Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yhiP and yhiQ are separated by 48 nucleotides yhiQ and prlC are separated by 7 nucleotides
b3496: yhiP - predicted transporter (NCBI), at 3,638,885 to 3,640,354
yhiP
b3497: yhiQ - predicted SAM-dependent methyltransferase (NCBI), at 3,640,403 to 3,641,155
yhiQ
b3498: prlC - oligopeptidase A (NCBI), at 3,641,163 to 3,643,205
prlC
Position (kb)
3640
3641
3642 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3639.408 kb on + strand, within yhiP at 3639.408 kb on + strand, within yhiP at 3639.428 kb on + strand, within yhiP at 3639.448 kb on + strand, within yhiP at 3639.448 kb on + strand, within yhiP at 3639.456 kb on - strand, within yhiP at 3639.456 kb on - strand, within yhiP at 3639.481 kb on - strand, within yhiP at 3639.534 kb on - strand, within yhiP at 3639.585 kb on + strand, within yhiP at 3639.593 kb on - strand, within yhiP at 3639.704 kb on - strand, within yhiP at 3639.704 kb on - strand, within yhiP at 3639.747 kb on + strand at 3639.747 kb on + strand, within yhiP at 3639.836 kb on + strand, within yhiP at 3639.859 kb on - strand, within yhiP at 3639.859 kb on - strand, within yhiP at 3639.861 kb on + strand, within yhiP at 3639.861 kb on + strand, within yhiP at 3639.869 kb on - strand, within yhiP at 3639.869 kb on - strand, within yhiP at 3639.876 kb on + strand, within yhiP at 3639.892 kb on + strand, within yhiP at 3639.892 kb on + strand, within yhiP at 3639.900 kb on - strand, within yhiP at 3639.937 kb on + strand, within yhiP at 3639.937 kb on + strand, within yhiP at 3640.007 kb on - strand, within yhiP at 3640.007 kb on - strand, within yhiP at 3640.020 kb on - strand, within yhiP at 3640.026 kb on + strand, within yhiP at 3640.051 kb on + strand, within yhiP at 3640.058 kb on + strand, within yhiP at 3640.085 kb on - strand, within yhiP at 3640.095 kb on + strand, within yhiP at 3640.105 kb on + strand, within yhiP at 3640.114 kb on - strand, within yhiP at 3640.225 kb on + strand at 3640.235 kb on + strand at 3640.235 kb on + strand at 3640.237 kb on + strand at 3640.241 kb on + strand at 3640.241 kb on + strand at 3640.253 kb on - strand at 3640.271 kb on - strand at 3640.290 kb on - strand at 3640.292 kb on + strand at 3640.295 kb on - strand at 3640.298 kb on - strand at 3640.303 kb on + strand at 3640.326 kb on - strand at 3640.326 kb on - strand at 3640.347 kb on + strand at 3640.368 kb on + strand at 3640.376 kb on - strand at 3640.378 kb on + strand at 3640.416 kb on + strand at 3640.416 kb on + strand at 3640.416 kb on + strand at 3640.416 kb on + strand at 3640.436 kb on - strand at 3640.488 kb on + strand, within yhiQ at 3640.488 kb on + strand, within yhiQ at 3640.510 kb on - strand, within yhiQ at 3640.627 kb on - strand, within yhiQ at 3640.627 kb on - strand, within yhiQ at 3640.641 kb on - strand, within yhiQ at 3640.664 kb on - strand, within yhiQ at 3640.664 kb on - strand, within yhiQ at 3640.669 kb on + strand, within yhiQ at 3640.677 kb on - strand, within yhiQ at 3640.677 kb on - strand, within yhiQ at 3640.677 kb on - strand, within yhiQ at 3640.677 kb on - strand, within yhiQ at 3640.677 kb on - strand, within yhiQ at 3640.687 kb on + strand, within yhiQ at 3640.687 kb on + strand, within yhiQ at 3640.729 kb on - strand, within yhiQ at 3640.731 kb on - strand, within yhiQ at 3640.781 kb on - strand, within yhiQ at 3640.802 kb on - strand, within yhiQ at 3640.830 kb on + strand, within yhiQ at 3640.835 kb on + strand, within yhiQ at 3640.848 kb on - strand, within yhiQ at 3640.861 kb on + strand, within yhiQ at 3640.903 kb on - strand, within yhiQ at 3640.932 kb on - strand, within yhiQ at 3641.043 kb on + strand, within yhiQ at 3641.071 kb on - strand, within yhiQ at 3641.078 kb on + strand, within yhiQ at 3641.119 kb on + strand at 3641.166 kb on + strand at 3641.166 kb on + strand at 3641.174 kb on - strand at 3641.174 kb on - strand at 3641.174 kb on - strand at 3641.199 kb on - strand at 3641.215 kb on - strand at 3641.224 kb on + strand at 3641.368 kb on + strand, within prlC at 3641.376 kb on - strand, within prlC at 3641.378 kb on - strand, within prlC at 3641.530 kb on + strand, within prlC at 3641.530 kb on + strand, within prlC at 3641.573 kb on + strand, within prlC at 3641.573 kb on - strand, within prlC at 3641.573 kb on - strand, within prlC at 3641.643 kb on - strand, within prlC at 3641.689 kb on + strand, within prlC at 3641.702 kb on - strand, within prlC at 3641.771 kb on + strand, within prlC at 3641.771 kb on + strand, within prlC at 3641.772 kb on - strand, within prlC at 3641.779 kb on - strand, within prlC at 3641.779 kb on - strand, within prlC at 3641.880 kb on - strand, within prlC at 3641.934 kb on + strand, within prlC at 3641.938 kb on + strand, within prlC at 3641.988 kb on - strand, within prlC at 3641.988 kb on - strand, within prlC at 3641.989 kb on + strand, within prlC at 3642.100 kb on + strand, within prlC at 3642.100 kb on + strand, within prlC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,639,408 + yhiP b3496 0.36 -0.1 3,639,408 + yhiP b3496 0.36 -0.2 3,639,428 + yhiP b3496 0.37 -1.4 3,639,448 + yhiP b3496 0.38 +2.3 3,639,448 + yhiP b3496 0.38 +3.4 3,639,456 - yhiP b3496 0.39 -1.3 3,639,456 - yhiP b3496 0.39 -2.6 3,639,481 - yhiP b3496 0.41 +0.2 3,639,534 - yhiP b3496 0.44 -1.4 3,639,585 + yhiP b3496 0.48 -1.2 3,639,593 - yhiP b3496 0.48 -0.6 3,639,704 - yhiP b3496 0.56 -2.8 3,639,704 - yhiP b3496 0.56 -0.2 3,639,747 + -1.2 3,639,747 + yhiP b3496 0.59 -0.6 3,639,836 + yhiP b3496 0.65 +0.4 3,639,859 - yhiP b3496 0.66 -1.4 3,639,859 - yhiP b3496 0.66 +0.2 3,639,861 + yhiP b3496 0.66 -0.3 3,639,861 + yhiP b3496 0.66 +0.7 3,639,869 - yhiP b3496 0.67 -0.7 3,639,869 - yhiP b3496 0.67 -0.0 3,639,876 + yhiP b3496 0.67 -0.2 3,639,892 + yhiP b3496 0.69 +0.3 3,639,892 + yhiP b3496 0.69 +0.4 3,639,900 - yhiP b3496 0.69 +0.4 3,639,937 + yhiP b3496 0.72 +0.6 3,639,937 + yhiP b3496 0.72 +0.1 3,640,007 - yhiP b3496 0.76 -2.7 3,640,007 - yhiP b3496 0.76 -2.3 3,640,020 - yhiP b3496 0.77 +0.8 3,640,026 + yhiP b3496 0.78 -1.4 3,640,051 + yhiP b3496 0.79 -0.5 3,640,058 + yhiP b3496 0.80 -0.2 3,640,085 - yhiP b3496 0.82 +0.4 3,640,095 + yhiP b3496 0.82 -1.2 3,640,105 + yhiP b3496 0.83 +0.8 3,640,114 - yhiP b3496 0.84 -0.9 3,640,225 + +0.1 3,640,235 + -0.2 3,640,235 + -0.9 3,640,237 + -1.2 3,640,241 + -1.6 3,640,241 + +1.7 3,640,253 - +0.2 3,640,271 - -2.6 3,640,290 - +0.6 3,640,292 + -2.7 3,640,295 - -1.7 3,640,298 - -0.3 3,640,303 + -1.3 3,640,326 - -1.9 3,640,326 - -0.8 3,640,347 + -1.2 3,640,368 + +0.9 3,640,376 - -0.9 3,640,378 + -0.7 3,640,416 + -1.2 3,640,416 + -0.4 3,640,416 + +0.2 3,640,416 + -1.2 3,640,436 - -0.6 3,640,488 + yhiQ b3497 0.11 -0.4 3,640,488 + yhiQ b3497 0.11 -0.2 3,640,510 - yhiQ b3497 0.14 -1.3 3,640,627 - yhiQ b3497 0.30 -1.2 3,640,627 - yhiQ b3497 0.30 -0.6 3,640,641 - yhiQ b3497 0.32 -0.5 3,640,664 - yhiQ b3497 0.35 -1.1 3,640,664 - yhiQ b3497 0.35 -2.3 3,640,669 + yhiQ b3497 0.35 -3.1 3,640,677 - yhiQ b3497 0.36 +0.4 3,640,677 - yhiQ b3497 0.36 +1.4 3,640,677 - yhiQ b3497 0.36 +1.1 3,640,677 - yhiQ b3497 0.36 -2.7 3,640,677 - yhiQ b3497 0.36 -1.7 3,640,687 + yhiQ b3497 0.38 -1.4 3,640,687 + yhiQ b3497 0.38 -2.2 3,640,729 - yhiQ b3497 0.43 +0.3 3,640,731 - yhiQ b3497 0.44 -0.6 3,640,781 - yhiQ b3497 0.50 -1.4 3,640,802 - yhiQ b3497 0.53 -0.4 3,640,830 + yhiQ b3497 0.57 -1.4 3,640,835 + yhiQ b3497 0.57 -1.1 3,640,848 - yhiQ b3497 0.59 -1.8 3,640,861 + yhiQ b3497 0.61 -0.2 3,640,903 - yhiQ b3497 0.66 -0.6 3,640,932 - yhiQ b3497 0.70 -0.3 3,641,043 + yhiQ b3497 0.85 -1.4 3,641,071 - yhiQ b3497 0.89 -0.6 3,641,078 + yhiQ b3497 0.90 +0.0 3,641,119 + -1.3 3,641,166 + -1.1 3,641,166 + -1.5 3,641,174 - +0.8 3,641,174 - -1.1 3,641,174 - -0.4 3,641,199 - +0.0 3,641,215 - -0.8 3,641,224 + +1.6 3,641,368 + prlC b3498 0.10 +0.7 3,641,376 - prlC b3498 0.10 +0.3 3,641,378 - prlC b3498 0.11 -2.0 3,641,530 + prlC b3498 0.18 -0.9 3,641,530 + prlC b3498 0.18 -0.5 3,641,573 + prlC b3498 0.20 -2.6 3,641,573 - prlC b3498 0.20 +0.2 3,641,573 - prlC b3498 0.20 -1.9 3,641,643 - prlC b3498 0.23 -1.6 3,641,689 + prlC b3498 0.26 +0.2 3,641,702 - prlC b3498 0.26 -1.0 3,641,771 + prlC b3498 0.30 -1.6 3,641,771 + prlC b3498 0.30 -0.7 3,641,772 - prlC b3498 0.30 -1.2 3,641,779 - prlC b3498 0.30 +0.0 3,641,779 - prlC b3498 0.30 +0.4 3,641,880 - prlC b3498 0.35 -2.7 3,641,934 + prlC b3498 0.38 -1.6 3,641,938 + prlC b3498 0.38 +0.6 3,641,988 - prlC b3498 0.40 +2.0 3,641,988 - prlC b3498 0.40 -0.6 3,641,989 + prlC b3498 0.40 +0.3 3,642,100 + prlC b3498 0.46 -1.0 3,642,100 + prlC b3498 0.46 -0.0
Or see this region's nucleotide sequence