Experiment: KB with Strychnine Sulfate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_4385 and Psyr_4386 are separated by 330 nucleotides Psyr_4386 and Psyr_4387 are separated by 95 nucleotides Psyr_4387 and Psyr_4388 are separated by 108 nucleotides Psyr_4388 and Psyr_4389 are separated by 42 nucleotides
Psyr_4385: Psyr_4385 - Alpha-2-macroglobulin, N-terminal:Alpha-2-macroglobulin, N-terminal, at 5,242,625 to 5,247,583
_4385
Psyr_4386: Psyr_4386 - conserved hypothetical protein, at 5,247,914 to 5,248,207
_4386
Psyr_4387: Psyr_4387 - Glycosyl transferase, family 51:Penicillin-binding protein, transpeptidase:Penicillin-binding, C-terminal, at 5,248,303 to 5,250,651
_4387
Psyr_4388: Psyr_4388 - Response regulator receiver, at 5,250,760 to 5,251,167
_4388
Psyr_4389: Psyr_4389 - Flp/Fap pilin component, at 5,251,210 to 5,251,416
_4389
Position (kb) 5248
5249
5250
5251 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 5247.411 kb on - strand at 5247.411 kb on - strand at 5247.458 kb on + strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.459 kb on - strand at 5247.521 kb on + strand at 5247.522 kb on - strand at 5247.522 kb on - strand at 5247.612 kb on + strand at 5247.658 kb on - strand at 5247.721 kb on - strand at 5247.760 kb on - strand at 5247.760 kb on - strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.199 kb on + strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.200 kb on - strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.207 kb on + strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.208 kb on - strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.211 kb on + strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.212 kb on - strand at 5248.288 kb on + strand at 5248.288 kb on + strand at 5248.289 kb on - strand at 5248.458 kb on + strand at 5248.532 kb on - strand at 5248.532 kb on - strand at 5248.532 kb on - strand at 5248.534 kb on + strand at 5248.534 kb on + strand at 5248.555 kb on + strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.556 kb on - strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.573 kb on + strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.574 kb on - strand, within Psyr_4387 at 5248.644 kb on - strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.704 kb on + strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.705 kb on - strand, within Psyr_4387 at 5248.787 kb on - strand, within Psyr_4387 at 5248.846 kb on + strand, within Psyr_4387 at 5248.846 kb on + strand, within Psyr_4387 at 5249.254 kb on + strand, within Psyr_4387 at 5249.255 kb on - strand, within Psyr_4387 at 5249.662 kb on + strand, within Psyr_4387 at 5249.663 kb on - strand, within Psyr_4387 at 5249.822 kb on + strand, within Psyr_4387 at 5249.823 kb on - strand, within Psyr_4387 at 5249.823 kb on - strand, within Psyr_4387 at 5250.002 kb on + strand, within Psyr_4387 at 5250.002 kb on + strand, within Psyr_4387 at 5250.002 kb on + strand, within Psyr_4387 at 5250.003 kb on - strand, within Psyr_4387 at 5250.003 kb on - strand, within Psyr_4387 at 5250.003 kb on - strand, within Psyr_4387 at 5250.003 kb on - strand, within Psyr_4387 at 5250.593 kb on + strand at 5250.593 kb on + strand at 5250.593 kb on + strand at 5250.594 kb on - strand at 5250.827 kb on + strand, within Psyr_4388 at 5250.828 kb on - strand, within Psyr_4388 at 5250.828 kb on - strand, within Psyr_4388 at 5250.828 kb on - strand, within Psyr_4388 at 5251.174 kb on - strand at 5251.201 kb on - strand at 5251.201 kb on - strand at 5251.201 kb on - strand at 5251.201 kb on - strand at 5251.203 kb on + strand at 5251.211 kb on + strand at 5251.211 kb on + strand at 5251.211 kb on + strand at 5251.212 kb on - strand at 5251.212 kb on - strand at 5251.212 kb on - strand at 5251.217 kb on - strand at 5251.221 kb on + strand at 5251.221 kb on + strand at 5251.224 kb on + strand at 5251.225 kb on - strand at 5251.245 kb on + strand, within Psyr_4389 at 5251.245 kb on + strand, within Psyr_4389 at 5251.266 kb on + strand, within Psyr_4389 at 5251.267 kb on - strand, within Psyr_4389 at 5251.332 kb on - strand, within Psyr_4389 at 5251.377 kb on + strand, within Psyr_4389 at 5251.378 kb on - strand, within Psyr_4389 at 5251.378 kb on - strand, within Psyr_4389 at 5251.385 kb on + strand, within Psyr_4389 at 5251.385 kb on + strand, within Psyr_4389 at 5251.385 kb on + strand, within Psyr_4389 at 5251.386 kb on - strand, within Psyr_4389 at 5251.386 kb on - strand, within Psyr_4389 at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.397 kb on + strand at 5251.398 kb on - strand at 5251.398 kb on - strand at 5251.398 kb on - strand at 5251.398 kb on - strand at 5251.398 kb on - strand at 5251.450 kb on - strand at 5251.465 kb on + strand at 5251.465 kb on + strand at 5251.465 kb on + strand at 5251.465 kb on + strand at 5251.465 kb on + strand at 5251.466 kb on - strand at 5251.466 kb on - strand at 5251.466 kb on - strand at 5251.466 kb on - strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.467 kb on + strand at 5251.468 kb on - strand at 5251.468 kb on - strand at 5251.468 kb on - strand at 5251.468 kb on - strand at 5251.468 kb on - strand at 5251.468 kb on - strand at 5251.541 kb on + strand at 5251.581 kb on - strand at 5251.607 kb on + strand at 5251.642 kb on + strand at 5251.642 kb on + strand at 5251.643 kb on - strand at 5251.643 kb on - strand at 5251.643 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Strychnine Sulfate 10 mM remove 5,247,411 - +0.1 5,247,411 - +0.1 5,247,458 + +0.3 5,247,459 - -0.4 5,247,459 - -0.2 5,247,459 - -0.1 5,247,521 + -0.0 5,247,522 - -0.9 5,247,522 - -0.8 5,247,612 + -0.9 5,247,658 - -0.3 5,247,721 - -0.5 5,247,760 - +0.5 5,247,760 - +0.4 5,248,199 + -0.9 5,248,199 + +0.6 5,248,199 + +0.8 5,248,199 + +0.6 5,248,199 + -0.1 5,248,199 + +0.5 5,248,200 - +0.7 5,248,200 - +0.3 5,248,200 - +2.9 5,248,207 + +0.2 5,248,207 + +0.1 5,248,207 + +1.5 5,248,207 + -0.6 5,248,207 + +0.1 5,248,207 + +0.3 5,248,207 + -0.2 5,248,207 + -0.9 5,248,207 + +0.8 5,248,207 + -0.6 5,248,207 + -0.1 5,248,207 + +0.2 5,248,207 + +0.0 5,248,207 + +2.3 5,248,207 + -0.5 5,248,207 + +0.5 5,248,207 + -0.3 5,248,207 + -1.5 5,248,208 - +0.0 5,248,208 - +0.3 5,248,208 - +1.6 5,248,208 - +0.4 5,248,208 - -0.4 5,248,208 - -0.2 5,248,208 - +1.3 5,248,208 - +0.2 5,248,208 - -0.1 5,248,208 - +0.2 5,248,208 - -0.4 5,248,208 - -1.1 5,248,208 - +0.3 5,248,208 - -0.5 5,248,208 - +0.8 5,248,208 - -0.1 5,248,211 + -0.1 5,248,211 + +0.7 5,248,211 + +0.3 5,248,211 + -0.1 5,248,211 + -1.0 5,248,211 + +0.4 5,248,211 + +0.4 5,248,211 + +0.0 5,248,211 + +0.3 5,248,211 + +0.1 5,248,211 + +0.2 5,248,211 + +1.3 5,248,211 + +1.2 5,248,211 + -1.3 5,248,211 + -0.5 5,248,211 + +0.8 5,248,211 + +1.9 5,248,211 + -0.5 5,248,211 + -0.4 5,248,211 + -2.0 5,248,211 + -0.7 5,248,212 - -0.4 5,248,212 - -0.3 5,248,212 - -0.7 5,248,212 - +1.3 5,248,212 - +0.4 5,248,212 - -0.1 5,248,212 - +2.6 5,248,212 - +0.9 5,248,212 - -0.3 5,248,212 - +0.0 5,248,212 - -0.3 5,248,212 - +0.3 5,248,212 - +0.2 5,248,212 - +0.5 5,248,212 - -1.5 5,248,212 - -0.2 5,248,212 - -0.5 5,248,212 - +0.5 5,248,212 - +1.3 5,248,212 - -1.7 5,248,212 - +0.1 5,248,212 - +0.3 5,248,212 - +1.4 5,248,212 - -0.2 5,248,212 - -0.3 5,248,212 - +0.1 5,248,212 - -1.1 5,248,212 - +1.0 5,248,288 + -0.6 5,248,288 + -0.0 5,248,289 - +1.1 5,248,458 + -0.2 5,248,532 - +0.1 5,248,532 - -0.2 5,248,532 - -0.8 5,248,534 + -0.1 5,248,534 + +0.7 5,248,555 + Psyr_4387 0.11 -1.0 5,248,556 - Psyr_4387 0.11 +0.3 5,248,556 - Psyr_4387 0.11 +0.5 5,248,573 + Psyr_4387 0.11 -0.3 5,248,573 + Psyr_4387 0.11 +0.6 5,248,573 + Psyr_4387 0.11 +0.1 5,248,574 - Psyr_4387 0.12 +0.4 5,248,574 - Psyr_4387 0.12 +0.1 5,248,574 - Psyr_4387 0.12 -0.7 5,248,644 - Psyr_4387 0.15 +0.2 5,248,704 + Psyr_4387 0.17 -1.2 5,248,704 + Psyr_4387 0.17 +0.2 5,248,704 + Psyr_4387 0.17 +0.4 5,248,704 + Psyr_4387 0.17 -0.4 5,248,704 + Psyr_4387 0.17 +0.1 5,248,704 + Psyr_4387 0.17 -1.3 5,248,704 + Psyr_4387 0.17 -0.4 5,248,705 - Psyr_4387 0.17 +0.5 5,248,705 - Psyr_4387 0.17 +1.0 5,248,705 - Psyr_4387 0.17 -0.7 5,248,705 - Psyr_4387 0.17 +1.6 5,248,705 - Psyr_4387 0.17 -0.2 5,248,705 - Psyr_4387 0.17 +0.5 5,248,787 - Psyr_4387 0.21 +0.2 5,248,846 + Psyr_4387 0.23 +0.7 5,248,846 + Psyr_4387 0.23 +0.6 5,249,254 + Psyr_4387 0.40 -0.2 5,249,255 - Psyr_4387 0.41 -0.0 5,249,662 + Psyr_4387 0.58 +2.0 5,249,663 - Psyr_4387 0.58 +1.6 5,249,822 + Psyr_4387 0.65 -0.2 5,249,823 - Psyr_4387 0.65 -0.5 5,249,823 - Psyr_4387 0.65 -0.8 5,250,002 + Psyr_4387 0.72 +1.1 5,250,002 + Psyr_4387 0.72 -0.4 5,250,002 + Psyr_4387 0.72 -1.0 5,250,003 - Psyr_4387 0.72 +0.1 5,250,003 - Psyr_4387 0.72 -0.2 5,250,003 - Psyr_4387 0.72 -0.6 5,250,003 - Psyr_4387 0.72 -0.5 5,250,593 + -0.5 5,250,593 + +1.5 5,250,593 + -0.9 5,250,594 - -1.0 5,250,827 + Psyr_4388 0.16 +1.3 5,250,828 - Psyr_4388 0.17 +0.2 5,250,828 - Psyr_4388 0.17 -1.0 5,250,828 - Psyr_4388 0.17 +0.1 5,251,174 - +0.1 5,251,201 - -0.5 5,251,201 - +0.5 5,251,201 - +0.4 5,251,201 - +0.6 5,251,203 + -0.6 5,251,211 + -1.7 5,251,211 + +0.7 5,251,211 + -0.6 5,251,212 - +0.3 5,251,212 - -0.3 5,251,212 - -0.2 5,251,217 - +0.4 5,251,221 + -2.7 5,251,221 + +2.3 5,251,224 + +1.3 5,251,225 - +0.3 5,251,245 + Psyr_4389 0.17 +0.5 5,251,245 + Psyr_4389 0.17 -0.3 5,251,266 + Psyr_4389 0.27 -0.7 5,251,267 - Psyr_4389 0.28 -0.1 5,251,332 - Psyr_4389 0.59 -0.7 5,251,377 + Psyr_4389 0.81 -0.3 5,251,378 - Psyr_4389 0.81 +0.1 5,251,378 - Psyr_4389 0.81 +0.4 5,251,385 + Psyr_4389 0.85 -1.0 5,251,385 + Psyr_4389 0.85 -2.5 5,251,385 + Psyr_4389 0.85 +0.3 5,251,386 - Psyr_4389 0.85 +1.3 5,251,386 - Psyr_4389 0.85 -0.6 5,251,397 + -0.7 5,251,397 + -2.6 5,251,397 + -1.4 5,251,397 + -2.8 5,251,397 + +0.1 5,251,397 + -1.3 5,251,397 + -0.6 5,251,398 - +0.3 5,251,398 - -0.3 5,251,398 - +0.5 5,251,398 - -0.7 5,251,398 - -0.7 5,251,450 - -0.3 5,251,465 + -0.0 5,251,465 + -1.7 5,251,465 + -0.3 5,251,465 + -0.9 5,251,465 + -1.6 5,251,466 - -0.4 5,251,466 - +0.6 5,251,466 - -0.3 5,251,466 - +2.3 5,251,467 + -0.7 5,251,467 + -1.6 5,251,467 + -1.5 5,251,467 + -0.1 5,251,467 + -0.1 5,251,467 + -0.3 5,251,467 + -0.9 5,251,467 + +0.6 5,251,467 + -0.6 5,251,467 + +0.1 5,251,468 - -0.6 5,251,468 - -0.8 5,251,468 - -0.1 5,251,468 - -0.4 5,251,468 - +0.4 5,251,468 - +0.4 5,251,541 + -0.8 5,251,581 - +1.2 5,251,607 + -0.8 5,251,642 + +0.3 5,251,642 + +1.2 5,251,643 - -1.0 5,251,643 - +1.5 5,251,643 - -0.1
Or see this region's nucleotide sequence