Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fhuA and fhuC are separated by 50 nucleotides fhuC and fhuD overlap by 1 nucleotides fhuD and fhuB overlap by 4 nucleotides
b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727
fhuA
b0151: fhuC - iron-hydroxamate transporter subunit (NCBI), at 169,778 to 170,575
fhuC
b0152: fhuD - iron-hydroxamate transporter subunit (NCBI), at 170,575 to 171,465
fhuD
b0153: fhuB - fused iron-hydroxamate transporter subunits of ABC superfamily: membrane components (NCBI), at 171,462 to 173,444
fhuB
Position (kb) 170
171
172 Strain fitness (log2 ratio) -2
-1
0
1
2 at 169.594 kb on - strand at 169.620 kb on + strand at 169.629 kb on - strand at 169.635 kb on - strand at 169.635 kb on - strand at 169.639 kb on - strand at 169.684 kb on - strand at 169.827 kb on - strand at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.926 kb on + strand, within fhuC at 169.929 kb on - strand, within fhuC at 169.965 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.966 kb on + strand, within fhuC at 169.989 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.210 kb on + strand, within fhuC at 170.250 kb on + strand, within fhuC at 170.250 kb on - strand, within fhuC at 170.255 kb on + strand, within fhuC at 170.257 kb on + strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.390 kb on - strand, within fhuC at 170.395 kb on - strand, within fhuC at 170.654 kb on - strand at 170.671 kb on + strand, within fhuD at 170.671 kb on + strand, within fhuD at 170.689 kb on - strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.739 kb on + strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.755 kb on - strand, within fhuD at 170.775 kb on + strand, within fhuD at 170.831 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.869 kb on + strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.895 kb on - strand, within fhuD at 170.972 kb on - strand, within fhuD at 171.065 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.199 kb on + strand, within fhuD at 171.219 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.225 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.228 kb on + strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.238 kb on - strand, within fhuD at 171.307 kb on - strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.336 kb on + strand, within fhuD at 171.423 kb on + strand at 171.423 kb on - strand at 171.423 kb on - strand at 171.506 kb on + strand at 171.514 kb on - strand at 171.533 kb on - strand at 171.678 kb on + strand, within fhuB at 171.678 kb on + strand, within fhuB at 171.680 kb on + strand, within fhuB at 171.680 kb on + strand, within fhuB at 171.680 kb on - strand, within fhuB at 171.680 kb on - strand, within fhuB at 171.695 kb on + strand, within fhuB at 171.742 kb on + strand, within fhuB at 171.794 kb on + strand, within fhuB at 171.867 kb on + strand, within fhuB at 171.910 kb on + strand, within fhuB at 171.910 kb on + strand, within fhuB at 172.063 kb on + strand, within fhuB at 172.088 kb on - strand, within fhuB at 172.202 kb on + strand, within fhuB at 172.218 kb on - strand, within fhuB at 172.222 kb on - strand, within fhuB at 172.261 kb on + strand, within fhuB at 172.281 kb on + strand, within fhuB at 172.281 kb on + strand, within fhuB at 172.289 kb on + strand, within fhuB at 172.290 kb on - strand, within fhuB at 172.293 kb on + strand, within fhuB at 172.293 kb on + strand, within fhuB at 172.314 kb on + strand, within fhuB at 172.369 kb on - strand, within fhuB
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 169,594 - +1.6 169,620 + -0.4 169,629 - -1.8 169,635 - +1.1 169,635 - -1.5 169,639 - +0.1 169,684 - +0.4 169,827 - -2.4 169,926 + fhuC b0151 0.19 +0.3 169,926 + fhuC b0151 0.19 +0.1 169,926 + fhuC b0151 0.19 -0.3 169,929 - fhuC b0151 0.19 -0.6 169,965 + fhuC b0151 0.23 -0.8 169,966 + fhuC b0151 0.24 +1.6 169,966 + fhuC b0151 0.24 -0.4 169,989 + fhuC b0151 0.26 +1.0 170,210 + fhuC b0151 0.54 +0.5 170,210 + fhuC b0151 0.54 -1.6 170,250 + fhuC b0151 0.59 -1.0 170,250 - fhuC b0151 0.59 -0.4 170,255 + fhuC b0151 0.60 +1.9 170,257 + fhuC b0151 0.60 +0.4 170,390 - fhuC b0151 0.77 -1.4 170,390 - fhuC b0151 0.77 -0.2 170,395 - fhuC b0151 0.77 -0.4 170,654 - -0.5 170,671 + fhuD b0152 0.11 -0.8 170,671 + fhuD b0152 0.11 -0.5 170,689 - fhuD b0152 0.13 -0.1 170,739 + fhuD b0152 0.18 -1.9 170,739 + fhuD b0152 0.18 -0.4 170,755 - fhuD b0152 0.20 +0.6 170,755 - fhuD b0152 0.20 +0.4 170,775 + fhuD b0152 0.22 -1.9 170,831 + fhuD b0152 0.29 -0.3 170,869 + fhuD b0152 0.33 -0.9 170,869 + fhuD b0152 0.33 -0.0 170,895 - fhuD b0152 0.36 -1.9 170,895 - fhuD b0152 0.36 +2.7 170,972 - fhuD b0152 0.45 +1.3 171,065 + fhuD b0152 0.55 +0.4 171,199 + fhuD b0152 0.70 +2.2 171,199 + fhuD b0152 0.70 -2.4 171,219 + fhuD b0152 0.72 -0.4 171,225 + fhuD b0152 0.73 -0.2 171,225 + fhuD b0152 0.73 +0.3 171,228 + fhuD b0152 0.73 -2.5 171,228 + fhuD b0152 0.73 -0.9 171,238 - fhuD b0152 0.74 -2.1 171,238 - fhuD b0152 0.74 -1.8 171,307 - fhuD b0152 0.82 -0.7 171,336 + fhuD b0152 0.85 -0.7 171,336 + fhuD b0152 0.85 -2.4 171,423 + -0.1 171,423 - -1.4 171,423 - -0.4 171,506 + -0.5 171,514 - +0.6 171,533 - -0.4 171,678 + fhuB b0153 0.11 +0.1 171,678 + fhuB b0153 0.11 -0.4 171,680 + fhuB b0153 0.11 -0.8 171,680 + fhuB b0153 0.11 -1.2 171,680 - fhuB b0153 0.11 -0.0 171,680 - fhuB b0153 0.11 +0.2 171,695 + fhuB b0153 0.12 -0.4 171,742 + fhuB b0153 0.14 -0.5 171,794 + fhuB b0153 0.17 -0.9 171,867 + fhuB b0153 0.20 -1.7 171,910 + fhuB b0153 0.23 -2.1 171,910 + fhuB b0153 0.23 -0.6 172,063 + fhuB b0153 0.30 +0.5 172,088 - fhuB b0153 0.32 +0.6 172,202 + fhuB b0153 0.37 -0.2 172,218 - fhuB b0153 0.38 +0.8 172,222 - fhuB b0153 0.38 -1.0 172,261 + fhuB b0153 0.40 +0.1 172,281 + fhuB b0153 0.41 -0.4 172,281 + fhuB b0153 0.41 -2.3 172,289 + fhuB b0153 0.42 +0.6 172,290 - fhuB b0153 0.42 +0.1 172,293 + fhuB b0153 0.42 -1.8 172,293 + fhuB b0153 0.42 +1.1 172,314 + fhuB b0153 0.43 +0.0 172,369 - fhuB b0153 0.46 -0.8
Or see this region's nucleotide sequence