Strain Fitness in Escherichia coli BW25113 around b1605

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydgH and ydgI are separated by 185 nucleotidesydgI and folM are separated by 36 nucleotidesfolM and ydgC overlap by 4 nucleotides b1604: ydgH - hypothetical protein (NCBI), at 1,676,451 to 1,677,395 ydgH b1605: ydgI - predicted arginine/ornithine antiporter transporter (NCBI), at 1,677,581 to 1,678,963 ydgI b1606: folM - short chain dehydrogenase (NCBI), at 1,679,000 to 1,679,722 folM b1607: ydgC - conserved inner membrane protein associated with alginate biosynthesis (NCBI), at 1,679,719 to 1,680,054 ydgC Position (kb) 1677 1678 1679Strain fitness (log2 ratio) -2 -1 0 1 2 3 4 5at 1676.770 kb on + strand, within ydgHat 1676.818 kb on - strand, within ydgHat 1676.938 kb on + strand, within ydgHat 1676.953 kb on + strand, within ydgHat 1677.045 kb on - strand, within ydgHat 1677.184 kb on - strand, within ydgHat 1677.329 kb on - strandat 1677.329 kb on - strandat 1677.791 kb on + strand, within ydgIat 1677.952 kb on + strand, within ydgIat 1678.025 kb on + strand, within ydgIat 1678.025 kb on + strand, within ydgIat 1678.025 kb on + strand, within ydgIat 1678.148 kb on + strand, within ydgIat 1678.154 kb on - strand, within ydgIat 1678.295 kb on - strand, within ydgIat 1678.353 kb on + strand, within ydgIat 1678.353 kb on + strand, within ydgIat 1678.422 kb on - strand, within ydgIat 1678.477 kb on - strand, within ydgIat 1678.873 kb on - strandat 1678.883 kb on + strandat 1678.883 kb on + strandat 1678.919 kb on + strandat 1679.076 kb on + strand, within folMat 1679.076 kb on + strand, within folMat 1679.126 kb on + strand, within folMat 1679.126 kb on + strand, within folMat 1679.165 kb on - strand, within folMat 1679.165 kb on - strand, within folMat 1679.198 kb on - strand, within folMat 1679.226 kb on + strand, within folMat 1679.328 kb on - strand, within folMat 1679.336 kb on - strand, within folMat 1679.342 kb on + strand, within folMat 1679.342 kb on - strand, within folMat 1679.342 kb on - strand, within folMat 1679.342 kb on - strand, within folMat 1679.349 kb on + strand, within folMat 1679.376 kb on + strand, within folMat 1679.390 kb on + strand, within folMat 1679.484 kb on - strand, within folMat 1679.538 kb on + strand, within folMat 1679.538 kb on + strand, within folMat 1679.887 kb on + strand, within ydgCat 1679.897 kb on + strand, within ydgCat 1679.924 kb on - strand, within ydgC

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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1,676,770 + ydgH b1604 0.34 +3.6
1,676,818 - ydgH b1604 0.39 +4.5
1,676,938 + ydgH b1604 0.52 +3.7
1,676,953 + ydgH b1604 0.53 +5.3
1,677,045 - ydgH b1604 0.63 +4.4
1,677,184 - ydgH b1604 0.78 +4.2
1,677,329 - +5.3
1,677,329 - +4.4
1,677,791 + ydgI b1605 0.15 +0.6
1,677,952 + ydgI b1605 0.27 -0.2
1,678,025 + ydgI b1605 0.32 -1.5
1,678,025 + ydgI b1605 0.32 -1.1
1,678,025 + ydgI b1605 0.32 -1.7
1,678,148 + ydgI b1605 0.41 +0.2
1,678,154 - ydgI b1605 0.41 -0.3
1,678,295 - ydgI b1605 0.52 +0.1
1,678,353 + ydgI b1605 0.56 -0.6
1,678,353 + ydgI b1605 0.56 -2.6
1,678,422 - ydgI b1605 0.61 -2.0
1,678,477 - ydgI b1605 0.65 -0.7
1,678,873 - -1.5
1,678,883 + +0.9
1,678,883 + -2.6
1,678,919 + -1.2
1,679,076 + folM b1606 0.11 -1.2
1,679,076 + folM b1606 0.11 -0.7
1,679,126 + folM b1606 0.17 -1.7
1,679,126 + folM b1606 0.17 +2.1
1,679,165 - folM b1606 0.23 -0.0
1,679,165 - folM b1606 0.23 -1.5
1,679,198 - folM b1606 0.27 -0.1
1,679,226 + folM b1606 0.31 +0.2
1,679,328 - folM b1606 0.45 -1.6
1,679,336 - folM b1606 0.46 -1.4
1,679,342 + folM b1606 0.47 +0.4
1,679,342 - folM b1606 0.47 -1.3
1,679,342 - folM b1606 0.47 -2.7
1,679,342 - folM b1606 0.47 +1.4
1,679,349 + folM b1606 0.48 -0.7
1,679,376 + folM b1606 0.52 +0.4
1,679,390 + folM b1606 0.54 -0.9
1,679,484 - folM b1606 0.67 +1.4
1,679,538 + folM b1606 0.74 +0.6
1,679,538 + folM b1606 0.74 +1.1
1,679,887 + ydgC b1607 0.50 -1.7
1,679,897 + ydgC b1607 0.53 +2.5
1,679,924 - ydgC b1607 0.61 +0.9

Or see this region's nucleotide sequence