Experiment: LB Anaerobic with Amoxicillin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hrpB and mrcB are separated by 195 nucleotides mrcB and fhuA are separated by 219 nucleotides
b0148: hrpB - helicase, ATP-dependent (VIMSS), at 162,105 to 164,534
hrpB
b0149: mrcB - penicillin-binding protein 1b (NCBI), at 164,730 to 167,264
mrcB
b0150: fhuA - ferrichrome outer membrane transporter (NCBI), at 167,484 to 169,727
fhuA
Position (kb) 164
165
166
167
168 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3 at 163.776 kb on - strand, within hrpB at 163.782 kb on + strand, within hrpB at 163.811 kb on + strand, within hrpB at 163.811 kb on - strand, within hrpB at 163.811 kb on - strand, within hrpB at 163.819 kb on - strand, within hrpB at 163.819 kb on - strand, within hrpB at 163.834 kb on - strand, within hrpB at 163.904 kb on + strand, within hrpB at 163.904 kb on + strand, within hrpB at 163.938 kb on + strand, within hrpB at 163.938 kb on + strand, within hrpB at 164.040 kb on + strand, within hrpB at 164.040 kb on + strand, within hrpB at 164.114 kb on - strand, within hrpB at 164.115 kb on + strand, within hrpB at 164.174 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.219 kb on + strand, within hrpB at 164.227 kb on - strand, within hrpB at 164.237 kb on - strand, within hrpB at 164.237 kb on - strand, within hrpB at 164.364 kb on - strand at 164.366 kb on + strand at 164.374 kb on - strand at 164.374 kb on - strand at 164.422 kb on - strand at 164.496 kb on - strand at 164.525 kb on + strand at 164.525 kb on + strand at 164.553 kb on + strand at 164.568 kb on - strand at 164.568 kb on - strand at 164.604 kb on + strand at 164.606 kb on - strand at 164.612 kb on - strand at 164.612 kb on - strand at 164.625 kb on - strand at 164.649 kb on - strand at 164.712 kb on + strand at 164.728 kb on - strand at 164.737 kb on + strand at 164.788 kb on + strand at 164.844 kb on + strand at 164.844 kb on + strand at 164.846 kb on + strand at 165.082 kb on - strand, within mrcB at 165.089 kb on - strand, within mrcB at 165.127 kb on + strand, within mrcB at 165.181 kb on + strand, within mrcB at 165.228 kb on - strand, within mrcB at 165.305 kb on + strand, within mrcB at 165.311 kb on + strand, within mrcB at 165.363 kb on - strand, within mrcB at 165.419 kb on + strand, within mrcB at 165.468 kb on - strand, within mrcB at 165.475 kb on + strand, within mrcB at 165.513 kb on + strand, within mrcB at 165.519 kb on - strand, within mrcB at 165.521 kb on - strand, within mrcB at 165.599 kb on + strand, within mrcB at 165.609 kb on + strand, within mrcB at 165.622 kb on - strand, within mrcB at 165.643 kb on + strand, within mrcB at 165.656 kb on + strand, within mrcB at 165.656 kb on + strand, within mrcB at 165.656 kb on + strand, within mrcB at 165.656 kb on + strand, within mrcB at 165.664 kb on - strand, within mrcB at 165.664 kb on - strand, within mrcB at 165.664 kb on - strand, within mrcB at 165.677 kb on - strand, within mrcB at 165.733 kb on - strand, within mrcB at 165.742 kb on + strand, within mrcB at 165.752 kb on - strand, within mrcB at 165.763 kb on + strand, within mrcB at 165.796 kb on - strand, within mrcB at 165.811 kb on - strand, within mrcB at 165.811 kb on - strand, within mrcB at 165.845 kb on + strand, within mrcB at 165.845 kb on + strand, within mrcB at 165.845 kb on + strand, within mrcB at 165.853 kb on - strand, within mrcB at 165.853 kb on - strand, within mrcB at 165.878 kb on + strand, within mrcB at 165.881 kb on - strand, within mrcB at 165.881 kb on - strand, within mrcB at 165.883 kb on - strand, within mrcB at 165.883 kb on - strand, within mrcB at 165.891 kb on + strand, within mrcB at 165.899 kb on + strand, within mrcB at 165.905 kb on + strand, within mrcB at 165.909 kb on - strand, within mrcB at 165.955 kb on + strand, within mrcB at 165.955 kb on + strand, within mrcB at 165.955 kb on + strand, within mrcB at 166.041 kb on - strand, within mrcB at 166.060 kb on - strand, within mrcB at 166.060 kb on - strand, within mrcB at 166.065 kb on + strand, within mrcB at 166.065 kb on + strand, within mrcB at 166.065 kb on + strand, within mrcB at 166.100 kb on + strand, within mrcB at 166.119 kb on - strand, within mrcB at 166.119 kb on - strand, within mrcB at 166.166 kb on + strand, within mrcB at 166.172 kb on - strand, within mrcB at 166.203 kb on - strand, within mrcB at 166.218 kb on + strand, within mrcB at 166.218 kb on + strand, within mrcB at 166.218 kb on + strand, within mrcB at 166.218 kb on + strand, within mrcB at 166.218 kb on + strand, within mrcB at 166.226 kb on - strand, within mrcB at 166.226 kb on - strand, within mrcB at 166.226 kb on - strand, within mrcB at 166.226 kb on - strand, within mrcB at 166.246 kb on + strand, within mrcB at 166.246 kb on + strand, within mrcB at 166.316 kb on + strand, within mrcB at 166.316 kb on + strand, within mrcB at 166.319 kb on - strand, within mrcB at 166.333 kb on - strand, within mrcB at 166.333 kb on - strand, within mrcB at 166.377 kb on + strand, within mrcB at 166.377 kb on + strand, within mrcB at 166.434 kb on - strand, within mrcB at 166.442 kb on + strand, within mrcB at 166.442 kb on + strand, within mrcB at 166.481 kb on + strand, within mrcB at 166.520 kb on - strand, within mrcB at 166.621 kb on + strand, within mrcB at 166.621 kb on + strand, within mrcB at 166.627 kb on + strand, within mrcB at 166.627 kb on + strand, within mrcB at 166.635 kb on - strand, within mrcB at 166.672 kb on + strand, within mrcB at 166.682 kb on + strand, within mrcB at 166.682 kb on + strand, within mrcB at 166.713 kb on + strand, within mrcB at 166.732 kb on + strand, within mrcB at 166.756 kb on + strand, within mrcB at 166.756 kb on + strand, within mrcB at 166.757 kb on - strand, within mrcB at 166.774 kb on - strand, within mrcB at 166.839 kb on - strand, within mrcB at 166.848 kb on + strand, within mrcB at 166.858 kb on - strand, within mrcB at 166.898 kb on - strand, within mrcB at 166.932 kb on + strand, within mrcB at 166.934 kb on - strand, within mrcB at 166.961 kb on + strand, within mrcB at 167.081 kb on + strand at 167.095 kb on - strand at 167.103 kb on - strand at 167.103 kb on - strand at 167.124 kb on + strand at 167.127 kb on - strand at 167.127 kb on - strand at 167.135 kb on - strand at 167.135 kb on - strand at 167.210 kb on + strand at 167.210 kb on + strand at 167.214 kb on + strand at 167.214 kb on + strand at 167.232 kb on - strand at 167.244 kb on - strand at 167.244 kb on - strand at 167.258 kb on + strand at 167.463 kb on + strand at 167.546 kb on - strand at 167.568 kb on + strand at 167.626 kb on - strand at 167.626 kb on - strand at 167.734 kb on - strand, within fhuA at 167.831 kb on + strand, within fhuA at 167.831 kb on + strand, within fhuA at 167.838 kb on + strand, within fhuA at 167.838 kb on + strand, within fhuA at 167.863 kb on + strand, within fhuA at 167.863 kb on + strand, within fhuA at 167.955 kb on + strand, within fhuA at 167.963 kb on - strand, within fhuA at 168.024 kb on + strand, within fhuA at 168.024 kb on + strand, within fhuA at 168.026 kb on + strand, within fhuA at 168.034 kb on - strand, within fhuA at 168.072 kb on + strand, within fhuA at 168.072 kb on + strand, within fhuA at 168.091 kb on + strand, within fhuA at 168.138 kb on - strand, within fhuA at 168.138 kb on - strand, within fhuA at 168.177 kb on - strand, within fhuA at 168.191 kb on - strand, within fhuA at 168.191 kb on - strand, within fhuA
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Amoxicillin 0.0000078125 mM remove 163,776 - hrpB b0148 0.69 -1.7 163,782 + hrpB b0148 0.69 +2.8 163,811 + hrpB b0148 0.70 +2.4 163,811 - hrpB b0148 0.70 -0.4 163,811 - hrpB b0148 0.70 +2.2 163,819 - hrpB b0148 0.71 +0.5 163,819 - hrpB b0148 0.71 +1.6 163,834 - hrpB b0148 0.71 +0.0 163,904 + hrpB b0148 0.74 +2.8 163,904 + hrpB b0148 0.74 +3.2 163,938 + hrpB b0148 0.75 +2.6 163,938 + hrpB b0148 0.75 +2.3 164,040 + hrpB b0148 0.80 +2.9 164,040 + hrpB b0148 0.80 +2.9 164,114 - hrpB b0148 0.83 +1.4 164,115 + hrpB b0148 0.83 +3.0 164,174 + hrpB b0148 0.85 +2.9 164,219 + hrpB b0148 0.87 +2.2 164,219 + hrpB b0148 0.87 +3.0 164,219 + hrpB b0148 0.87 +3.1 164,219 + hrpB b0148 0.87 +3.4 164,227 - hrpB b0148 0.87 -1.1 164,237 - hrpB b0148 0.88 +0.6 164,237 - hrpB b0148 0.88 -1.4 164,364 - +0.2 164,366 + +3.4 164,374 - -1.5 164,374 - -0.9 164,422 - -0.7 164,496 - -1.5 164,525 + +2.7 164,525 + +1.5 164,553 + +3.1 164,568 - +0.5 164,568 - -0.9 164,604 + +3.1 164,606 - -0.4 164,612 - +0.8 164,612 - +1.0 164,625 - -0.4 164,649 - -1.1 164,712 + +3.5 164,728 - +1.6 164,737 + +1.1 164,788 + +1.6 164,844 + -2.5 164,844 + -2.5 164,846 + +1.1 165,082 - mrcB b0149 0.14 -1.5 165,089 - mrcB b0149 0.14 -2.2 165,127 + mrcB b0149 0.16 -2.1 165,181 + mrcB b0149 0.18 -2.5 165,228 - mrcB b0149 0.20 -2.2 165,305 + mrcB b0149 0.23 -1.5 165,311 + mrcB b0149 0.23 -1.4 165,363 - mrcB b0149 0.25 -0.9 165,419 + mrcB b0149 0.27 -1.7 165,468 - mrcB b0149 0.29 -1.8 165,475 + mrcB b0149 0.29 -1.2 165,513 + mrcB b0149 0.31 -0.4 165,519 - mrcB b0149 0.31 -2.8 165,521 - mrcB b0149 0.31 -2.3 165,599 + mrcB b0149 0.34 -2.3 165,609 + mrcB b0149 0.35 -2.3 165,622 - mrcB b0149 0.35 -1.9 165,643 + mrcB b0149 0.36 -1.7 165,656 + mrcB b0149 0.37 +0.1 165,656 + mrcB b0149 0.37 -1.2 165,656 + mrcB b0149 0.37 -1.4 165,656 + mrcB b0149 0.37 +0.6 165,664 - mrcB b0149 0.37 -0.7 165,664 - mrcB b0149 0.37 -3.5 165,664 - mrcB b0149 0.37 -2.9 165,677 - mrcB b0149 0.37 -0.7 165,733 - mrcB b0149 0.40 -2.4 165,742 + mrcB b0149 0.40 -1.8 165,752 - mrcB b0149 0.40 -2.4 165,763 + mrcB b0149 0.41 -2.5 165,796 - mrcB b0149 0.42 -0.9 165,811 - mrcB b0149 0.43 +0.6 165,811 - mrcB b0149 0.43 -0.4 165,845 + mrcB b0149 0.44 -0.7 165,845 + mrcB b0149 0.44 +0.1 165,845 + mrcB b0149 0.44 +0.6 165,853 - mrcB b0149 0.44 -0.9 165,853 - mrcB b0149 0.44 -0.4 165,878 + mrcB b0149 0.45 -3.3 165,881 - mrcB b0149 0.45 +0.6 165,881 - mrcB b0149 0.45 -1.5 165,883 - mrcB b0149 0.45 +0.6 165,883 - mrcB b0149 0.45 -0.7 165,891 + mrcB b0149 0.46 -2.4 165,899 + mrcB b0149 0.46 -1.9 165,905 + mrcB b0149 0.46 -0.9 165,909 - mrcB b0149 0.47 -1.2 165,955 + mrcB b0149 0.48 -2.3 165,955 + mrcB b0149 0.48 -1.4 165,955 + mrcB b0149 0.48 +0.1 166,041 - mrcB b0149 0.52 -2.6 166,060 - mrcB b0149 0.52 -0.7 166,060 - mrcB b0149 0.52 -2.3 166,065 + mrcB b0149 0.53 -1.4 166,065 + mrcB b0149 0.53 -0.4 166,065 + mrcB b0149 0.53 -0.9 166,100 + mrcB b0149 0.54 -0.7 166,119 - mrcB b0149 0.55 -1.5 166,119 - mrcB b0149 0.55 +0.6 166,166 + mrcB b0149 0.57 -2.8 166,172 - mrcB b0149 0.57 -1.5 166,203 - mrcB b0149 0.58 +0.1 166,218 + mrcB b0149 0.59 -1.7 166,218 + mrcB b0149 0.59 -2.8 166,218 + mrcB b0149 0.59 -0.7 166,218 + mrcB b0149 0.59 -1.7 166,218 + mrcB b0149 0.59 -1.9 166,226 - mrcB b0149 0.59 -0.7 166,226 - mrcB b0149 0.59 -1.8 166,226 - mrcB b0149 0.59 +0.6 166,226 - mrcB b0149 0.59 +0.1 166,246 + mrcB b0149 0.60 -2.4 166,246 + mrcB b0149 0.60 -1.8 166,316 + mrcB b0149 0.63 +0.6 166,316 + mrcB b0149 0.63 -1.4 166,319 - mrcB b0149 0.63 -1.8 166,333 - mrcB b0149 0.63 -1.8 166,333 - mrcB b0149 0.63 +0.6 166,377 + mrcB b0149 0.65 -0.9 166,377 + mrcB b0149 0.65 -1.2 166,434 - mrcB b0149 0.67 -1.4 166,442 + mrcB b0149 0.68 -1.2 166,442 + mrcB b0149 0.68 -1.8 166,481 + mrcB b0149 0.69 -3.4 166,520 - mrcB b0149 0.71 +0.1 166,621 + mrcB b0149 0.75 +0.1 166,621 + mrcB b0149 0.75 -1.2 166,627 + mrcB b0149 0.75 -1.7 166,627 + mrcB b0149 0.75 -0.7 166,635 - mrcB b0149 0.75 -1.7 166,672 + mrcB b0149 0.77 -1.8 166,682 + mrcB b0149 0.77 +0.6 166,682 + mrcB b0149 0.77 -1.4 166,713 + mrcB b0149 0.78 -1.5 166,732 + mrcB b0149 0.79 -2.4 166,756 + mrcB b0149 0.80 -1.8 166,756 + mrcB b0149 0.80 -1.7 166,757 - mrcB b0149 0.80 -1.4 166,774 - mrcB b0149 0.81 -1.4 166,839 - mrcB b0149 0.83 -1.5 166,848 + mrcB b0149 0.84 -1.7 166,858 - mrcB b0149 0.84 -2.6 166,898 - mrcB b0149 0.86 -2.2 166,932 + mrcB b0149 0.87 -2.2 166,934 - mrcB b0149 0.87 -3.4 166,961 + mrcB b0149 0.88 -2.6 167,081 + -2.7 167,095 - -2.9 167,103 - +0.1 167,103 - +0.1 167,124 + -3.6 167,127 - -2.1 167,127 - -3.2 167,135 - -1.8 167,135 - -0.7 167,210 + -2.2 167,210 + -2.0 167,214 + -4.2 167,214 + -2.8 167,232 - -2.9 167,244 - -0.2 167,244 - -2.1 167,258 + +0.2 167,463 + -0.8 167,546 - -0.7 167,568 + -0.3 167,626 - +0.6 167,626 - +0.6 167,734 - fhuA b0150 0.11 +0.2 167,831 + fhuA b0150 0.15 -0.0 167,831 + fhuA b0150 0.15 -0.9 167,838 + fhuA b0150 0.16 -0.6 167,838 + fhuA b0150 0.16 -1.2 167,863 + fhuA b0150 0.17 -1.6 167,863 + fhuA b0150 0.17 +2.6 167,955 + fhuA b0150 0.21 -1.0 167,963 - fhuA b0150 0.21 -1.2 168,024 + fhuA b0150 0.24 +0.3 168,024 + fhuA b0150 0.24 +0.1 168,026 + fhuA b0150 0.24 +0.4 168,034 - fhuA b0150 0.25 -0.9 168,072 + fhuA b0150 0.26 -0.8 168,072 + fhuA b0150 0.26 +0.7 168,091 + fhuA b0150 0.27 -0.2 168,138 - fhuA b0150 0.29 +0.1 168,138 - fhuA b0150 0.29 -0.4 168,177 - fhuA b0150 0.31 -1.0 168,191 - fhuA b0150 0.32 -1.1 168,191 - fhuA b0150 0.32 +0.3
Or see this region's nucleotide sequence