Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yqeA and yqeB are separated by 220 nucleotides yqeB and b2876 are separated by 47 nucleotides b2876 and ygfJ are separated by 102 nucleotides
b2874: yqeA - predicted amino acid kinase (NCBI), at 3,009,483 to 3,010,415
yqeA
b2875: yqeB - conserved protein with NAD(P)-binding Rossman fold (NCBI), at 3,010,636 to 3,012,261
yqeB
b2876: b2876 - orf, hypothetical protein (VIMSS), at 3,012,309 to 3,013,079
b2876
b2877: ygfJ - hypothetical protein (NCBI), at 3,013,182 to 3,013,760
ygfJ
Position (kb)
3010
3011
3012
3013 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3009.654 kb on + strand, within yqeA at 3009.688 kb on + strand, within yqeA at 3009.701 kb on - strand, within yqeA at 3009.701 kb on - strand, within yqeA at 3009.730 kb on + strand, within yqeA at 3009.816 kb on - strand, within yqeA at 3009.822 kb on - strand, within yqeA at 3009.922 kb on - strand, within yqeA at 3010.002 kb on + strand, within yqeA at 3010.013 kb on + strand, within yqeA at 3010.232 kb on + strand, within yqeA at 3010.232 kb on + strand, within yqeA at 3010.280 kb on + strand, within yqeA at 3010.282 kb on + strand, within yqeA at 3010.282 kb on + strand, within yqeA at 3010.349 kb on + strand at 3010.390 kb on + strand at 3010.590 kb on + strand at 3010.596 kb on - strand at 3010.610 kb on + strand at 3010.618 kb on - strand at 3010.618 kb on - strand at 3010.697 kb on - strand at 3010.803 kb on + strand, within yqeB at 3010.822 kb on - strand, within yqeB at 3010.822 kb on - strand, within yqeB at 3010.914 kb on - strand, within yqeB at 3010.941 kb on + strand, within yqeB at 3010.952 kb on - strand, within yqeB at 3010.964 kb on - strand, within yqeB at 3010.980 kb on - strand, within yqeB at 3011.204 kb on - strand, within yqeB at 3011.209 kb on + strand, within yqeB at 3011.418 kb on - strand, within yqeB at 3011.469 kb on + strand, within yqeB at 3011.537 kb on + strand, within yqeB at 3011.560 kb on + strand, within yqeB at 3011.597 kb on - strand, within yqeB at 3011.616 kb on - strand, within yqeB at 3011.706 kb on - strand, within yqeB at 3011.918 kb on - strand, within yqeB at 3011.918 kb on - strand, within yqeB at 3011.971 kb on + strand, within yqeB at 3011.971 kb on + strand, within yqeB at 3012.000 kb on - strand, within yqeB at 3012.020 kb on - strand at 3012.020 kb on - strand, within yqeB at 3012.022 kb on + strand, within yqeB at 3012.079 kb on - strand, within yqeB at 3012.079 kb on - strand, within yqeB at 3012.102 kb on - strand at 3012.147 kb on - strand at 3012.283 kb on - strand at 3012.304 kb on + strand at 3012.378 kb on + strand at 3012.497 kb on - strand, within b2876 at 3012.666 kb on - strand, within b2876 at 3012.666 kb on - strand, within b2876 at 3012.715 kb on + strand, within b2876 at 3012.848 kb on + strand, within b2876 at 3012.888 kb on + strand, within b2876 at 3012.888 kb on + strand, within b2876 at 3012.939 kb on + strand, within b2876 at 3012.947 kb on - strand, within b2876 at 3012.954 kb on + strand, within b2876 at 3013.010 kb on + strand at 3013.010 kb on + strand at 3013.012 kb on - strand at 3013.012 kb on - strand at 3013.026 kb on + strand at 3013.047 kb on + strand at 3013.047 kb on + strand at 3013.052 kb on + strand at 3013.052 kb on + strand at 3013.084 kb on + strand at 3013.121 kb on + strand at 3013.124 kb on - strand at 3013.125 kb on + strand at 3013.138 kb on + strand at 3013.139 kb on + strand at 3013.156 kb on - strand at 3013.166 kb on + strand at 3013.169 kb on - strand at 3013.177 kb on + strand at 3013.177 kb on + strand at 3013.184 kb on + strand at 3013.196 kb on - strand at 3013.198 kb on + strand at 3013.203 kb on - strand at 3013.228 kb on - strand at 3013.228 kb on - strand at 3013.236 kb on - strand at 3013.238 kb on + strand at 3013.241 kb on + strand, within ygfJ at 3013.242 kb on + strand, within ygfJ at 3013.248 kb on - strand, within ygfJ at 3013.253 kb on + strand, within ygfJ at 3013.253 kb on + strand, within ygfJ at 3013.254 kb on + strand, within ygfJ at 3013.254 kb on + strand, within ygfJ at 3013.254 kb on + strand, within ygfJ at 3013.256 kb on - strand, within ygfJ
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 3,009,654 + yqeA b2874 0.18 +1.2 3,009,688 + yqeA b2874 0.22 +0.2 3,009,701 - yqeA b2874 0.23 +0.8 3,009,701 - yqeA b2874 0.23 -0.5 3,009,730 + yqeA b2874 0.26 -0.8 3,009,816 - yqeA b2874 0.36 -0.7 3,009,822 - yqeA b2874 0.36 -0.6 3,009,922 - yqeA b2874 0.47 +0.6 3,010,002 + yqeA b2874 0.56 +0.0 3,010,013 + yqeA b2874 0.57 -0.5 3,010,232 + yqeA b2874 0.80 +0.4 3,010,232 + yqeA b2874 0.80 +0.2 3,010,280 + yqeA b2874 0.85 -2.3 3,010,282 + yqeA b2874 0.86 -0.4 3,010,282 + yqeA b2874 0.86 -0.6 3,010,349 + +0.2 3,010,390 + -3.2 3,010,590 + +0.8 3,010,596 - -1.3 3,010,610 + -0.5 3,010,618 - -0.7 3,010,618 - +0.3 3,010,697 - +0.1 3,010,803 + yqeB b2875 0.10 -0.7 3,010,822 - yqeB b2875 0.11 -0.3 3,010,822 - yqeB b2875 0.11 -0.1 3,010,914 - yqeB b2875 0.17 -1.9 3,010,941 + yqeB b2875 0.19 -1.1 3,010,952 - yqeB b2875 0.19 +0.1 3,010,964 - yqeB b2875 0.20 +0.8 3,010,980 - yqeB b2875 0.21 -2.1 3,011,204 - yqeB b2875 0.35 -0.2 3,011,209 + yqeB b2875 0.35 +0.4 3,011,418 - yqeB b2875 0.48 -0.4 3,011,469 + yqeB b2875 0.51 -1.6 3,011,537 + yqeB b2875 0.55 -1.6 3,011,560 + yqeB b2875 0.57 +0.1 3,011,597 - yqeB b2875 0.59 +0.3 3,011,616 - yqeB b2875 0.60 -1.1 3,011,706 - yqeB b2875 0.66 -1.2 3,011,918 - yqeB b2875 0.79 +0.5 3,011,918 - yqeB b2875 0.79 -1.7 3,011,971 + yqeB b2875 0.82 +1.1 3,011,971 + yqeB b2875 0.82 +0.1 3,012,000 - yqeB b2875 0.84 -3.5 3,012,020 - +0.5 3,012,020 - yqeB b2875 0.85 -0.2 3,012,022 + yqeB b2875 0.85 +0.5 3,012,079 - yqeB b2875 0.89 -0.2 3,012,079 - yqeB b2875 0.89 +1.0 3,012,102 - -1.6 3,012,147 - -0.5 3,012,283 - -0.1 3,012,304 + -1.3 3,012,378 + -1.5 3,012,497 - b2876 b2876 0.24 -0.1 3,012,666 - b2876 b2876 0.46 +0.0 3,012,666 - b2876 b2876 0.46 +0.1 3,012,715 + b2876 b2876 0.53 -0.1 3,012,848 + b2876 b2876 0.70 +0.8 3,012,888 + b2876 b2876 0.75 -0.9 3,012,888 + b2876 b2876 0.75 -1.1 3,012,939 + b2876 b2876 0.82 -2.2 3,012,947 - b2876 b2876 0.83 +1.1 3,012,954 + b2876 b2876 0.84 +0.8 3,013,010 + -1.7 3,013,010 + -0.1 3,013,012 - +1.1 3,013,012 - +1.1 3,013,026 + +0.1 3,013,047 + -0.0 3,013,047 + -0.5 3,013,052 + +0.5 3,013,052 + +0.3 3,013,084 + +0.2 3,013,121 + +0.7 3,013,124 - +1.1 3,013,125 + -1.5 3,013,138 + -0.3 3,013,139 + -2.3 3,013,156 - +0.1 3,013,166 + +0.4 3,013,169 - -0.5 3,013,177 + +1.1 3,013,177 + -0.5 3,013,184 + +0.4 3,013,196 - +1.1 3,013,198 + -0.5 3,013,203 - -0.5 3,013,228 - -0.9 3,013,228 - -0.5 3,013,236 - +1.1 3,013,238 + -0.9 3,013,241 + ygfJ b2877 0.10 -1.9 3,013,242 + ygfJ b2877 0.10 +0.8 3,013,248 - ygfJ b2877 0.11 +1.1 3,013,253 + ygfJ b2877 0.12 -0.1 3,013,253 + ygfJ b2877 0.12 -1.7 3,013,254 + ygfJ b2877 0.12 +1.9 3,013,254 + ygfJ b2877 0.12 +1.1 3,013,254 + ygfJ b2877 0.12 +0.8 3,013,256 - ygfJ b2877 0.13 -0.5
Or see this region's nucleotide sequence