Experiment: LB Anaerobic with Cephalothin 0.0000078125 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt pepN and ssuB are separated by 42 nucleotides ssuB and ycbM overlap by 4 nucleotides ycbM and ssuD are separated by 10 nucleotides
b0932: pepN - aminopeptidase N (NCBI), at 989,845 to 992,457
pepN
b0933: ssuB - alkanesulfonate transporter subunit (NCBI), at 992,500 to 993,267
ssuB
b0934: ycbM - putative transport system permease protein (VIMSS), at 993,264 to 994,055
ycbM
b0935: ssuD - alkanesulfonate monooxygenase (NCBI), at 994,066 to 995,211
ssuD
Position (kb) 993
994
995 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 992.402 kb on - strand at 992.438 kb on + strand at 992.582 kb on - strand, within ssuB at 992.676 kb on + strand, within ssuB at 992.681 kb on + strand, within ssuB at 992.712 kb on + strand, within ssuB at 992.928 kb on - strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.003 kb on + strand, within ssuB at 993.056 kb on - strand, within ssuB at 993.078 kb on - strand, within ssuB at 993.103 kb on + strand, within ssuB at 993.239 kb on - strand at 993.526 kb on + strand, within ycbM at 993.729 kb on - strand, within ycbM at 993.913 kb on + strand, within ycbM at 994.024 kb on + strand at 994.033 kb on - strand at 994.089 kb on + strand at 994.089 kb on + strand at 994.335 kb on - strand, within ssuD at 994.356 kb on + strand, within ssuD at 994.364 kb on - strand, within ssuD at 994.597 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.710 kb on - strand, within ssuD at 994.723 kb on + strand, within ssuD at 994.869 kb on + strand, within ssuD at 994.869 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.900 kb on - strand, within ssuD at 994.962 kb on + strand, within ssuD at 995.043 kb on - strand, within ssuD
Per-strain Table
Position Strand Gene LocusTag Fraction LB Anaerobic with Cephalothin 0.0000078125 mM remove 992,402 - -1.6 992,438 + -0.3 992,582 - ssuB b0933 0.11 -3.1 992,676 + ssuB b0933 0.23 +0.5 992,681 + ssuB b0933 0.24 -0.3 992,712 + ssuB b0933 0.28 -0.5 992,928 - ssuB b0933 0.56 +1.0 993,003 + ssuB b0933 0.65 +0.3 993,003 + ssuB b0933 0.65 +0.7 993,056 - ssuB b0933 0.72 +0.4 993,078 - ssuB b0933 0.75 +2.9 993,103 + ssuB b0933 0.79 +1.7 993,239 - +0.3 993,526 + ycbM b0934 0.33 -0.5 993,729 - ycbM b0934 0.59 +0.9 993,913 + ycbM b0934 0.82 -0.8 994,024 + -1.0 994,033 - -0.1 994,089 + +1.4 994,089 + -0.3 994,335 - ssuD b0935 0.23 -0.3 994,356 + ssuD b0935 0.25 +0.3 994,364 - ssuD b0935 0.26 -0.1 994,597 - ssuD b0935 0.46 -0.3 994,710 - ssuD b0935 0.56 -1.7 994,710 - ssuD b0935 0.56 +0.0 994,723 + ssuD b0935 0.57 +1.1 994,869 + ssuD b0935 0.70 -1.7 994,869 - ssuD b0935 0.70 +1.0 994,900 - ssuD b0935 0.73 +4.1 994,900 - ssuD b0935 0.73 +1.4 994,962 + ssuD b0935 0.78 -0.4 995,043 - ssuD b0935 0.85 +1.0
Or see this region's nucleotide sequence