Strain Fitness in Escherichia coli BW25113 around b1867

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaspS and yecD are separated by 309 nucleotidesyecD and yecE overlap by 4 nucleotidesyecE and yecN are separated by 52 nucleotides b1866: aspS - aspartyl-tRNA synthetase (NCBI), at 1,946,774 to 1,948,546 aspS b1867: yecD - orf, hypothetical protein (VIMSS), at 1,948,856 to 1,949,422 yecD b1868: yecE - hypothetical protein (NCBI), at 1,949,419 to 1,950,237 yecE b1869: yecN - orf, hypothetical protein (VIMSS), at 1,950,290 to 1,950,685 yecN Position (kb) 1948 1949 1950Strain fitness (log2 ratio) -2 -1 0 1 2at 1948.556 kb on - strandat 1948.718 kb on - strandat 1948.761 kb on + strandat 1948.771 kb on + strandat 1948.859 kb on - strandat 1948.859 kb on - strandat 1948.932 kb on + strand, within yecDat 1949.048 kb on + strand, within yecDat 1949.173 kb on + strand, within yecDat 1949.173 kb on + strand, within yecDat 1949.179 kb on - strand, within yecDat 1949.186 kb on + strand, within yecDat 1949.186 kb on + strand, within yecDat 1949.189 kb on - strand, within yecDat 1949.228 kb on + strand, within yecDat 1949.228 kb on + strand, within yecDat 1949.228 kb on - strand, within yecDat 1949.228 kb on - strand, within yecDat 1949.231 kb on - strand, within yecDat 1949.349 kb on + strand, within yecDat 1949.404 kb on - strandat 1949.404 kb on - strandat 1949.442 kb on - strandat 1949.709 kb on + strand, within yecEat 1949.709 kb on + strand, within yecEat 1949.746 kb on - strand, within yecEat 1949.842 kb on - strand, within yecEat 1949.842 kb on - strand, within yecEat 1949.931 kb on + strand, within yecEat 1950.249 kb on + strandat 1950.310 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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1,948,556 - -2.1
1,948,718 - +0.3
1,948,761 + +1.7
1,948,771 + +0.7
1,948,859 - +1.4
1,948,859 - +0.4
1,948,932 + yecD b1867 0.13 +2.2
1,949,048 + yecD b1867 0.34 -0.7
1,949,173 + yecD b1867 0.56 -1.3
1,949,173 + yecD b1867 0.56 -2.1
1,949,179 - yecD b1867 0.57 -0.1
1,949,186 + yecD b1867 0.58 +0.2
1,949,186 + yecD b1867 0.58 -0.8
1,949,189 - yecD b1867 0.59 +1.1
1,949,228 + yecD b1867 0.66 +0.2
1,949,228 + yecD b1867 0.66 +0.4
1,949,228 - yecD b1867 0.66 +0.7
1,949,228 - yecD b1867 0.66 -0.0
1,949,231 - yecD b1867 0.66 +1.0
1,949,349 + yecD b1867 0.87 +0.7
1,949,404 - -2.2
1,949,404 - +0.5
1,949,442 - +0.5
1,949,709 + yecE b1868 0.35 +1.9
1,949,709 + yecE b1868 0.35 -0.7
1,949,746 - yecE b1868 0.40 -0.3
1,949,842 - yecE b1868 0.52 -0.8
1,949,842 - yecE b1868 0.52 -1.0
1,949,931 + yecE b1868 0.63 -1.9
1,950,249 + -2.5
1,950,310 - -0.3

Or see this region's nucleotide sequence