Strain Fitness in Escherichia coli BW25113 around b2010

Experiment: LB Anaerobic with Ticarcillin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyeeA and sbmC are separated by 197 nucleotidessbmC and dacD are separated by 118 nucleotidesdacD and sbcB are separated by 208 nucleotides b2008: yeeA - conserved inner membrane protein (NCBI), at 2,077,557 to 2,078,615 yeeA b2009: sbmC - DNA gyrase inhibitor (NCBI), at 2,078,813 to 2,079,286 sbmC b2010: dacD - penicillin binding protein 6b (VIMSS), at 2,079,405 to 2,080,571 dacD b2011: sbcB - exonuclease I (NCBI), at 2,080,780 to 2,082,207 sbcB Position (kb) 2079 2080 2081Strain fitness (log2 ratio) -2 -1 0 1at 2078.611 kb on - strandat 2078.684 kb on - strandat 2078.689 kb on - strandat 2078.853 kb on + strandat 2078.861 kb on - strand, within sbmCat 2078.864 kb on + strand, within sbmCat 2079.000 kb on + strand, within sbmCat 2079.077 kb on + strand, within sbmCat 2079.281 kb on + strandat 2079.472 kb on - strandat 2079.472 kb on - strandat 2079.505 kb on - strandat 2079.568 kb on - strand, within dacDat 2079.612 kb on + strand, within dacDat 2079.630 kb on + strand, within dacDat 2079.784 kb on + strand, within dacDat 2079.833 kb on - strand, within dacDat 2079.833 kb on - strand, within dacDat 2079.857 kb on + strand, within dacDat 2079.857 kb on + strand, within dacDat 2079.966 kb on + strand, within dacDat 2080.001 kb on + strand, within dacDat 2080.041 kb on + strand, within dacDat 2080.420 kb on + strand, within dacDat 2080.420 kb on + strand, within dacDat 2080.685 kb on - strandat 2080.685 kb on - strandat 2080.691 kb on - strandat 2080.744 kb on + strandat 2080.777 kb on - strandat 2080.787 kb on - strandat 2080.857 kb on - strandat 2080.857 kb on - strandat 2080.867 kb on - strandat 2080.867 kb on - strandat 2080.982 kb on + strand, within sbcBat 2080.982 kb on + strand, within sbcBat 2080.990 kb on - strand, within sbcBat 2081.062 kb on + strand, within sbcBat 2081.062 kb on + strand, within sbcBat 2081.077 kb on - strand, within sbcBat 2081.151 kb on + strand, within sbcBat 2081.153 kb on + strand, within sbcBat 2081.218 kb on - strand, within sbcBat 2081.220 kb on + strand, within sbcBat 2081.238 kb on - strand, within sbcBat 2081.289 kb on - strand, within sbcBat 2081.289 kb on - strand, within sbcBat 2081.300 kb on + strand, within sbcBat 2081.307 kb on + strand, within sbcBat 2081.308 kb on - strand, within sbcBat 2081.308 kb on - strand, within sbcBat 2081.410 kb on - strand, within sbcBat 2081.411 kb on + strand, within sbcBat 2081.411 kb on + strand, within sbcBat 2081.513 kb on + strand, within sbcBat 2081.518 kb on - strand, within sbcB

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ticarcillin 0.000015625 mM
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2,078,611 - -0.5
2,078,684 - -0.2
2,078,689 - -2.2
2,078,853 + +0.4
2,078,861 - sbmC b2009 0.10 +1.0
2,078,864 + sbmC b2009 0.11 -0.3
2,079,000 + sbmC b2009 0.39 +0.5
2,079,077 + sbmC b2009 0.56 +0.3
2,079,281 + +0.4
2,079,472 - +0.2
2,079,472 - -0.6
2,079,505 - -1.7
2,079,568 - dacD b2010 0.14 +0.4
2,079,612 + dacD b2010 0.18 -0.6
2,079,630 + dacD b2010 0.19 -0.7
2,079,784 + dacD b2010 0.32 -0.8
2,079,833 - dacD b2010 0.37 -0.1
2,079,833 - dacD b2010 0.37 -0.1
2,079,857 + dacD b2010 0.39 +0.4
2,079,857 + dacD b2010 0.39 +0.5
2,079,966 + dacD b2010 0.48 +0.7
2,080,001 + dacD b2010 0.51 +1.0
2,080,041 + dacD b2010 0.54 -1.2
2,080,420 + dacD b2010 0.87 -0.4
2,080,420 + dacD b2010 0.87 +0.4
2,080,685 - -1.4
2,080,685 - +0.0
2,080,691 - -0.3
2,080,744 + +1.0
2,080,777 - -0.7
2,080,787 - -0.8
2,080,857 - -1.0
2,080,857 - -1.2
2,080,867 - -1.4
2,080,867 - -0.6
2,080,982 + sbcB b2011 0.14 -0.9
2,080,982 + sbcB b2011 0.14 -0.6
2,080,990 - sbcB b2011 0.15 -0.5
2,081,062 + sbcB b2011 0.20 -0.7
2,081,062 + sbcB b2011 0.20 -1.2
2,081,077 - sbcB b2011 0.21 -0.2
2,081,151 + sbcB b2011 0.26 -0.0
2,081,153 + sbcB b2011 0.26 -0.2
2,081,218 - sbcB b2011 0.31 +0.9
2,081,220 + sbcB b2011 0.31 +0.1
2,081,238 - sbcB b2011 0.32 +0.0
2,081,289 - sbcB b2011 0.36 +0.1
2,081,289 - sbcB b2011 0.36 -0.9
2,081,300 + sbcB b2011 0.36 +0.1
2,081,307 + sbcB b2011 0.37 -0.3
2,081,308 - sbcB b2011 0.37 -0.2
2,081,308 - sbcB b2011 0.37 -0.6
2,081,410 - sbcB b2011 0.44 -0.9
2,081,411 + sbcB b2011 0.44 +0.1
2,081,411 + sbcB b2011 0.44 +0.4
2,081,513 + sbcB b2011 0.51 +0.1
2,081,518 - sbcB b2011 0.52 +1.0

Or see this region's nucleotide sequence