Strain Fitness in Escherichia coli BW25113 around b1310

Experiment: LB Anaerobic with Trimethoprim 0.00000125 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycjM and ycjN are separated by 13 nucleotidesycjN and ycjO are separated by 20 nucleotidesycjO and ycjP overlap by 14 nucleotides b1309: ycjM - putative polysaccharide hydrolase (VIMSS), at 1,368,240 to 1,369,919 ycjM b1310: ycjN - predicted sugar transporter subunit: periplasmic-binding component of ABC superfamily (NCBI), at 1,369,933 to 1,371,225 ycjN b1311: ycjO - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 1,371,246 to 1,372,127 ycjO b1312: ycjP - predicted sugar transporter subunit: membrane component of ABC superfamily (NCBI), at 1,372,114 to 1,372,956 ycjP Position (kb) 1369 1370 1371 1372Strain fitness (log2 ratio) -2 -1 0 1 2at 1369.030 kb on - strand, within ycjMat 1369.124 kb on + strand, within ycjMat 1369.148 kb on - strand, within ycjMat 1369.164 kb on - strand, within ycjMat 1369.173 kb on + strand, within ycjMat 1369.317 kb on + strand, within ycjMat 1369.317 kb on + strand, within ycjMat 1369.365 kb on + strand, within ycjMat 1369.375 kb on + strand, within ycjMat 1369.414 kb on + strand, within ycjMat 1369.424 kb on + strand, within ycjMat 1369.451 kb on + strand, within ycjMat 1369.451 kb on + strand, within ycjMat 1369.489 kb on + strand, within ycjMat 1369.899 kb on - strandat 1369.976 kb on - strandat 1369.992 kb on - strandat 1370.063 kb on - strand, within ycjNat 1370.064 kb on - strand, within ycjNat 1370.064 kb on - strand, within ycjNat 1370.117 kb on - strand, within ycjNat 1370.173 kb on - strand, within ycjNat 1370.269 kb on + strand, within ycjNat 1370.502 kb on + strand, within ycjNat 1370.527 kb on + strand, within ycjNat 1370.739 kb on - strand, within ycjNat 1370.740 kb on - strand, within ycjNat 1370.811 kb on + strand, within ycjNat 1370.899 kb on - strand, within ycjNat 1370.899 kb on - strand, within ycjNat 1370.985 kb on - strand, within ycjNat 1371.017 kb on + strand, within ycjNat 1371.153 kb on + strandat 1371.153 kb on + strandat 1371.161 kb on - strandat 1371.285 kb on + strandat 1371.323 kb on - strandat 1371.474 kb on + strand, within ycjOat 1371.474 kb on + strand, within ycjOat 1371.508 kb on - strand, within ycjOat 1371.587 kb on + strand, within ycjOat 1371.597 kb on + strand, within ycjOat 1371.652 kb on - strand, within ycjOat 1371.665 kb on - strand, within ycjOat 1371.738 kb on - strand, within ycjOat 1371.738 kb on - strand, within ycjOat 1371.778 kb on - strand, within ycjOat 1371.778 kb on - strand, within ycjOat 1371.938 kb on - strand, within ycjOat 1371.991 kb on + strand, within ycjOat 1372.224 kb on - strand, within ycjP

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Per-strain Table

Position Strand Gene LocusTag Fraction LB Anaerobic with Trimethoprim 0.00000125 mM
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1,369,030 - ycjM b1309 0.47 +1.7
1,369,124 + ycjM b1309 0.53 -0.3
1,369,148 - ycjM b1309 0.54 -0.3
1,369,164 - ycjM b1309 0.55 -0.9
1,369,173 + ycjM b1309 0.56 -0.3
1,369,317 + ycjM b1309 0.64 +1.3
1,369,317 + ycjM b1309 0.64 +2.7
1,369,365 + ycjM b1309 0.67 -2.1
1,369,375 + ycjM b1309 0.68 +0.7
1,369,414 + ycjM b1309 0.70 +0.7
1,369,424 + ycjM b1309 0.70 +0.7
1,369,451 + ycjM b1309 0.72 -0.3
1,369,451 + ycjM b1309 0.72 +0.7
1,369,489 + ycjM b1309 0.74 +0.7
1,369,899 - +0.7
1,369,976 - +0.2
1,369,992 - -0.3
1,370,063 - ycjN b1310 0.10 +0.7
1,370,064 - ycjN b1310 0.10 +2.3
1,370,064 - ycjN b1310 0.10 +0.4
1,370,117 - ycjN b1310 0.14 +0.7
1,370,173 - ycjN b1310 0.19 +1.3
1,370,269 + ycjN b1310 0.26 +1.9
1,370,502 + ycjN b1310 0.44 -2.1
1,370,527 + ycjN b1310 0.46 +0.5
1,370,739 - ycjN b1310 0.62 -1.7
1,370,740 - ycjN b1310 0.62 +0.5
1,370,811 + ycjN b1310 0.68 +0.3
1,370,899 - ycjN b1310 0.75 +0.7
1,370,899 - ycjN b1310 0.75 +0.1
1,370,985 - ycjN b1310 0.81 +0.9
1,371,017 + ycjN b1310 0.84 +0.1
1,371,153 + +0.1
1,371,153 + -0.1
1,371,161 - +0.4
1,371,285 + -0.4
1,371,323 - -0.3
1,371,474 + ycjO b1311 0.26 +0.5
1,371,474 + ycjO b1311 0.26 +0.4
1,371,508 - ycjO b1311 0.30 -1.3
1,371,587 + ycjO b1311 0.39 +0.3
1,371,597 + ycjO b1311 0.40 +0.1
1,371,652 - ycjO b1311 0.46 +0.0
1,371,665 - ycjO b1311 0.48 +1.7
1,371,738 - ycjO b1311 0.56 +0.5
1,371,738 - ycjO b1311 0.56 -0.6
1,371,778 - ycjO b1311 0.60 -0.8
1,371,778 - ycjO b1311 0.60 -0.5
1,371,938 - ycjO b1311 0.78 -0.4
1,371,991 + ycjO b1311 0.84 +1.1
1,372,224 - ycjP b1312 0.13 +0.0

Or see this region's nucleotide sequence