Experiment: KB with Strychnine Sulfate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_3552 and Psyr_3553 are separated by 325 nucleotides Psyr_3553 and Psyr_RNA54 are separated by 44 nucleotides Psyr_RNA54 and Psyr_RNA55 are separated by 87 nucleotides Psyr_RNA55 and Psyr_RNA56 are separated by 109 nucleotides Psyr_RNA56 and Psyr_3554 are separated by 62 nucleotides Psyr_3554 and Psyr_3555 are separated by 169 nucleotides
Psyr_3552: Psyr_3552 - Divalent cation transporter, at 4,230,613 to 4,232,055
_3552
Psyr_3553: Psyr_3553 - conserved hypothetical protein, at 4,232,381 to 4,232,872
_3553
Psyr_RNA54: Psyr_RNA54 - tRNA-Arg, at 4,232,917 to 4,232,993
_RNA54
Psyr_RNA55: Psyr_RNA55 - tRNA-Arg, at 4,233,081 to 4,233,157
_RNA55
Psyr_RNA56: Psyr_RNA56 - tRNA-Ser, at 4,233,267 to 4,233,357
_RNA56
Psyr_3554: Psyr_3554 - Carbon storage regulator, at 4,233,420 to 4,233,608
_3554
Psyr_3555: Psyr_3555 - aspartate kinase, at 4,233,778 to 4,235,010
_3555
Position (kb) 4232
4233 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 4231.845 kb on - strand, within Psyr_3552 at 4232.056 kb on - strand at 4232.199 kb on - strand at 4232.199 kb on - strand at 4232.199 kb on - strand at 4232.199 kb on - strand at 4232.199 kb on - strand at 4232.344 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.400 kb on + strand at 4232.401 kb on - strand at 4232.401 kb on - strand at 4232.401 kb on - strand at 4232.492 kb on - strand, within Psyr_3553 at 4232.492 kb on - strand, within Psyr_3553 at 4232.596 kb on + strand, within Psyr_3553 at 4232.597 kb on - strand, within Psyr_3553 at 4232.597 kb on - strand, within Psyr_3553 at 4232.795 kb on + strand, within Psyr_3553 at 4232.795 kb on + strand, within Psyr_3553 at 4232.795 kb on + strand, within Psyr_3553 at 4232.795 kb on + strand, within Psyr_3553 at 4232.795 kb on + strand, within Psyr_3553 at 4232.795 kb on + strand, within Psyr_3553 at 4232.796 kb on - strand, within Psyr_3553 at 4232.801 kb on + strand, within Psyr_3553 at 4232.801 kb on + strand, within Psyr_3553 at 4232.801 kb on + strand, within Psyr_3553 at 4232.802 kb on - strand, within Psyr_3553 at 4232.802 kb on - strand, within Psyr_3553 at 4232.827 kb on + strand at 4232.828 kb on - strand at 4232.858 kb on - strand at 4232.870 kb on + strand at 4232.916 kb on - strand at 4232.916 kb on - strand at 4232.916 kb on - strand at 4232.916 kb on - strand at 4232.986 kb on - strand at 4233.050 kb on - strand at 4233.257 kb on - strand at 4233.257 kb on - strand at 4233.456 kb on - strand, within Psyr_3554 at 4233.513 kb on - strand, within Psyr_3554 at 4233.608 kb on + strand at 4233.608 kb on + strand at 4233.653 kb on + strand at 4233.653 kb on + strand at 4233.654 kb on - strand at 4233.654 kb on - strand at 4233.654 kb on - strand at 4233.654 kb on - strand at 4233.654 kb on - strand at 4233.654 kb on - strand at 4233.656 kb on - strand at 4233.694 kb on + strand at 4233.694 kb on + strand at 4233.694 kb on + strand at 4233.694 kb on + strand at 4233.694 kb on + strand at 4233.694 kb on + strand at 4233.694 kb on + strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.695 kb on - strand at 4233.697 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Strychnine Sulfate 10 mM remove 4,231,845 - Psyr_3552 0.85 +0.4 4,232,056 - -0.7 4,232,199 - -1.4 4,232,199 - -0.1 4,232,199 - +1.5 4,232,199 - -0.4 4,232,199 - +1.1 4,232,344 + -0.4 4,232,400 + -1.2 4,232,400 + -0.5 4,232,400 + +0.3 4,232,400 + -0.2 4,232,400 + +0.8 4,232,400 + -0.6 4,232,401 - +0.3 4,232,401 - +0.5 4,232,401 - -0.6 4,232,492 - Psyr_3553 0.23 +1.1 4,232,492 - Psyr_3553 0.23 -1.0 4,232,596 + Psyr_3553 0.44 -0.7 4,232,597 - Psyr_3553 0.44 -0.2 4,232,597 - Psyr_3553 0.44 -0.7 4,232,795 + Psyr_3553 0.84 +3.1 4,232,795 + Psyr_3553 0.84 -0.8 4,232,795 + Psyr_3553 0.84 +1.1 4,232,795 + Psyr_3553 0.84 -0.7 4,232,795 + Psyr_3553 0.84 +0.8 4,232,795 + Psyr_3553 0.84 -0.1 4,232,796 - Psyr_3553 0.84 -0.8 4,232,801 + Psyr_3553 0.85 -0.5 4,232,801 + Psyr_3553 0.85 -1.3 4,232,801 + Psyr_3553 0.85 -0.2 4,232,802 - Psyr_3553 0.86 -0.9 4,232,802 - Psyr_3553 0.86 +0.2 4,232,827 + +0.9 4,232,828 - -2.1 4,232,858 - -0.9 4,232,870 + +0.1 4,232,916 - +1.7 4,232,916 - +1.1 4,232,916 - +0.2 4,232,916 - +1.1 4,232,986 - -1.9 4,233,050 - +0.2 4,233,257 - -0.9 4,233,257 - -0.8 4,233,456 - Psyr_3554 0.19 +1.7 4,233,513 - Psyr_3554 0.49 -2.2 4,233,608 + -1.5 4,233,608 + -0.9 4,233,653 + +0.7 4,233,653 + -0.8 4,233,654 - -0.5 4,233,654 - -0.5 4,233,654 - -0.7 4,233,654 - -0.5 4,233,654 - -1.1 4,233,654 - +0.2 4,233,656 - -0.1 4,233,694 + -1.1 4,233,694 + +0.5 4,233,694 + +0.5 4,233,694 + -1.2 4,233,694 + +1.7 4,233,694 + +2.4 4,233,694 + -0.1 4,233,695 - +0.7 4,233,695 - +1.5 4,233,695 - -0.9 4,233,695 - +0.4 4,233,695 - -0.5 4,233,695 - +0.3 4,233,695 - -1.2 4,233,695 - -1.1 4,233,695 - -0.5 4,233,695 - -0.3 4,233,697 - -0.2
Or see this region's nucleotide sequence