Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yohD and yohF are separated by 129 nucleotides yohF and mdtQ are separated by 52 nucleotides mdtQ and yohP are separated by 223 nucleotides yohP and dusC are separated by 372 nucleotides
b2136: yohD - orf, hypothetical protein (VIMSS), at 2,223,823 to 2,224,401
yohD
b2137: yohF - predicted oxidoreductase with NAD(P)-binding Rossmann-fold domain (NCBI), at 2,224,531 to 2,225,292
yohF
b2139: mdtQ - orf, hypothetical protein (VIMSS), at 2,225,345 to 2,226,780
mdtQ
b4679: yohP - expressed protein (RefSeq), at 2,227,004 to 2,227,087
yohP
b2140: dusC - tRNA-dihydrouridine synthase C (NCBI), at 2,227,460 to 2,228,407
dusC
Position (kb)
2225
2226
2227 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2224.348 kb on - strand at 2224.358 kb on + strand at 2224.417 kb on - strand at 2224.529 kb on - strand at 2224.541 kb on - strand at 2224.578 kb on + strand at 2224.713 kb on - strand, within yohF at 2224.872 kb on - strand, within yohF at 2224.902 kb on + strand, within yohF at 2224.902 kb on + strand, within yohF at 2224.942 kb on + strand, within yohF at 2224.992 kb on - strand, within yohF at 2224.992 kb on - strand, within yohF at 2224.994 kb on - strand, within yohF at 2224.994 kb on - strand, within yohF at 2225.063 kb on - strand, within yohF at 2225.103 kb on - strand, within yohF at 2225.133 kb on + strand, within yohF at 2225.163 kb on + strand, within yohF at 2225.199 kb on - strand, within yohF at 2225.292 kb on - strand at 2225.409 kb on + strand at 2225.429 kb on - strand at 2225.476 kb on - strand at 2225.538 kb on + strand, within mdtQ at 2225.672 kb on - strand, within mdtQ at 2225.711 kb on + strand, within mdtQ at 2225.719 kb on - strand, within mdtQ at 2225.756 kb on - strand, within mdtQ at 2225.756 kb on - strand, within mdtQ at 2225.777 kb on + strand, within mdtQ at 2225.795 kb on + strand, within mdtQ at 2225.795 kb on + strand, within mdtQ at 2225.843 kb on + strand, within mdtQ at 2225.862 kb on + strand, within mdtQ at 2225.862 kb on + strand, within mdtQ at 2225.867 kb on - strand, within mdtQ at 2225.899 kb on + strand, within mdtQ at 2225.899 kb on + strand, within mdtQ at 2225.906 kb on + strand, within mdtQ at 2225.917 kb on - strand, within mdtQ at 2225.964 kb on - strand, within mdtQ at 2225.964 kb on - strand, within mdtQ at 2225.970 kb on + strand, within mdtQ at 2226.046 kb on - strand, within mdtQ at 2226.046 kb on - strand, within mdtQ at 2226.097 kb on + strand, within mdtQ at 2226.097 kb on + strand, within mdtQ at 2226.118 kb on + strand, within mdtQ at 2226.126 kb on - strand, within mdtQ at 2226.295 kb on - strand, within mdtQ at 2226.310 kb on + strand, within mdtQ at 2226.368 kb on + strand, within mdtQ at 2226.378 kb on - strand, within mdtQ at 2226.398 kb on - strand, within mdtQ at 2226.528 kb on + strand, within mdtQ at 2226.528 kb on + strand, within mdtQ at 2226.668 kb on - strand at 2226.721 kb on + strand at 2226.738 kb on + strand at 2226.753 kb on + strand at 2226.753 kb on + strand at 2226.782 kb on + strand at 2226.782 kb on + strand at 2226.794 kb on + strand at 2226.903 kb on + strand at 2226.934 kb on + strand at 2226.934 kb on + strand at 2226.942 kb on - strand at 2227.010 kb on + strand at 2227.020 kb on + strand, within yohP at 2227.020 kb on + strand, within yohP at 2227.047 kb on + strand, within yohP at 2227.047 kb on + strand, within yohP at 2227.050 kb on + strand, within yohP at 2227.051 kb on + strand, within yohP at 2227.052 kb on + strand, within yohP at 2227.052 kb on + strand, within yohP at 2227.052 kb on + strand, within yohP at 2227.126 kb on + strand at 2227.158 kb on - strand at 2227.158 kb on - strand at 2227.163 kb on - strand at 2227.167 kb on + strand at 2227.168 kb on - strand at 2227.175 kb on - strand at 2227.179 kb on - strand at 2227.179 kb on - strand at 2227.336 kb on + strand at 2227.336 kb on + strand at 2227.345 kb on + strand at 2227.355 kb on + strand at 2227.432 kb on + strand at 2227.592 kb on - strand, within dusC at 2227.617 kb on - strand, within dusC at 2227.620 kb on - strand, within dusC at 2227.620 kb on - strand, within dusC at 2227.663 kb on + strand, within dusC at 2227.663 kb on + strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.671 kb on - strand, within dusC at 2227.674 kb on + strand, within dusC at 2227.675 kb on - strand, within dusC at 2227.676 kb on - strand, within dusC at 2227.747 kb on - strand, within dusC at 2227.752 kb on - strand, within dusC at 2227.752 kb on - strand, within dusC at 2227.767 kb on + strand, within dusC at 2227.767 kb on + strand, within dusC
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 2,224,348 - -1.3 2,224,358 + +0.7 2,224,417 - +1.1 2,224,529 - -0.2 2,224,541 - +1.8 2,224,578 + +0.5 2,224,713 - yohF b2137 0.24 -0.2 2,224,872 - yohF b2137 0.45 -0.2 2,224,902 + yohF b2137 0.49 -1.7 2,224,902 + yohF b2137 0.49 +0.2 2,224,942 + yohF b2137 0.54 +1.3 2,224,992 - yohF b2137 0.60 -1.1 2,224,992 - yohF b2137 0.60 -1.4 2,224,994 - yohF b2137 0.61 -0.0 2,224,994 - yohF b2137 0.61 +0.7 2,225,063 - yohF b2137 0.70 +0.5 2,225,103 - yohF b2137 0.75 -2.8 2,225,133 + yohF b2137 0.79 +0.7 2,225,163 + yohF b2137 0.83 -0.2 2,225,199 - yohF b2137 0.88 -1.4 2,225,292 - +1.2 2,225,409 + +1.2 2,225,429 - +0.3 2,225,476 - +0.2 2,225,538 + mdtQ b2139 0.13 +0.8 2,225,672 - mdtQ b2139 0.23 -0.3 2,225,711 + mdtQ b2139 0.25 -2.4 2,225,719 - mdtQ b2139 0.26 +0.4 2,225,756 - mdtQ b2139 0.29 +2.7 2,225,756 - mdtQ b2139 0.29 +0.1 2,225,777 + mdtQ b2139 0.30 -1.3 2,225,795 + mdtQ b2139 0.31 +0.5 2,225,795 + mdtQ b2139 0.31 +0.7 2,225,843 + mdtQ b2139 0.35 -1.4 2,225,862 + mdtQ b2139 0.36 -0.7 2,225,862 + mdtQ b2139 0.36 -0.1 2,225,867 - mdtQ b2139 0.36 -2.5 2,225,899 + mdtQ b2139 0.39 +0.5 2,225,899 + mdtQ b2139 0.39 +1.9 2,225,906 + mdtQ b2139 0.39 -1.6 2,225,917 - mdtQ b2139 0.40 +1.5 2,225,964 - mdtQ b2139 0.43 +1.3 2,225,964 - mdtQ b2139 0.43 +0.5 2,225,970 + mdtQ b2139 0.44 +0.7 2,226,046 - mdtQ b2139 0.49 +0.5 2,226,046 - mdtQ b2139 0.49 +0.6 2,226,097 + mdtQ b2139 0.52 -0.1 2,226,097 + mdtQ b2139 0.52 -1.5 2,226,118 + mdtQ b2139 0.54 -0.6 2,226,126 - mdtQ b2139 0.54 +1.0 2,226,295 - mdtQ b2139 0.66 +0.5 2,226,310 + mdtQ b2139 0.67 +1.8 2,226,368 + mdtQ b2139 0.71 +0.1 2,226,378 - mdtQ b2139 0.72 +0.5 2,226,398 - mdtQ b2139 0.73 +0.4 2,226,528 + mdtQ b2139 0.82 -0.3 2,226,528 + mdtQ b2139 0.82 +1.7 2,226,668 - -0.7 2,226,721 + -0.3 2,226,738 + +1.0 2,226,753 + -0.1 2,226,753 + -0.6 2,226,782 + +0.7 2,226,782 + -0.3 2,226,794 + +0.3 2,226,903 + -0.4 2,226,934 + -1.3 2,226,934 + +2.7 2,226,942 - +0.7 2,227,010 + -0.6 2,227,020 + yohP b4679 0.19 -0.3 2,227,020 + yohP b4679 0.19 -0.3 2,227,047 + yohP b4679 0.51 +2.3 2,227,047 + yohP b4679 0.51 +2.7 2,227,050 + yohP b4679 0.55 -0.2 2,227,051 + yohP b4679 0.56 +0.7 2,227,052 + yohP b4679 0.57 -1.9 2,227,052 + yohP b4679 0.57 +1.3 2,227,052 + yohP b4679 0.57 +1.5 2,227,126 + +0.7 2,227,158 - +0.7 2,227,158 - +1.7 2,227,163 - +0.4 2,227,167 + -0.5 2,227,168 - +0.7 2,227,175 - -0.3 2,227,179 - +0.7 2,227,179 - +0.7 2,227,336 + -0.3 2,227,336 + -0.2 2,227,345 + -1.6 2,227,355 + -0.3 2,227,432 + +0.7 2,227,592 - dusC b2140 0.14 -1.3 2,227,617 - dusC b2140 0.17 +0.4 2,227,620 - dusC b2140 0.17 -1.0 2,227,620 - dusC b2140 0.17 +0.0 2,227,663 + dusC b2140 0.21 +0.7 2,227,663 + dusC b2140 0.21 +0.7 2,227,671 - dusC b2140 0.22 -0.0 2,227,671 - dusC b2140 0.22 +0.1 2,227,671 - dusC b2140 0.22 -0.1 2,227,671 - dusC b2140 0.22 +0.8 2,227,671 - dusC b2140 0.22 +0.7 2,227,674 + dusC b2140 0.23 +0.7 2,227,675 - dusC b2140 0.23 +0.1 2,227,676 - dusC b2140 0.23 -1.5 2,227,747 - dusC b2140 0.30 -0.9 2,227,752 - dusC b2140 0.31 -2.1 2,227,752 - dusC b2140 0.31 -1.3 2,227,767 + dusC b2140 0.32 -0.9 2,227,767 + dusC b2140 0.32 +0.7
Or see this region's nucleotide sequence