Experiment: KB with Strychnine Sulfate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_1612 and Psyr_1613 are separated by 231 nucleotides Psyr_1613 and Psyr_1614 are separated by 120 nucleotides
Psyr_1612: Psyr_1612 - septum site-determining protein MinD, at 1,814,844 to 1,815,656
_1612
Psyr_1613: Psyr_1613 - septum site-determining protein MinC, at 1,815,888 to 1,816,628
_1613
Psyr_1614: Psyr_1614 - lipid A biosynthesis acyltransferase, at 1,816,749 to 1,817,681
_1614
Position (kb) 1815
1816
1817 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 1814.944 kb on + strand, within Psyr_1612 at 1815.010 kb on - strand, within Psyr_1612 at 1815.113 kb on - strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.259 kb on + strand, within Psyr_1612 at 1815.260 kb on - strand, within Psyr_1612 at 1815.260 kb on - strand, within Psyr_1612 at 1815.314 kb on - strand, within Psyr_1612 at 1815.764 kb on - strand at 1815.775 kb on - strand at 1815.790 kb on + strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.791 kb on - strand at 1815.889 kb on + strand at 1815.982 kb on + strand, within Psyr_1613 at 1815.982 kb on + strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1815.983 kb on - strand, within Psyr_1613 at 1816.168 kb on + strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.169 kb on - strand, within Psyr_1613 at 1816.473 kb on - strand, within Psyr_1613 at 1816.636 kb on - strand at 1816.637 kb on + strand at 1816.637 kb on + strand at 1816.637 kb on + strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.638 kb on - strand at 1816.671 kb on + strand at 1816.671 kb on + strand at 1816.671 kb on + strand at 1816.671 kb on + strand at 1816.672 kb on - strand at 1816.672 kb on - strand at 1816.674 kb on - strand at 1816.674 kb on - strand at 1816.674 kb on - strand at 1816.766 kb on + strand at 1816.766 kb on + strand at 1816.890 kb on + strand, within Psyr_1614 at 1816.890 kb on + strand, within Psyr_1614 at 1816.890 kb on + strand, within Psyr_1614 at 1816.891 kb on - strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.202 kb on + strand, within Psyr_1614 at 1817.203 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.315 kb on - strand, within Psyr_1614 at 1817.358 kb on + strand, within Psyr_1614 at 1817.525 kb on + strand, within Psyr_1614
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Strychnine Sulfate 10 mM remove 1,814,944 + Psyr_1612 0.12 -1.2 1,815,010 - Psyr_1612 0.20 -0.9 1,815,113 - Psyr_1612 0.33 -0.5 1,815,259 + Psyr_1612 0.51 -0.5 1,815,259 + Psyr_1612 0.51 -1.3 1,815,259 + Psyr_1612 0.51 -1.2 1,815,259 + Psyr_1612 0.51 -1.5 1,815,260 - Psyr_1612 0.51 -1.5 1,815,260 - Psyr_1612 0.51 +0.1 1,815,314 - Psyr_1612 0.58 -0.9 1,815,764 - -0.2 1,815,775 - -0.4 1,815,790 + +0.1 1,815,791 - -0.3 1,815,791 - -0.1 1,815,791 - -0.8 1,815,791 - -1.2 1,815,889 + -0.5 1,815,982 + Psyr_1613 0.13 -1.5 1,815,982 + Psyr_1613 0.13 -2.2 1,815,983 - Psyr_1613 0.13 -2.9 1,815,983 - Psyr_1613 0.13 -1.7 1,816,168 + Psyr_1613 0.38 +0.1 1,816,169 - Psyr_1613 0.38 -1.5 1,816,169 - Psyr_1613 0.38 -1.5 1,816,473 - Psyr_1613 0.79 -1.5 1,816,636 - -1.8 1,816,637 + -0.5 1,816,637 + -2.2 1,816,637 + +1.1 1,816,638 - +0.4 1,816,638 - -0.3 1,816,638 - -0.1 1,816,638 - +0.5 1,816,638 - -1.0 1,816,638 - -0.3 1,816,638 - -0.3 1,816,638 - +1.1 1,816,638 - +0.5 1,816,638 - -1.0 1,816,638 - +1.5 1,816,638 - +0.3 1,816,638 - -1.2 1,816,638 - -0.4 1,816,671 + -0.9 1,816,671 + -0.9 1,816,671 + -2.7 1,816,671 + -0.5 1,816,672 - -1.3 1,816,672 - -0.5 1,816,674 - -2.3 1,816,674 - -1.0 1,816,674 - +0.5 1,816,766 + +0.1 1,816,766 + -1.9 1,816,890 + Psyr_1614 0.15 +0.7 1,816,890 + Psyr_1614 0.15 -0.2 1,816,890 + Psyr_1614 0.15 +0.3 1,816,891 - Psyr_1614 0.15 -0.3 1,817,202 + Psyr_1614 0.49 +0.5 1,817,202 + Psyr_1614 0.49 +0.1 1,817,203 - Psyr_1614 0.49 -0.7 1,817,315 - Psyr_1614 0.61 +0.8 1,817,315 - Psyr_1614 0.61 +0.1 1,817,358 + Psyr_1614 0.65 -1.7 1,817,525 + Psyr_1614 0.83 -1.0
Or see this region's nucleotide sequence