Experiment: KB with Strychnine Sulfate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_2641 and Psyr_2642 overlap by 4 nucleotides Psyr_2642 and Psyr_2643 are separated by 906 nucleotides
Psyr_2641: Psyr_2641 - hypothetical protein, at 3,209,021 to 3,209,428
_2641
Psyr_2642: Psyr_2642 - hypothetical protein, at 3,209,425 to 3,210,069
_2642
Psyr_2643: Psyr_2643 - hypothetical protein, at 3,210,976 to 3,212,205
_2643
Position (kb)
3209
3210
3211 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 3208.491 kb on + strand at 3208.609 kb on - strand at 3208.625 kb on + strand at 3208.625 kb on + strand at 3208.625 kb on + strand at 3208.626 kb on - strand at 3208.626 kb on - strand at 3208.626 kb on - strand at 3208.626 kb on - strand at 3208.741 kb on + strand at 3208.741 kb on + strand at 3208.741 kb on + strand at 3208.742 kb on - strand at 3208.742 kb on - strand at 3208.766 kb on + strand at 3208.863 kb on + strand at 3208.917 kb on + strand at 3208.918 kb on - strand at 3208.918 kb on - strand at 3208.948 kb on + strand at 3208.949 kb on - strand at 3208.990 kb on + strand at 3209.117 kb on + strand, within Psyr_2641 at 3209.118 kb on - strand, within Psyr_2641 at 3209.118 kb on - strand, within Psyr_2641 at 3209.139 kb on + strand, within Psyr_2641 at 3209.216 kb on + strand, within Psyr_2641 at 3209.301 kb on - strand, within Psyr_2641 at 3209.301 kb on - strand, within Psyr_2641 at 3209.301 kb on - strand, within Psyr_2641 at 3209.415 kb on + strand at 3209.448 kb on + strand at 3209.449 kb on - strand at 3209.449 kb on - strand at 3209.543 kb on - strand, within Psyr_2642 at 3209.642 kb on - strand, within Psyr_2642 at 3209.642 kb on - strand, within Psyr_2642 at 3209.642 kb on - strand, within Psyr_2642 at 3209.749 kb on + strand, within Psyr_2642 at 3209.761 kb on - strand, within Psyr_2642 at 3209.774 kb on + strand, within Psyr_2642 at 3209.775 kb on - strand, within Psyr_2642 at 3209.883 kb on + strand, within Psyr_2642 at 3209.884 kb on - strand, within Psyr_2642 at 3209.948 kb on + strand, within Psyr_2642 at 3210.058 kb on + strand at 3210.078 kb on + strand at 3210.094 kb on + strand at 3210.095 kb on - strand at 3210.169 kb on - strand at 3210.204 kb on - strand at 3210.302 kb on + strand at 3210.311 kb on + strand at 3210.342 kb on + strand at 3210.342 kb on + strand at 3210.343 kb on - strand at 3210.559 kb on - strand at 3210.565 kb on - strand at 3210.606 kb on + strand at 3210.607 kb on - strand at 3210.690 kb on + strand at 3210.724 kb on - strand at 3210.810 kb on + strand at 3210.823 kb on - strand at 3210.929 kb on + strand at 3210.952 kb on + strand at 3211.024 kb on + strand at 3211.025 kb on - strand at 3211.025 kb on - strand at 3211.067 kb on - strand at 3211.069 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Strychnine Sulfate 10 mM remove 3,208,491 + +0.2 3,208,609 - -3.0 3,208,625 + +0.9 3,208,625 + -0.5 3,208,625 + -0.2 3,208,626 - -0.8 3,208,626 - -0.5 3,208,626 - -2.0 3,208,626 - -0.6 3,208,741 + -0.3 3,208,741 + -0.9 3,208,741 + -1.8 3,208,742 - -1.0 3,208,742 - +0.6 3,208,766 + -0.5 3,208,863 + +0.7 3,208,917 + -2.0 3,208,918 - +0.6 3,208,918 - +1.0 3,208,948 + -0.5 3,208,949 - -0.7 3,208,990 + +0.5 3,209,117 + Psyr_2641 0.24 -1.0 3,209,118 - Psyr_2641 0.24 -1.0 3,209,118 - Psyr_2641 0.24 -0.4 3,209,139 + Psyr_2641 0.29 -2.0 3,209,216 + Psyr_2641 0.48 -1.8 3,209,301 - Psyr_2641 0.69 -0.8 3,209,301 - Psyr_2641 0.69 -1.1 3,209,301 - Psyr_2641 0.69 -2.1 3,209,415 + -0.6 3,209,448 + -1.3 3,209,449 - +0.6 3,209,449 - +0.9 3,209,543 - Psyr_2642 0.18 -1.6 3,209,642 - Psyr_2642 0.34 -0.5 3,209,642 - Psyr_2642 0.34 -3.1 3,209,642 - Psyr_2642 0.34 -0.7 3,209,749 + Psyr_2642 0.50 -0.8 3,209,761 - Psyr_2642 0.52 -2.8 3,209,774 + Psyr_2642 0.54 -1.8 3,209,775 - Psyr_2642 0.54 -3.3 3,209,883 + Psyr_2642 0.71 -2.7 3,209,884 - Psyr_2642 0.71 +0.5 3,209,948 + Psyr_2642 0.81 -1.0 3,210,058 + +0.0 3,210,078 + -2.4 3,210,094 + -2.3 3,210,095 - -1.5 3,210,169 - -0.6 3,210,204 - +0.5 3,210,302 + -1.4 3,210,311 + +0.0 3,210,342 + -0.4 3,210,342 + -0.7 3,210,343 - -0.2 3,210,559 - -1.0 3,210,565 - -2.0 3,210,606 + -0.1 3,210,607 - -2.0 3,210,690 + -2.2 3,210,724 - -1.6 3,210,810 + -2.9 3,210,823 - +0.3 3,210,929 + +0.4 3,210,952 + -0.0 3,211,024 + -0.8 3,211,025 - -0.8 3,211,025 - -0.6 3,211,067 - -0.9 3,211,069 + +0.0
Or see this region's nucleotide sequence