Strain Fitness in Escherichia coli BW25113 around b3464

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntftsX and ftsE overlap by 8 nucleotidesftsE and ftsY are separated by 2 nucleotidesftsY and yhhF are separated by 149 nucleotidesyhhF and yhhL overlap by 11 nucleotides b3462: ftsX - predicted transporter subunit: membrane component of ABC superfamily (NCBI), at 3,599,051 to 3,600,109 ftsX b3463: ftsE - predicted transporter subunit: ATP-binding component of ABC superfamily (NCBI), at 3,600,102 to 3,600,770 ftsE b3464: ftsY - fused Signal Recognition Particle (SRP) receptor: membrane binding protein/conserved protein (NCBI), at 3,600,773 to 3,602,266 ftsY b3465: yhhF - predicted methyltransferase (NCBI), at 3,602,416 to 3,603,012 yhhF b3466: yhhL - conserved inner membrane protein (NCBI), at 3,603,002 to 3,603,271 yhhL Position (kb) 3600 3601 3602 3603Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 3599.832 kb on + strand, within ftsXat 3599.840 kb on - strand, within ftsXat 3599.945 kb on - strand, within ftsXat 3599.945 kb on - strand, within ftsXat 3599.972 kb on - strand, within ftsXat 3600.020 kb on + strandat 3600.042 kb on + strandat 3600.042 kb on + strandat 3600.106 kb on - strandat 3600.117 kb on + strandat 3600.126 kb on - strandat 3600.126 kb on - strandat 3600.145 kb on - strandat 3600.147 kb on - strandat 3600.147 kb on - strandat 3600.157 kb on - strandat 3600.184 kb on - strand, within ftsEat 3600.286 kb on + strand, within ftsEat 3600.304 kb on + strand, within ftsEat 3600.309 kb on + strand, within ftsEat 3600.412 kb on + strand, within ftsEat 3600.451 kb on + strand, within ftsEat 3600.485 kb on - strand, within ftsEat 3600.490 kb on - strand, within ftsEat 3600.604 kb on + strand, within ftsEat 3600.612 kb on - strand, within ftsEat 3600.692 kb on + strand, within ftsEat 3600.692 kb on + strand, within ftsEat 3600.719 kb on - strandat 3602.144 kb on - strandat 3602.314 kb on + strandat 3602.314 kb on + strandat 3602.321 kb on - strandat 3602.324 kb on - strandat 3602.358 kb on + strandat 3602.358 kb on + strandat 3602.377 kb on + strandat 3602.377 kb on + strandat 3602.509 kb on - strand, within yhhFat 3602.509 kb on - strand, within yhhFat 3602.519 kb on - strand, within yhhFat 3602.519 kb on - strand, within yhhFat 3602.522 kb on - strand, within yhhFat 3602.522 kb on - strand, within yhhFat 3602.522 kb on - strand, within yhhFat 3602.592 kb on - strand, within yhhFat 3602.621 kb on - strandat 3602.634 kb on - strand, within yhhFat 3602.713 kb on - strand, within yhhFat 3602.736 kb on - strand, within yhhFat 3602.773 kb on - strand, within yhhFat 3602.780 kb on - strand, within yhhFat 3602.780 kb on - strand, within yhhFat 3602.783 kb on - strand, within yhhFat 3602.882 kb on - strand, within yhhFat 3602.907 kb on + strand, within yhhFat 3602.947 kb on - strand, within yhhFat 3602.992 kb on - strandat 3602.993 kb on + strandat 3603.015 kb on - strandat 3603.062 kb on - strand, within yhhLat 3603.062 kb on - strand, within yhhLat 3603.065 kb on + strand, within yhhLat 3603.065 kb on + strand, within yhhLat 3603.083 kb on - strand, within yhhLat 3603.134 kb on + strand, within yhhLat 3603.134 kb on + strand, within yhhLat 3603.135 kb on + strand, within yhhLat 3603.135 kb on + strand, within yhhLat 3603.143 kb on - strand, within yhhLat 3603.159 kb on - strand, within yhhLat 3603.225 kb on + strand, within yhhLat 3603.247 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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3,599,832 + ftsX b3462 0.74 +1.4
3,599,840 - ftsX b3462 0.75 +1.4
3,599,945 - ftsX b3462 0.84 -2.5
3,599,945 - ftsX b3462 0.84 -3.3
3,599,972 - ftsX b3462 0.87 -2.1
3,600,020 + +1.4
3,600,042 + -1.1
3,600,042 + -2.0
3,600,106 - -2.9
3,600,117 + +1.4
3,600,126 - -0.7
3,600,126 - -1.4
3,600,145 - -1.4
3,600,147 - +1.4
3,600,147 - +0.4
3,600,157 - +1.4
3,600,184 - ftsE b3463 0.12 +1.4
3,600,286 + ftsE b3463 0.28 +1.4
3,600,304 + ftsE b3463 0.30 +1.4
3,600,309 + ftsE b3463 0.31 +1.4
3,600,412 + ftsE b3463 0.46 +1.4
3,600,451 + ftsE b3463 0.52 +1.4
3,600,485 - ftsE b3463 0.57 +1.4
3,600,490 - ftsE b3463 0.58 +1.4
3,600,604 + ftsE b3463 0.75 +1.4
3,600,612 - ftsE b3463 0.76 +1.4
3,600,692 + ftsE b3463 0.88 +1.4
3,600,692 + ftsE b3463 0.88 +1.4
3,600,719 - +1.4
3,602,144 - +1.4
3,602,314 + -1.4
3,602,314 + -1.4
3,602,321 - +0.1
3,602,324 - -1.1
3,602,358 + -1.7
3,602,358 + +0.2
3,602,377 + +0.1
3,602,377 + -1.4
3,602,509 - yhhF b3465 0.16 +2.9
3,602,509 - yhhF b3465 0.16 +2.2
3,602,519 - yhhF b3465 0.17 +1.2
3,602,519 - yhhF b3465 0.17 +0.9
3,602,522 - yhhF b3465 0.18 +1.2
3,602,522 - yhhF b3465 0.18 +1.9
3,602,522 - yhhF b3465 0.18 +2.3
3,602,592 - yhhF b3465 0.29 +1.1
3,602,621 - +1.4
3,602,634 - yhhF b3465 0.37 +0.7
3,602,713 - yhhF b3465 0.50 -0.1
3,602,736 - yhhF b3465 0.54 +2.1
3,602,773 - yhhF b3465 0.60 +1.4
3,602,780 - yhhF b3465 0.61 +1.4
3,602,780 - yhhF b3465 0.61 +1.7
3,602,783 - yhhF b3465 0.61 +2.6
3,602,882 - yhhF b3465 0.78 +2.1
3,602,907 + yhhF b3465 0.82 +1.0
3,602,947 - yhhF b3465 0.89 -1.1
3,602,992 - +1.7
3,602,993 + +0.9
3,603,015 - +0.4
3,603,062 - yhhL b3466 0.22 -0.6
3,603,062 - yhhL b3466 0.22 +0.4
3,603,065 + yhhL b3466 0.23 -0.9
3,603,065 + yhhL b3466 0.23 +1.5
3,603,083 - yhhL b3466 0.30 +0.9
3,603,134 + yhhL b3466 0.49 -1.8
3,603,134 + yhhL b3466 0.49 -1.0
3,603,135 + yhhL b3466 0.49 -2.4
3,603,135 + yhhL b3466 0.49 -0.2
3,603,143 - yhhL b3466 0.52 -0.2
3,603,159 - yhhL b3466 0.58 +0.4
3,603,225 + yhhL b3466 0.83 -0.7
3,603,247 - -2.5

Or see this region's nucleotide sequence