Strain Fitness in Escherichia coli BW25113 around b1301

Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntpuuC and puuB are separated by 1 nucleotidespuuB and puuE are separated by 37 nucleotides b1300: puuC - gamma-Glu-gamma-aminobutyraldehyde dehydrogenase, NAD(P)H-dependent (NCBI), at 1,360,767 to 1,362,254 puuC b1301: puuB - gamma-Glu-putrescine oxidase, FAD/NAD(P)-binding (NCBI), at 1,362,256 to 1,363,536 puuB b1302: puuE - GABA aminotransferase, PLP-dependent (NCBI), at 1,363,574 to 1,364,839 puuE Position (kb) 1362 1363 1364Strain fitness (log2 ratio) -2 -1 0 1at 1361.262 kb on - strand, within puuCat 1361.563 kb on - strand, within puuCat 1361.674 kb on - strand, within puuCat 1361.727 kb on - strand, within puuCat 1362.160 kb on + strandat 1362.229 kb on - strandat 1362.317 kb on + strandat 1362.520 kb on + strand, within puuBat 1362.664 kb on + strand, within puuBat 1362.716 kb on - strand, within puuBat 1362.757 kb on + strand, within puuBat 1363.276 kb on + strand, within puuBat 1363.284 kb on - strand, within puuBat 1363.311 kb on + strand, within puuBat 1363.311 kb on + strand, within puuBat 1363.332 kb on - strand, within puuBat 1363.341 kb on - strand, within puuBat 1363.503 kb on + strandat 1363.631 kb on - strandat 1363.749 kb on - strand, within puuEat 1363.749 kb on - strand, within puuEat 1363.940 kb on - strand, within puuEat 1363.993 kb on + strand, within puuEat 1364.468 kb on + strand, within puuE

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM
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1,361,262 - puuC b1300 0.33 +0.2
1,361,563 - puuC b1300 0.53 -0.4
1,361,674 - puuC b1300 0.61 +0.4
1,361,727 - puuC b1300 0.65 -0.1
1,362,160 + +0.4
1,362,229 - +0.2
1,362,317 + -0.3
1,362,520 + puuB b1301 0.21 -1.0
1,362,664 + puuB b1301 0.32 -1.1
1,362,716 - puuB b1301 0.36 +1.7
1,362,757 + puuB b1301 0.39 +0.2
1,363,276 + puuB b1301 0.80 -1.3
1,363,284 - puuB b1301 0.80 -2.2
1,363,311 + puuB b1301 0.82 +0.1
1,363,311 + puuB b1301 0.82 -1.3
1,363,332 - puuB b1301 0.84 -0.9
1,363,341 - puuB b1301 0.85 -1.0
1,363,503 + +0.9
1,363,631 - -0.0
1,363,749 - puuE b1302 0.14 -0.3
1,363,749 - puuE b1302 0.14 -0.8
1,363,940 - puuE b1302 0.29 -0.0
1,363,993 + puuE b1302 0.33 +0.9
1,364,468 + puuE b1302 0.71 -0.0

Or see this region's nucleotide sequence