Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dinD and yicG are separated by 289 nucleotides yicG and yicF overlap by 4 nucleotides yicF and gmk are separated by 257 nucleotides gmk and rpoZ are separated by 54 nucleotides
b3645: dinD - DNA-damage-inducible protein (VIMSS), at 3,815,783 to 3,816,607
dinD
b3646: yicG - orf, hypothetical protein (VIMSS), at 3,816,897 to 3,817,514
yicG
b3647: yicF - putative enzyme (VIMSS), at 3,817,511 to 3,819,193
yicF
b3648: gmk - guanylate kinase (NCBI), at 3,819,451 to 3,820,074
gmk
b3649: rpoZ - DNA-directed RNA polymerase subunit omega (NCBI), at 3,820,129 to 3,820,404
rpoZ
Position (kb)
3817
3818
3819
3820 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3816.531 kb on + strand at 3816.561 kb on - strand at 3816.629 kb on + strand at 3816.632 kb on + strand at 3816.666 kb on + strand at 3816.670 kb on + strand at 3816.866 kb on - strand at 3816.908 kb on - strand at 3816.908 kb on - strand at 3816.908 kb on - strand at 3816.908 kb on - strand at 3816.934 kb on - strand at 3816.938 kb on + strand at 3816.938 kb on + strand at 3816.946 kb on - strand at 3816.947 kb on - strand at 3816.947 kb on - strand at 3816.961 kb on + strand, within yicG at 3816.966 kb on + strand, within yicG at 3816.966 kb on + strand, within yicG at 3816.986 kb on - strand, within yicG at 3816.986 kb on - strand, within yicG at 3817.027 kb on + strand, within yicG at 3817.027 kb on + strand, within yicG at 3817.051 kb on + strand, within yicG at 3817.052 kb on - strand, within yicG at 3817.057 kb on - strand, within yicG at 3817.087 kb on + strand, within yicG at 3817.087 kb on + strand, within yicG at 3817.146 kb on + strand, within yicG at 3817.149 kb on - strand, within yicG at 3817.149 kb on - strand, within yicG at 3817.207 kb on + strand, within yicG at 3817.215 kb on - strand, within yicG at 3817.231 kb on - strand, within yicG at 3817.285 kb on - strand, within yicG at 3817.285 kb on - strand, within yicG at 3817.328 kb on + strand, within yicG at 3817.328 kb on + strand, within yicG at 3817.335 kb on + strand, within yicG at 3817.345 kb on - strand, within yicG at 3817.371 kb on - strand, within yicG at 3817.376 kb on - strand, within yicG at 3817.376 kb on - strand, within yicG at 3817.378 kb on - strand, within yicG at 3817.391 kb on + strand, within yicG at 3817.401 kb on + strand, within yicG at 3817.401 kb on + strand, within yicG at 3817.433 kb on - strand, within yicG at 3817.485 kb on + strand at 3817.506 kb on - strand at 3817.607 kb on + strand at 3817.615 kb on - strand at 3817.615 kb on - strand at 3817.640 kb on - strand at 3817.665 kb on + strand at 3817.670 kb on + strand at 3817.673 kb on - strand at 3817.673 kb on - strand at 3817.708 kb on + strand, within yicF at 3817.716 kb on - strand, within yicF at 3817.795 kb on + strand, within yicF at 3817.795 kb on + strand, within yicF at 3817.828 kb on - strand, within yicF at 3817.828 kb on - strand, within yicF at 3817.877 kb on - strand, within yicF at 3817.877 kb on - strand, within yicF at 3817.897 kb on + strand, within yicF at 3817.900 kb on - strand, within yicF at 3817.918 kb on - strand, within yicF at 3817.918 kb on - strand, within yicF at 3817.939 kb on + strand, within yicF at 3818.035 kb on + strand, within yicF at 3818.043 kb on - strand, within yicF at 3818.043 kb on - strand, within yicF at 3818.225 kb on - strand, within yicF at 3818.255 kb on - strand, within yicF at 3818.255 kb on - strand, within yicF at 3818.289 kb on + strand, within yicF at 3818.304 kb on + strand, within yicF at 3818.375 kb on - strand, within yicF at 3818.375 kb on - strand, within yicF at 3818.388 kb on + strand, within yicF at 3818.390 kb on + strand, within yicF at 3818.400 kb on + strand, within yicF at 3818.400 kb on + strand, within yicF at 3818.439 kb on + strand, within yicF at 3818.496 kb on + strand, within yicF at 3818.564 kb on + strand, within yicF at 3818.627 kb on + strand, within yicF at 3818.672 kb on + strand, within yicF at 3818.672 kb on + strand, within yicF at 3818.675 kb on + strand, within yicF at 3818.680 kb on - strand, within yicF at 3818.735 kb on + strand, within yicF at 3818.743 kb on - strand, within yicF at 3818.781 kb on + strand, within yicF at 3818.794 kb on + strand, within yicF at 3818.794 kb on + strand, within yicF at 3818.806 kb on + strand, within yicF at 3818.832 kb on + strand, within yicF at 3818.840 kb on - strand, within yicF at 3818.886 kb on + strand, within yicF at 3818.899 kb on + strand, within yicF at 3818.904 kb on - strand, within yicF at 3818.920 kb on + strand, within yicF at 3818.938 kb on - strand, within yicF at 3818.994 kb on - strand, within yicF at 3818.994 kb on - strand, within yicF at 3819.005 kb on - strand, within yicF at 3819.005 kb on - strand, within yicF at 3819.041 kb on - strand at 3819.053 kb on - strand at 3819.065 kb on - strand at 3819.111 kb on - strand at 3819.121 kb on + strand at 3819.121 kb on + strand at 3819.130 kb on - strand at 3819.173 kb on - strand at 3819.191 kb on + strand at 3819.197 kb on - strand at 3819.197 kb on - strand at 3819.212 kb on - strand at 3819.234 kb on + strand at 3819.252 kb on - strand at 3819.252 kb on - strand at 3819.303 kb on - strand at 3819.394 kb on - strand at 3819.394 kb on - strand at 3820.087 kb on - strand at 3820.111 kb on - strand at 3820.144 kb on + strand at 3820.144 kb on + strand at 3820.151 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,816,531 + +0.9 3,816,561 - +0.8 3,816,629 + +0.6 3,816,632 + +1.3 3,816,666 + +2.4 3,816,670 + +1.8 3,816,866 - +1.2 3,816,908 - +1.7 3,816,908 - -0.0 3,816,908 - +1.5 3,816,908 - +0.4 3,816,934 - +1.9 3,816,938 + +1.6 3,816,938 + +1.6 3,816,946 - +1.4 3,816,947 - +1.7 3,816,947 - +1.1 3,816,961 + yicG b3646 0.10 +2.6 3,816,966 + yicG b3646 0.11 +2.2 3,816,966 + yicG b3646 0.11 +1.9 3,816,986 - yicG b3646 0.14 +2.6 3,816,986 - yicG b3646 0.14 +2.0 3,817,027 + yicG b3646 0.21 +1.4 3,817,027 + yicG b3646 0.21 +1.7 3,817,051 + yicG b3646 0.25 +0.6 3,817,052 - yicG b3646 0.25 +0.9 3,817,057 - yicG b3646 0.26 +0.0 3,817,087 + yicG b3646 0.31 +3.1 3,817,087 + yicG b3646 0.31 +2.0 3,817,146 + yicG b3646 0.40 +0.7 3,817,149 - yicG b3646 0.41 +1.6 3,817,149 - yicG b3646 0.41 +1.3 3,817,207 + yicG b3646 0.50 +0.4 3,817,215 - yicG b3646 0.51 +1.7 3,817,231 - yicG b3646 0.54 +2.8 3,817,285 - yicG b3646 0.63 +1.7 3,817,285 - yicG b3646 0.63 +1.2 3,817,328 + yicG b3646 0.70 +1.2 3,817,328 + yicG b3646 0.70 +1.6 3,817,335 + yicG b3646 0.71 +1.4 3,817,345 - yicG b3646 0.72 +1.8 3,817,371 - yicG b3646 0.77 +0.9 3,817,376 - yicG b3646 0.78 +2.2 3,817,376 - yicG b3646 0.78 +0.6 3,817,378 - yicG b3646 0.78 +1.3 3,817,391 + yicG b3646 0.80 +1.3 3,817,401 + yicG b3646 0.82 +2.9 3,817,401 + yicG b3646 0.82 +1.9 3,817,433 - yicG b3646 0.87 +1.7 3,817,485 + +1.3 3,817,506 - +1.6 3,817,607 + -1.0 3,817,615 - +1.1 3,817,615 - +2.1 3,817,640 - +0.4 3,817,665 + +0.2 3,817,670 + -0.4 3,817,673 - +1.2 3,817,673 - +1.1 3,817,708 + yicF b3647 0.12 +0.1 3,817,716 - yicF b3647 0.12 +2.4 3,817,795 + yicF b3647 0.17 +2.0 3,817,795 + yicF b3647 0.17 -2.7 3,817,828 - yicF b3647 0.19 +0.7 3,817,828 - yicF b3647 0.19 +4.2 3,817,877 - yicF b3647 0.22 +0.5 3,817,877 - yicF b3647 0.22 +1.0 3,817,897 + yicF b3647 0.23 +0.6 3,817,900 - yicF b3647 0.23 +0.6 3,817,918 - yicF b3647 0.24 +1.5 3,817,918 - yicF b3647 0.24 -0.3 3,817,939 + yicF b3647 0.25 -0.8 3,818,035 + yicF b3647 0.31 -1.3 3,818,043 - yicF b3647 0.32 +0.7 3,818,043 - yicF b3647 0.32 -0.2 3,818,225 - yicF b3647 0.42 -0.0 3,818,255 - yicF b3647 0.44 +0.8 3,818,255 - yicF b3647 0.44 +0.3 3,818,289 + yicF b3647 0.46 +1.4 3,818,304 + yicF b3647 0.47 +0.1 3,818,375 - yicF b3647 0.51 -0.3 3,818,375 - yicF b3647 0.51 +0.3 3,818,388 + yicF b3647 0.52 +0.7 3,818,390 + yicF b3647 0.52 +0.4 3,818,400 + yicF b3647 0.53 -0.6 3,818,400 + yicF b3647 0.53 +1.0 3,818,439 + yicF b3647 0.55 +0.6 3,818,496 + yicF b3647 0.59 +1.2 3,818,564 + yicF b3647 0.63 +0.6 3,818,627 + yicF b3647 0.66 -1.6 3,818,672 + yicF b3647 0.69 -0.6 3,818,672 + yicF b3647 0.69 -1.2 3,818,675 + yicF b3647 0.69 -0.5 3,818,680 - yicF b3647 0.69 +0.4 3,818,735 + yicF b3647 0.73 -1.1 3,818,743 - yicF b3647 0.73 +0.9 3,818,781 + yicF b3647 0.75 -0.4 3,818,794 + yicF b3647 0.76 -0.0 3,818,794 + yicF b3647 0.76 +1.0 3,818,806 + yicF b3647 0.77 -2.0 3,818,832 + yicF b3647 0.78 -0.4 3,818,840 - yicF b3647 0.79 +1.1 3,818,886 + yicF b3647 0.82 +1.0 3,818,899 + yicF b3647 0.82 +0.8 3,818,904 - yicF b3647 0.83 +1.1 3,818,920 + yicF b3647 0.84 -1.3 3,818,938 - yicF b3647 0.85 +0.8 3,818,994 - yicF b3647 0.88 +1.0 3,818,994 - yicF b3647 0.88 +0.1 3,819,005 - yicF b3647 0.89 +1.1 3,819,005 - yicF b3647 0.89 +1.0 3,819,041 - +1.8 3,819,053 - +1.1 3,819,065 - +0.5 3,819,111 - +0.5 3,819,121 + +1.0 3,819,121 + +0.4 3,819,130 - +0.9 3,819,173 - +1.2 3,819,191 + +0.3 3,819,197 - +3.4 3,819,197 - +3.1 3,819,212 - +0.3 3,819,234 + -0.2 3,819,252 - +0.8 3,819,252 - +0.2 3,819,303 - -1.2 3,819,394 - +1.8 3,819,394 - +2.4 3,820,087 - -3.2 3,820,111 - +0.4 3,820,144 + -1.0 3,820,144 + -1.6 3,820,151 + -0.6
Or see this region's nucleotide sequence