Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yegZ and b2085 are separated by 174 nucleotides b2085 and yegS are separated by 405 nucleotides yegS and gatR are separated by 81 nucleotides gatR and insE are separated by 68 nucleotides insE and insF-5 overlap by 4 nucleotides
b2083: yegZ - orf, hypothetical protein (VIMSS), at 2,165,626 to 2,165,838
yegZ
b2085: b2085 - orf, hypothetical protein (VIMSS), at 2,166,013 to 2,166,330
b2085
b2086: yegS - hypothetical protein (NCBI), at 2,166,736 to 2,167,635
yegS
b4498: gatR - no description, at 2,167,717 to 2,168,191
gatR
b2088: insE - IS3 element protein InsE (RefSeq), at 2,168,260 to 2,168,559
insE
b2089: insF-5 - IS3 element protein InsF (NCBI), at 2,168,556 to 2,169,422
insF-5
Position (kb)
2166
2167
2168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5
6 at 2165.872 kb on + strand at 2165.894 kb on - strand at 2165.907 kb on + strand at 2165.929 kb on + strand at 2165.929 kb on + strand at 2165.964 kb on + strand at 2165.968 kb on + strand at 2166.018 kb on + strand at 2166.018 kb on + strand at 2166.023 kb on + strand at 2166.047 kb on + strand, within b2085 at 2166.047 kb on + strand, within b2085 at 2166.047 kb on - strand, within b2085 at 2166.047 kb on - strand, within b2085 at 2166.052 kb on - strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.054 kb on + strand, within b2085 at 2166.055 kb on + strand, within b2085 at 2166.062 kb on - strand, within b2085 at 2166.102 kb on + strand, within b2085 at 2166.102 kb on + strand, within b2085 at 2166.155 kb on + strand, within b2085 at 2166.188 kb on + strand, within b2085 at 2166.188 kb on + strand, within b2085 at 2166.588 kb on - strand at 2166.634 kb on - strand at 2166.634 kb on - strand at 2166.635 kb on - strand at 2166.636 kb on - strand at 2166.661 kb on - strand at 2166.693 kb on + strand at 2166.693 kb on + strand at 2166.698 kb on + strand at 2166.708 kb on - strand at 2166.713 kb on - strand at 2166.723 kb on - strand at 2166.839 kb on + strand, within yegS at 2166.846 kb on - strand, within yegS at 2166.846 kb on - strand, within yegS at 2166.850 kb on - strand, within yegS at 2166.889 kb on - strand, within yegS at 2166.889 kb on - strand, within yegS at 2166.889 kb on - strand, within yegS at 2166.889 kb on - strand, within yegS at 2166.889 kb on - strand, within yegS at 2166.894 kb on - strand, within yegS at 2166.894 kb on - strand, within yegS at 2166.900 kb on + strand, within yegS at 2166.964 kb on - strand, within yegS at 2166.972 kb on + strand, within yegS at 2166.981 kb on + strand, within yegS at 2167.040 kb on + strand, within yegS at 2167.040 kb on + strand, within yegS at 2167.040 kb on + strand, within yegS at 2167.040 kb on + strand, within yegS at 2167.047 kb on - strand, within yegS at 2167.047 kb on - strand, within yegS at 2167.048 kb on - strand, within yegS at 2167.048 kb on - strand, within yegS at 2167.049 kb on + strand, within yegS at 2167.160 kb on + strand, within yegS at 2167.170 kb on + strand, within yegS at 2167.170 kb on + strand, within yegS at 2167.220 kb on - strand, within yegS at 2167.220 kb on - strand, within yegS at 2167.221 kb on + strand, within yegS at 2167.250 kb on + strand, within yegS at 2167.256 kb on + strand, within yegS at 2167.260 kb on + strand, within yegS at 2167.260 kb on - strand, within yegS at 2167.264 kb on - strand, within yegS at 2167.299 kb on - strand, within yegS at 2167.306 kb on + strand, within yegS at 2167.306 kb on + strand, within yegS at 2167.306 kb on + strand, within yegS at 2167.324 kb on + strand, within yegS at 2167.325 kb on + strand, within yegS at 2167.325 kb on + strand, within yegS at 2167.328 kb on - strand, within yegS at 2167.339 kb on + strand, within yegS at 2167.344 kb on + strand, within yegS at 2167.394 kb on + strand, within yegS at 2167.437 kb on - strand, within yegS at 2167.446 kb on - strand, within yegS at 2167.457 kb on - strand, within yegS at 2167.500 kb on - strand, within yegS at 2167.508 kb on - strand, within yegS at 2167.529 kb on + strand, within yegS at 2167.529 kb on + strand, within yegS at 2167.529 kb on + strand, within yegS at 2167.530 kb on + strand, within yegS at 2167.530 kb on + strand, within yegS at 2167.609 kb on + strand at 2167.616 kb on + strand at 2167.617 kb on + strand at 2167.617 kb on + strand at 2167.619 kb on + strand at 2167.624 kb on + strand at 2167.632 kb on - strand at 2167.643 kb on + strand at 2167.656 kb on - strand at 2167.665 kb on + strand at 2167.714 kb on + strand at 2167.750 kb on - strand at 2167.873 kb on - strand, within gatR at 2167.891 kb on - strand, within gatR at 2167.891 kb on - strand, within gatR at 2167.891 kb on - strand, within gatR at 2167.898 kb on - strand, within gatR at 2167.898 kb on - strand, within gatR at 2167.952 kb on - strand, within gatR at 2167.952 kb on - strand, within gatR at 2167.956 kb on - strand, within gatR at 2167.961 kb on - strand, within gatR at 2167.961 kb on - strand, within gatR at 2167.961 kb on - strand, within gatR at 2167.977 kb on - strand, within gatR at 2168.010 kb on - strand, within gatR at 2168.010 kb on - strand, within gatR at 2168.010 kb on - strand, within gatR at 2168.018 kb on - strand, within gatR at 2168.039 kb on - strand, within gatR at 2168.048 kb on - strand, within gatR at 2168.048 kb on - strand, within gatR at 2168.049 kb on - strand, within gatR at 2168.049 kb on - strand, within gatR at 2168.051 kb on - strand, within gatR at 2168.053 kb on - strand, within gatR at 2168.054 kb on - strand, within gatR at 2168.061 kb on - strand, within gatR at 2168.061 kb on - strand, within gatR at 2168.110 kb on + strand, within gatR at 2168.111 kb on - strand, within gatR at 2168.136 kb on - strand, within gatR at 2168.136 kb on - strand, within gatR at 2168.161 kb on - strand at 2168.161 kb on - strand at 2168.171 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 2,165,872 + -0.5 2,165,894 - -0.5 2,165,907 + +0.2 2,165,929 + +1.2 2,165,929 + +0.3 2,165,964 + +0.1 2,165,968 + -0.3 2,166,018 + +1.2 2,166,018 + -0.3 2,166,023 + +0.7 2,166,047 + b2085 b2085 0.11 -0.3 2,166,047 + b2085 b2085 0.11 -0.3 2,166,047 - b2085 b2085 0.11 -0.3 2,166,047 - b2085 b2085 0.11 -0.3 2,166,052 - b2085 b2085 0.12 +0.7 2,166,054 + b2085 b2085 0.13 -1.2 2,166,054 + b2085 b2085 0.13 +1.7 2,166,054 + b2085 b2085 0.13 +1.0 2,166,054 + b2085 b2085 0.13 +0.0 2,166,054 + b2085 b2085 0.13 -0.0 2,166,054 + b2085 b2085 0.13 +0.2 2,166,055 + b2085 b2085 0.13 +0.7 2,166,062 - b2085 b2085 0.15 -0.3 2,166,102 + b2085 b2085 0.28 +2.2 2,166,102 + b2085 b2085 0.28 +3.1 2,166,155 + b2085 b2085 0.45 -1.3 2,166,188 + b2085 b2085 0.55 +0.7 2,166,188 + b2085 b2085 0.55 +0.7 2,166,588 - +0.7 2,166,634 - -0.3 2,166,634 - -0.3 2,166,635 - -0.3 2,166,636 - -0.3 2,166,661 - +0.7 2,166,693 + -0.3 2,166,693 + +0.7 2,166,698 + +0.7 2,166,708 - +0.1 2,166,713 - +0.7 2,166,723 - +0.8 2,166,839 + yegS b2086 0.11 -0.0 2,166,846 - yegS b2086 0.12 +0.7 2,166,846 - yegS b2086 0.12 +2.7 2,166,850 - yegS b2086 0.13 +2.7 2,166,889 - yegS b2086 0.17 +0.7 2,166,889 - yegS b2086 0.17 +0.7 2,166,889 - yegS b2086 0.17 -1.3 2,166,889 - yegS b2086 0.17 +2.5 2,166,889 - yegS b2086 0.17 -1.3 2,166,894 - yegS b2086 0.18 +1.7 2,166,894 - yegS b2086 0.18 +0.0 2,166,900 + yegS b2086 0.18 -2.1 2,166,964 - yegS b2086 0.25 +0.9 2,166,972 + yegS b2086 0.26 +0.1 2,166,981 + yegS b2086 0.27 +2.7 2,167,040 + yegS b2086 0.34 -0.3 2,167,040 + yegS b2086 0.34 +0.1 2,167,040 + yegS b2086 0.34 -0.9 2,167,040 + yegS b2086 0.34 +0.7 2,167,047 - yegS b2086 0.35 +0.7 2,167,047 - yegS b2086 0.35 -1.9 2,167,048 - yegS b2086 0.35 -0.3 2,167,048 - yegS b2086 0.35 +0.1 2,167,049 + yegS b2086 0.35 -1.7 2,167,160 + yegS b2086 0.47 +1.2 2,167,170 + yegS b2086 0.48 +0.7 2,167,170 + yegS b2086 0.48 +1.0 2,167,220 - yegS b2086 0.54 +3.7 2,167,220 - yegS b2086 0.54 +0.1 2,167,221 + yegS b2086 0.54 -0.1 2,167,250 + yegS b2086 0.57 +0.7 2,167,256 + yegS b2086 0.58 -1.2 2,167,260 + yegS b2086 0.58 +0.5 2,167,260 - yegS b2086 0.58 +0.7 2,167,264 - yegS b2086 0.59 -0.3 2,167,299 - yegS b2086 0.63 +2.2 2,167,306 + yegS b2086 0.63 +1.4 2,167,306 + yegS b2086 0.63 -1.7 2,167,306 + yegS b2086 0.63 +0.7 2,167,324 + yegS b2086 0.65 -0.1 2,167,325 + yegS b2086 0.65 +0.1 2,167,325 + yegS b2086 0.65 -2.3 2,167,328 - yegS b2086 0.66 +0.7 2,167,339 + yegS b2086 0.67 +0.7 2,167,344 + yegS b2086 0.68 +6.3 2,167,394 + yegS b2086 0.73 +0.8 2,167,437 - yegS b2086 0.78 +0.7 2,167,446 - yegS b2086 0.79 -0.9 2,167,457 - yegS b2086 0.80 +0.1 2,167,500 - yegS b2086 0.85 +0.7 2,167,508 - yegS b2086 0.86 -0.9 2,167,529 + yegS b2086 0.88 +0.7 2,167,529 + yegS b2086 0.88 -0.3 2,167,529 + yegS b2086 0.88 -0.3 2,167,530 + yegS b2086 0.88 -1.3 2,167,530 + yegS b2086 0.88 +2.7 2,167,609 + -0.3 2,167,616 + -1.7 2,167,617 + -2.8 2,167,617 + +0.7 2,167,619 + +1.7 2,167,624 + +0.3 2,167,632 - +0.7 2,167,643 + -1.9 2,167,656 - +0.7 2,167,665 + +0.7 2,167,714 + +0.7 2,167,750 - -0.2 2,167,873 - gatR b4498 0.33 -0.9 2,167,891 - gatR b4498 0.37 +0.2 2,167,891 - gatR b4498 0.37 +1.2 2,167,891 - gatR b4498 0.37 +2.2 2,167,898 - gatR b4498 0.38 -0.1 2,167,898 - gatR b4498 0.38 -0.3 2,167,952 - gatR b4498 0.49 -0.8 2,167,952 - gatR b4498 0.49 -2.3 2,167,956 - gatR b4498 0.50 -0.2 2,167,961 - gatR b4498 0.51 -0.3 2,167,961 - gatR b4498 0.51 +1.0 2,167,961 - gatR b4498 0.51 -0.6 2,167,977 - gatR b4498 0.55 -0.1 2,168,010 - gatR b4498 0.62 +0.2 2,168,010 - gatR b4498 0.62 -0.2 2,168,010 - gatR b4498 0.62 -0.2 2,168,018 - gatR b4498 0.63 -0.5 2,168,039 - gatR b4498 0.68 +1.0 2,168,048 - gatR b4498 0.70 +0.3 2,168,048 - gatR b4498 0.70 +1.8 2,168,049 - gatR b4498 0.70 -1.3 2,168,049 - gatR b4498 0.70 -0.3 2,168,051 - gatR b4498 0.70 +0.3 2,168,053 - gatR b4498 0.71 +0.4 2,168,054 - gatR b4498 0.71 -1.8 2,168,061 - gatR b4498 0.72 +1.4 2,168,061 - gatR b4498 0.72 -1.2 2,168,110 + gatR b4498 0.83 +0.7 2,168,111 - gatR b4498 0.83 -1.7 2,168,136 - gatR b4498 0.88 -0.9 2,168,136 - gatR b4498 0.88 -1.7 2,168,161 - -1.9 2,168,161 - -1.3 2,168,171 - -0.9
Or see this region's nucleotide sequence