -0.7 233,472 + mltD b0211 0.64 -1.3 233,472 + mltD b0211 0.64 -0.3 233,498 - mltD b0211 0.66 -0.9 233,697 - mltD b0211 0.81 +0.5 233,698 - mltD b0211 0.81 -2.0 233,760 - mltD b0211 0.86 +0.8 233,760 - mltD b0211 0.86 +0.0 233,774 + mltD b0211 0.87 +2.5 233,774 + mltD b0211 0.87 +0.9 233,875 + +2.3 233,914 + -0.1 234,054 - -0.5 234,253 - gloB b0212 0.30 +0.3 234,292 + gloB b0212 0.35 -0.1 234,292 + gloB b0212 0.35 -0.7 234,386 + gloB b0212 0.47 -0.3 234,386 - gloB b0212 0.47 +0.1 234,556 - gloB b0212 0.70 +0.6 234,737 - +2.4 234,737 - +0.1 234,796 + +1.3 234,796 + +0.3
Or see this region's nucleotide sequence