Experiment: KB with Strychnine Sulfate 10 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Psyr_0995 and Psyr_0996 are separated by 26 nucleotides Psyr_0996 and Psyr_0997 are separated by 41 nucleotides Psyr_0997 and Psyr_0998 are separated by 10 nucleotides Psyr_0998 and Psyr_0999 overlap by 1 nucleotides
Psyr_0995: Psyr_0995 - Flavin reductase-like protein, at 1,129,873 to 1,130,409
_0995
Psyr_0996: Psyr_0996 - Alpha/beta hydrolase fold protein, at 1,130,436 to 1,131,215
_0996
Psyr_0997: Psyr_0997 - Endoribonuclease L-PSP, at 1,131,257 to 1,131,640
_0997
Psyr_0998: Psyr_0998 - Isochorismatase hydrolase, at 1,131,651 to 1,132,388
_0998
Psyr_0999: Psyr_0999 - luciferase, at 1,132,388 to 1,133,470
_0999
Position (kb)
1131
1132 Strain fitness (log2 ratio)
-2
-1
0
1 at 1130.403 kb on + strand at 1130.403 kb on + strand at 1130.403 kb on + strand at 1130.404 kb on - strand at 1130.498 kb on + strand at 1130.498 kb on + strand at 1130.516 kb on - strand, within Psyr_0996 at 1130.793 kb on + strand, within Psyr_0996 at 1130.793 kb on + strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.794 kb on - strand, within Psyr_0996 at 1130.797 kb on + strand, within Psyr_0996 at 1130.797 kb on + strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1130.798 kb on - strand, within Psyr_0996 at 1131.083 kb on - strand, within Psyr_0996 at 1131.083 kb on - strand, within Psyr_0996 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.363 kb on + strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.364 kb on - strand, within Psyr_0997 at 1131.512 kb on + strand, within Psyr_0997 at 1131.512 kb on + strand, within Psyr_0997 at 1131.513 kb on - strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.555 kb on + strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.556 kb on - strand, within Psyr_0997 at 1131.802 kb on + strand, within Psyr_0998 at 1131.802 kb on + strand, within Psyr_0998 at 1131.802 kb on + strand, within Psyr_0998 at 1132.105 kb on + strand, within Psyr_0998 at 1132.105 kb on + strand, within Psyr_0998 at 1132.106 kb on - strand, within Psyr_0998 at 1132.636 kb on + strand, within Psyr_0999
Per-strain Table
Position Strand Gene LocusTag Fraction KB with Strychnine Sulfate 10 mM remove 1,130,403 + -0.1 1,130,403 + -0.7 1,130,403 + +0.3 1,130,404 - +0.1 1,130,498 + +0.5 1,130,498 + -2.1 1,130,516 - Psyr_0996 0.10 +1.6 1,130,793 + Psyr_0996 0.46 -2.0 1,130,793 + Psyr_0996 0.46 -0.0 1,130,794 - Psyr_0996 0.46 -0.3 1,130,794 - Psyr_0996 0.46 -0.7 1,130,794 - Psyr_0996 0.46 -0.8 1,130,794 - Psyr_0996 0.46 -0.5 1,130,797 + Psyr_0996 0.46 -0.7 1,130,797 + Psyr_0996 0.46 -1.5 1,130,798 - Psyr_0996 0.46 -0.4 1,130,798 - Psyr_0996 0.46 +0.4 1,130,798 - Psyr_0996 0.46 +0.7 1,130,798 - Psyr_0996 0.46 +0.1 1,130,798 - Psyr_0996 0.46 -0.6 1,130,798 - Psyr_0996 0.46 +0.4 1,130,798 - Psyr_0996 0.46 +0.5 1,131,083 - Psyr_0996 0.83 -0.7 1,131,083 - Psyr_0996 0.83 +0.4 1,131,363 + Psyr_0997 0.28 -1.1 1,131,363 + Psyr_0997 0.28 +0.1 1,131,363 + Psyr_0997 0.28 -0.0 1,131,363 + Psyr_0997 0.28 -0.8 1,131,363 + Psyr_0997 0.28 -0.5 1,131,364 - Psyr_0997 0.28 -1.0 1,131,364 - Psyr_0997 0.28 +0.3 1,131,364 - Psyr_0997 0.28 -1.5 1,131,364 - Psyr_0997 0.28 -1.1 1,131,512 + Psyr_0997 0.66 -0.3 1,131,512 + Psyr_0997 0.66 -0.9 1,131,513 - Psyr_0997 0.67 +0.1 1,131,555 + Psyr_0997 0.78 -0.1 1,131,555 + Psyr_0997 0.78 -0.4 1,131,555 + Psyr_0997 0.78 -1.4 1,131,555 + Psyr_0997 0.78 -0.4 1,131,555 + Psyr_0997 0.78 +0.2 1,131,555 + Psyr_0997 0.78 -1.8 1,131,556 - Psyr_0997 0.78 -0.8 1,131,556 - Psyr_0997 0.78 +0.1 1,131,556 - Psyr_0997 0.78 +0.3 1,131,556 - Psyr_0997 0.78 +0.6 1,131,556 - Psyr_0997 0.78 +0.8 1,131,556 - Psyr_0997 0.78 -0.2 1,131,556 - Psyr_0997 0.78 -0.4 1,131,556 - Psyr_0997 0.78 -0.3 1,131,556 - Psyr_0997 0.78 -1.4 1,131,802 + Psyr_0998 0.20 +1.3 1,131,802 + Psyr_0998 0.20 -0.7 1,131,802 + Psyr_0998 0.20 +0.2 1,132,105 + Psyr_0998 0.62 -1.0 1,132,105 + Psyr_0998 0.62 +0.2 1,132,106 - Psyr_0998 0.62 -0.1 1,132,636 + Psyr_0999 0.23 -0.5
Or see this region's nucleotide sequence