Strain Fitness in Escherichia coli BW25113 around b0634

Experiment: LB Anaerobic with Ticarcillin 0.00003125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrlpA and mrdB are separated by 10 nucleotidesmrdB and mrdA are separated by 2 nucleotides b0633: rlpA - minor lipoprotein (NCBI), at 663,325 to 664,413 rlpA b0634: mrdB - cell wall shape-determining protein (NCBI), at 664,424 to 665,536 mrdB b0635: mrdA - transpeptidase involved in peptidoglycan synthesis (penicillin-binding protein 2) (NCBI), at 665,539 to 667,440 mrdA Position (kb) 664 665 666Strain fitness (log2 ratio) -2 -1 0 1at 663.427 kb on - strandat 663.465 kb on - strand, within rlpAat 663.465 kb on - strand, within rlpAat 663.475 kb on - strand, within rlpAat 663.532 kb on - strand, within rlpAat 663.581 kb on + strand, within rlpAat 663.581 kb on + strand, within rlpAat 663.620 kb on + strand, within rlpAat 663.697 kb on - strand, within rlpAat 663.770 kb on - strand, within rlpAat 663.775 kb on - strand, within rlpAat 663.775 kb on - strand, within rlpAat 663.844 kb on - strand, within rlpAat 663.849 kb on - strand, within rlpAat 663.849 kb on - strand, within rlpAat 663.860 kb on + strand, within rlpAat 663.865 kb on + strand, within rlpAat 663.865 kb on + strand, within rlpAat 663.867 kb on + strand, within rlpAat 663.873 kb on - strand, within rlpAat 663.894 kb on + strand, within rlpAat 663.900 kb on - strand, within rlpAat 663.900 kb on - strand, within rlpAat 663.941 kb on - strand, within rlpAat 664.174 kb on + strand, within rlpAat 664.260 kb on - strand, within rlpAat 664.260 kb on - strand, within rlpAat 664.293 kb on + strand, within rlpAat 664.293 kb on + strand, within rlpAat 664.303 kb on + strand, within rlpAat 664.311 kb on - strandat 664.323 kb on + strandat 664.348 kb on + strandat 664.367 kb on - strandat 664.385 kb on + strandat 664.385 kb on + strandat 664.413 kb on + strandat 665.612 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Ticarcillin 0.00003125 mM
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663,427 - -0.6
663,465 - rlpA b0633 0.13 -0.6
663,465 - rlpA b0633 0.13 -2.1
663,475 - rlpA b0633 0.14 -2.7
663,532 - rlpA b0633 0.19 -0.7
663,581 + rlpA b0633 0.24 -0.4
663,581 + rlpA b0633 0.24 -0.2
663,620 + rlpA b0633 0.27 +0.1
663,697 - rlpA b0633 0.34 -1.1
663,770 - rlpA b0633 0.41 -0.2
663,775 - rlpA b0633 0.41 -1.1
663,775 - rlpA b0633 0.41 -0.7
663,844 - rlpA b0633 0.48 -0.1
663,849 - rlpA b0633 0.48 -0.3
663,849 - rlpA b0633 0.48 +0.0
663,860 + rlpA b0633 0.49 +0.5
663,865 + rlpA b0633 0.50 +0.3
663,865 + rlpA b0633 0.50 +0.7
663,867 + rlpA b0633 0.50 -1.2
663,873 - rlpA b0633 0.50 -1.0
663,894 + rlpA b0633 0.52 -0.9
663,900 - rlpA b0633 0.53 -1.1
663,900 - rlpA b0633 0.53 -1.0
663,941 - rlpA b0633 0.57 -2.4
664,174 + rlpA b0633 0.78 +0.7
664,260 - rlpA b0633 0.86 -1.4
664,260 - rlpA b0633 0.86 -0.2
664,293 + rlpA b0633 0.89 -1.3
664,293 + rlpA b0633 0.89 -0.3
664,303 + rlpA b0633 0.90 +0.1
664,311 - -0.1
664,323 + +0.9
664,348 + +0.7
664,367 - -1.4
664,385 + -0.3
664,385 + -1.9
664,413 + -0.3
665,612 - +0.3

Or see this region's nucleotide sequence