Strain Fitness in Escherichia coli BW25113 around b0090

Experiment: LB Anaerobic with Ticarcillin 0.000015625 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntftsW and murG overlap by 4 nucleotidesmurG and murC are separated by 53 nucleotides b0089: ftsW - integral membrane protein involved in stabilizing FstZ ring during cell division (NCBI), at 98,403 to 99,647 ftsW b0090: murG - N-acetylglucosaminyl transferase (NCBI), at 99,644 to 100,711 murG b0091: murC - UDP-N-acetylmuramate--L-alanine ligase (NCBI), at 100,765 to 102,240 murC Position (kb) 99 100 101Strain fitness (log2 ratio) -1 0 1at 100.705 kb on + strandat 100.709 kb on + strandat 100.709 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ticarcillin 0.000015625 mM
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100,705 + -0.1
100,709 + -0.7
100,709 + +1.6

Or see this region's nucleotide sequence